Chemical transformation buffer comparison: Difference between revisions

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Buffer   KCl       KCH3COO    CaCl2      MnCl2    RbCl     Glycerol   HACoCl3  DMSO     Sucrose        pH       Ref
{| border="1"
|-
SF         100uM  10uM          50uM                                10%                                                               6.1       Hanahan patent
|'''Buffer'''
RFT                     30uM          10uM      50uM      100uM  15%                                                             5.8       Hanahan patent
|'''KCl'''
FSB       100mM 10mM                       45mM                 10% w/v     3mM         yes                            6.2       Hanahan patent
|'''KOAc'''
|'''CaCl<sub>2</sub>'''
|'''MnCl<sub>2</sub>'''
|'''RbCl'''
|'''HACoCl<sub>3</sub>'''
|'''Glycerol'''
|'''DMSO'''
|'''Other'''
|'''pH'''
|'''Reference'''
|-
|SF
|100mM
| 10mM
| 50mM
|
|
|
|10%
|
|
|pH 6.1
|Hanahan patent
|-
|RFT
|
| 30mM
| 10mM
|50mM
|100mM
|
|15%
|
|
|pH 5.8
|Hanahan patent
|-
|TFB-I
|
|30mM
|20mM
|50mM
|100mM
|
|15%
|
|
|pH 5.8
|
|-
|TFB-II
|
|
|75mM
|
|10mM
|
|
15%
|
|10mM MOPS
|pH 6.5
|
|-
|FSB
|100mM
|10mM
|
|45mM
|
|3mM
|10% w/v
|
|
|pH 6.2
|Hanahan patent
|-
|FSB
|100mM
|10mM
|10mM
|44mM
|?
|3mM
|10%
|2x 3.5%
|5% Sucrose
|pH 6.4->6.2
|Jessee patent


FSB       100mM  10mM       10mM     44mM    ?            10%          3mM       2x 3.5%   5%               6.4 -> 6.2 Jesse patent
|-
FSB        100mM  10mM        10mM      45mM                  8%            3mM        2x 3.5%  5%                6.4        Invitrogen '525
|FSB
    (add 50 uM b-ME and 110mM NaCl, incubate on ice for 10 min, add DNA, 1/2 hr on ice, heat shock)
|
CCMB80             10mM         80mM     20mM                 10%                                                             6.4         Invitrogen '464 (10 mM MgCl2)
|10mM
|10mM
|45mM
|
|3mM
|8%
|8%
|
|pH 6.4->6.2
|Stratagene '525
|-
|CCMB80
|
|10mM
|80mM
|20mM
|
|
|10%
|7%
|10mM MgCl<sub>2</sub>
|pH 6.4
|Invitrogen '464
|-
|TB
|250mM
|
|15mM
|55mM
|
|
|
|7%
|10mM PIPES pH 6.7
|pH 5.7->6.2
|Inoue90
|}

Latest revision as of 18:00, 2 May 2006

Buffer KCl KOAc CaCl2 MnCl2 RbCl HACoCl3 Glycerol DMSO Other pH Reference
SF 100mM 10mM 50mM 10% pH 6.1 Hanahan patent
RFT 30mM 10mM 50mM 100mM 15% pH 5.8 Hanahan patent
TFB-I 30mM 20mM 50mM 100mM 15% pH 5.8
TFB-II 75mM 10mM

15%

10mM MOPS pH 6.5
FSB 100mM 10mM 45mM 3mM 10% w/v pH 6.2 Hanahan patent
FSB 100mM 10mM 10mM 44mM ? 3mM 10% 2x 3.5% 5% Sucrose pH 6.4->6.2 Jessee patent
FSB 10mM 10mM 45mM 3mM 8% 8% pH 6.4->6.2 Stratagene '525
CCMB80 10mM 80mM 20mM 10% 7% 10mM MgCl2 pH 6.4 Invitrogen '464
TB 250mM 15mM 55mM 7% 10mM PIPES pH 6.7 pH 5.7->6.2 Inoue90
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