Chemical transformation buffer comparison: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
mNo edit summary
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
|'''Buffer'''
|'''Buffer'''
|'''KCl'''
|'''KCl'''
|'''KCH<sub>3</sub>COO'''
|'''KOAc'''
|'''CaCl<sub>2</sub>'''
|'''CaCl<sub>2</sub>'''
|'''MnCl<sub>2</sub>'''
|'''MnCl<sub>2</sub>'''
Line 10: Line 10:
|'''Glycerol'''
|'''Glycerol'''
|'''DMSO'''
|'''DMSO'''
|'''Sucrose'''
|'''Other'''
|'''Other'''
|'''pH'''
|'''pH'''
Line 23: Line 22:
|
|
|10%
|10%
|
|
|
|
|
Line 37: Line 35:
|
|
|15%
|15%
|
|
|
|
|
Line 51: Line 48:
|
|
|15%
|15%
|
|
|
|
|
Line 66: Line 62:
|
|
15%
15%
|
|
|
|10mM MOPS
|10mM MOPS
Line 80: Line 75:
|3mM
|3mM
|10% w/v
|10% w/v
|
|
|
|
|
Line 95: Line 89:
|10%
|10%
|2x 3.5%
|2x 3.5%
|5%
|5% Sucrose
|
|pH 6.4->6.2
|pH 6.4->6.2
|Jessee patent
|Jessee patent
Line 110: Line 103:
|8%
|8%
|8%
|8%
|
|
|
|pH 6.4->6.2
|pH 6.4->6.2
Line 124: Line 116:
|10%
|10%
|7%
|7%
|
|10mM MgCl<sub>2</sub>
|10mM MgCl<sub>2</sub>
|pH 6.4
|pH 6.4
Line 138: Line 129:
|
|
|7%
|7%
|
|10mM PIPES pH 6.7
|10mM PIPES pH 6.7
|pH 5.7->6.2
|pH 5.7->6.2
|Inoue90
|Inoue90
|}
|}

Latest revision as of 18:00, 2 May 2006

Buffer KCl KOAc CaCl2 MnCl2 RbCl HACoCl3 Glycerol DMSO Other pH Reference
SF 100mM 10mM 50mM 10% pH 6.1 Hanahan patent
RFT 30mM 10mM 50mM 100mM 15% pH 5.8 Hanahan patent
TFB-I 30mM 20mM 50mM 100mM 15% pH 5.8
TFB-II 75mM 10mM

15%

10mM MOPS pH 6.5
FSB 100mM 10mM 45mM 3mM 10% w/v pH 6.2 Hanahan patent
FSB 100mM 10mM 10mM 44mM ? 3mM 10% 2x 3.5% 5% Sucrose pH 6.4->6.2 Jessee patent
FSB 10mM 10mM 45mM 3mM 8% 8% pH 6.4->6.2 Stratagene '525
CCMB80 10mM 80mM 20mM 10% 7% 10mM MgCl2 pH 6.4 Invitrogen '464
TB 250mM 15mM 55mM 7% 10mM PIPES pH 6.7 pH 5.7->6.2 Inoue90
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Chemical_transformation_buffer_comparison&oldid=35674"