Chemical transformation buffer comparison

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|pH 5.8
|pH 5.8
|Hanahan patent
|Hanahan patent
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|-
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|TFB-I
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|
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|30mM
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|20mM
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|50mM
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|100mM
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|
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|15%
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|
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|
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|
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|pH 5.8
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|
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|-
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|TFB-II
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|
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|
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|75mM
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|
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|10mM
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|
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|
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15%
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|
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|
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|10mM MOPS
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|pH 6.5
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|
|-
|-
|FSB
|FSB

Revision as of 14:17, 2 May 2006

Buffer KCl KCH3COO CaCl2 MnCl2 RbCl HACoCl3 Glycerol DMSO Sucrose Other pH Reference
SF 100mM 10mM 50mM 10% pH 6.1 Hanahan patent
RFT 30mM 10mM 50mM 100mM 15% pH 5.8 Hanahan patent
TFB-I 30mM 20mM 50mM 100mM 15% pH 5.8
TFB-II 75mM 10mM

15%

10mM MOPS pH 6.5
FSB 100mM 10mM 45mM 3mM 10% w/v pH 6.2 Hanahan patent
FSB 100mM 10mM 10mM 44mM ? 3mM 10% 2x 3.5% 5% pH 6.4->6.2 Jessee patent
FSB 10mM 10mM 45mM 3mM 8% 8% pH 6.4->6.2 Stratagene '525
CCMB80 10mM 80mM 20mM 10% 7% 10mM MgCl2 pH 6.4 Invitrogen '464
TB 250mM 15mM 55mM 7% 10mM PIPES pH 6.7 pH 5.7->6.2 Inoue90
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