Chemical transformation buffer comparison: Difference between revisions
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|15mM | |15mM | ||
| | |55mM | ||
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|7% | |7% | ||
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| | |10mM PIPES pH 6.7 | ||
|pH 5.7->6.2 | |pH 5.7->6.2 | ||
|Inoue90 | |Inoue90 | ||
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Revision as of 11:46, 2 May 2006
Buffer | KCl | KCH3COO | CaCl2 | MnCl2 | RbCl | HACoCl3 | Glycerol | DMSO | Sucrose | Other | pH | Reference |
SF | 100mM | 10mM | 50mM | 10% | pH 6.1 | Hanahan patent | ||||||
RFT | 30mM | 10mM | 50mM | 100mM | 15% | pH 5.8 | Hanahan patent | |||||
FSB | 100mM | 10mM | 45mM | 3mM | 10% w/v | pH 6.2 | Hanahan patent | |||||
FSB | 100mM | 10mM | 10mM | 44mM | ? | 3mM | 10% | 2x 3.5% | 5% | pH 6.4->6.2 | Jessee patent | |
FSB | 10mM | 10mM | 45mM | 3mM | 8% | 8% | pH 6.4->6.2 | Stratagene '525 | ||||
CCMB80 | 10mM | 80mM | 20mM | 10% | 7% | 10mM MgCl2 | pH 6.4 | Invitrogen '464 | ||||
TB | 250mM | 15mM | 55mM | 7% | 10mM PIPES pH 6.7 | pH 5.7->6.2 | Inoue90 |