Chemical transformation buffer comparison

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Line 10: Line 10:
|'''Glycerol'''
|'''Glycerol'''
|'''DMSO'''
|'''DMSO'''
-
|'''Sucrose'''
 
|'''Other'''
|'''Other'''
|'''pH'''
|'''pH'''
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|10%
|10%
-
|
 
|
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|
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|
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|15%
|15%
-
|
 
|
|
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|
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|15%
|15%
-
|
 
|
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|
|
15%
15%
-
|
 
|
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|10mM MOPS
|10mM MOPS
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|3mM
|3mM
|10% w/v
|10% w/v
-
|
 
|
|
|
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|10%
|10%
|2x 3.5%
|2x 3.5%
-
|5%
+
|5% Sucrose
-
|
+
|pH 6.4->6.2
|pH 6.4->6.2
|Jessee patent
|Jessee patent
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|8%
|8%
|8%
|8%
-
|
 
|
|
|pH 6.4->6.2
|pH 6.4->6.2
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|10%
|10%
|7%
|7%
-
|
 
|10mM MgCl<sub>2</sub>
|10mM MgCl<sub>2</sub>
|pH 6.4
|pH 6.4
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|
|
|7%
|7%
-
|
 
|10mM PIPES pH 6.7
|10mM PIPES pH 6.7
|pH 5.7->6.2
|pH 5.7->6.2
|Inoue90
|Inoue90
|}
|}

Revision as of 15:09, 2 May 2006

Buffer KCl KCH3COO CaCl2 MnCl2 RbCl HACoCl3 Glycerol DMSO Other pH Reference
SF 100mM 10mM 50mM 10% pH 6.1 Hanahan patent
RFT 30mM 10mM 50mM 100mM 15% pH 5.8 Hanahan patent
TFB-I 30mM 20mM 50mM 100mM 15% pH 5.8
TFB-II 75mM 10mM

15%

10mM MOPS pH 6.5
FSB 100mM 10mM 45mM 3mM 10% w/v pH 6.2 Hanahan patent
FSB 100mM 10mM 10mM 44mM ? 3mM 10% 2x 3.5% 5% Sucrose pH 6.4->6.2 Jessee patent
FSB 10mM 10mM 45mM 3mM 8% 8% pH 6.4->6.2 Stratagene '525
CCMB80 10mM 80mM 20mM 10% 7% 10mM MgCl2 pH 6.4 Invitrogen '464
TB 250mM 15mM 55mM 7% 10mM PIPES pH 6.7 pH 5.7->6.2 Inoue90
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