Chemical transformation buffer comparison

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Buffer   KCl       KCH3COO    CaCl2      MnCl2    RbCl     Glycerol   HACoCl3  DMSO     Sucrose        pH       Ref
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{| border="1"
-
+
|-
-
SF         100uM  10uM          50uM                                10%                                                               6.1       Hanahan patent
+
|'''Buffer'''
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RFT                     30uM          10uM      50uM      100uM  15%                                                             5.8       Hanahan patent
+
|'''KCl'''
-
FSB       100mM 10mM                       45mM                 10% w/v     3mM        yes                            6.2       Hanahan patent
+
|'''KOAc'''
 +
|'''CaCl<sub>2</sub>'''
 +
|'''MnCl<sub>2</sub>'''
 +
|'''RbCl'''
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|'''HACoCl<sub>3</sub>'''
 +
|'''Glycerol'''
 +
|'''DMSO'''
 +
|'''Other'''
 +
|'''pH'''
 +
|'''Reference'''
 +
|-
 +
|SF
 +
|100mM
 +
| 10mM
 +
| 50mM
 +
|
 +
|
 +
|
 +
|10%
 +
|
 +
|
 +
|pH 6.1
 +
|Hanahan patent
 +
|-
 +
|RFT
 +
|
 +
| 30mM
 +
| 10mM
 +
|50mM
 +
|100mM
 +
|
 +
|15%
 +
|
 +
|
 +
|pH 5.8
 +
|Hanahan patent
 +
|-
 +
|TFB-I
 +
|
 +
|30mM
 +
|20mM
 +
|50mM
 +
|100mM
 +
|
 +
|15%
 +
|
 +
|
 +
|pH 5.8
 +
|
 +
|-
 +
|TFB-II
 +
|
 +
|
 +
|75mM
 +
|
 +
|10mM
 +
|
 +
|
 +
15%
 +
|
 +
|10mM MOPS
 +
|pH 6.5
 +
|
 +
|-
 +
|FSB
 +
|100mM
 +
|10mM
 +
|
 +
|45mM
 +
|
 +
|3mM
 +
|10% w/v
 +
|
 +
|
 +
|pH 6.2
 +
|Hanahan patent
 +
|-
 +
|FSB
 +
|100mM
 +
|10mM
 +
|10mM
 +
|44mM
 +
|?
 +
|3mM
 +
|10%
 +
|2x 3.5%
 +
|5% Sucrose
 +
|pH 6.4->6.2
 +
|Jessee patent
-
FSB       100mM  10mM       10mM     44mM    ?            10%          3mM       2x 3.5%   5%               6.4 -> 6.2 Jesse patent
+
|-
-
FSB        100mM  10mM        10mM      45mM                  8%            3mM        2x 3.5%  5%                6.4        Invitrogen '525
+
|FSB
-
    (add 50 uM b-ME and 110mM NaCl, incubate on ice for 10 min, add DNA, 1/2 hr on ice, heat shock)
+
|
-
CCMB80             10mM         80mM     20mM                 10%                                                             6.4         Invitrogen '464 (10 mM MgCl2)
+
|10mM
-
TB         250mM                     15mM     15mM                                                  7%                               6.7 -> 6.2   Inoue
+
|10mM
 +
|45mM
 +
|
 +
|3mM
 +
|8%
 +
|8%
 +
|
 +
|pH 6.4->6.2
 +
|Stratagene '525
 +
|-
 +
|CCMB80
 +
|
 +
|10mM
 +
|80mM
 +
|20mM
 +
|
 +
|
 +
|10%
 +
|7%
 +
|10mM MgCl<sub>2</sub>
 +
|pH 6.4
 +
|Invitrogen '464
 +
|-
 +
|TB
 +
|250mM
 +
|
 +
|15mM
 +
|55mM
 +
|
 +
|
 +
|
 +
|7%
 +
|10mM PIPES pH 6.7
 +
|pH 5.7->6.2
 +
|Inoue90
 +
|}

Current revision

Buffer KCl KOAc CaCl2 MnCl2 RbCl HACoCl3 Glycerol DMSO Other pH Reference
SF 100mM 10mM 50mM 10% pH 6.1 Hanahan patent
RFT 30mM 10mM 50mM 100mM 15% pH 5.8 Hanahan patent
TFB-I 30mM 20mM 50mM 100mM 15% pH 5.8
TFB-II 75mM 10mM

15%

10mM MOPS pH 6.5
FSB 100mM 10mM 45mM 3mM 10% w/v pH 6.2 Hanahan patent
FSB 100mM 10mM 10mM 44mM ? 3mM 10% 2x 3.5% 5% Sucrose pH 6.4->6.2 Jessee patent
FSB 10mM 10mM 45mM 3mM 8% 8% pH 6.4->6.2 Stratagene '525
CCMB80 10mM 80mM 20mM 10% 7% 10mM MgCl2 pH 6.4 Invitrogen '464
TB 250mM 15mM 55mM 7% 10mM PIPES pH 6.7 pH 5.7->6.2 Inoue90
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