Chemical transformation buffer comparison

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Current revision (21:00, 2 May 2006) (view source)
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Line 3: Line 3:
|'''Buffer'''
|'''Buffer'''
|'''KCl'''
|'''KCl'''
-
|'''KCH<sub>3</sub>COO'''
+
|'''KOAc'''
|'''CaCl<sub>2</sub>'''
|'''CaCl<sub>2</sub>'''
|'''MnCl<sub>2</sub>'''
|'''MnCl<sub>2</sub>'''
Line 10: Line 10:
|'''Glycerol'''
|'''Glycerol'''
|'''DMSO'''
|'''DMSO'''
-
|'''Sucrose'''
 
|'''Other'''
|'''Other'''
|'''pH'''
|'''pH'''
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|
|
|10%
|10%
-
|
 
|
|
|
|
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|
|
|15%
|15%
-
|
 
|
|
|
|
|pH 5.8
|pH 5.8
|Hanahan patent
|Hanahan patent
 +
|-
 +
|TFB-I
 +
|
 +
|30mM
 +
|20mM
 +
|50mM
 +
|100mM
 +
|
 +
|15%
 +
|
 +
|
 +
|pH 5.8
 +
|
 +
|-
 +
|TFB-II
 +
|
 +
|
 +
|75mM
 +
|
 +
|10mM
 +
|
 +
|
 +
15%
 +
|
 +
|10mM MOPS
 +
|pH 6.5
 +
|
|-
|-
|FSB
|FSB
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|3mM
|3mM
|10% w/v
|10% w/v
-
|
 
|
|
|
|
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|10%
|10%
|2x 3.5%
|2x 3.5%
-
|5%
+
|5% Sucrose
-
|
+
|pH 6.4->6.2
|pH 6.4->6.2
|Jessee patent
|Jessee patent
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|8%
|8%
|8%
|8%
-
|
 
|
|
|pH 6.4->6.2
|pH 6.4->6.2
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|10%
|10%
|7%
|7%
-
|
+
|10mM MgCl<sub>2</sub>
-
|
+
|pH 6.4
|pH 6.4
|Invitrogen '464
|Invitrogen '464
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|
|
|15mM
|15mM
-
|15mM
+
|55mM
|
|
|
|
|
|
|7%
|7%
-
|
+
|10mM PIPES pH 6.7
-
|
+
|pH 5.7->6.2
|pH 5.7->6.2
|Inoue90
|Inoue90
|}
|}

Current revision

Buffer KCl KOAc CaCl2 MnCl2 RbCl HACoCl3 Glycerol DMSO Other pH Reference
SF 100mM 10mM 50mM 10% pH 6.1 Hanahan patent
RFT 30mM 10mM 50mM 100mM 15% pH 5.8 Hanahan patent
TFB-I 30mM 20mM 50mM 100mM 15% pH 5.8
TFB-II 75mM 10mM

15%

10mM MOPS pH 6.5
FSB 100mM 10mM 45mM 3mM 10% w/v pH 6.2 Hanahan patent
FSB 100mM 10mM 10mM 44mM ? 3mM 10% 2x 3.5% 5% Sucrose pH 6.4->6.2 Jessee patent
FSB 10mM 10mM 45mM 3mM 8% 8% pH 6.4->6.2 Stratagene '525
CCMB80 10mM 80mM 20mM 10% 7% 10mM MgCl2 pH 6.4 Invitrogen '464
TB 250mM 15mM 55mM 7% 10mM PIPES pH 6.7 pH 5.7->6.2 Inoue90
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