Chemical transformation buffer comparison
From OpenWetWare
| Buffer | KCl | KCH3COO | CaCl2 | MnCl2 | RbCl | HACoCl3 | Glycerol | DMSO | Sucrose | Other | pH | Reference |
| SF | 100mM | 10mM | 50mM | 10% | pH 6.1 | Hanahan patent | ||||||
| RFT | 30mM | 10mM | 50mM | 100mM | 15% | pH 5.8 | Hanahan patent | |||||
| FSB | 100mM | 10mM | 45mM | 3mM | 10% w/v | pH 6.2 | Hanahan patent | |||||
| FSB | 100mM | 10mM | 10mM | 44mM | ? | 3mM | 10% | 2x 3.5% | 5% | pH 6.4->6.2 | Jessee patent | |
| FSB | 10mM | 10mM | 45mM | 3mM | 8% | 8% | pH 6.4->6.2 | Stratagene '525 | ||||
| CCMB80 | 10mM | 80mM | 20mM | 10% | 7% | 10mM MgCl2 | pH 6.4 | Invitrogen '464 | ||||
| TB | 250mM | 15mM | 15mM | 7% | pH 5.7->6.2 | Inoue90 |
Hanahan suggests that DH5a and derivatives are best prepared chemically with the TSB or FSB buffer protocols, while the MC1061 derivative strains such as DH10B and Top10 are best prepared using CCMB80 protocols. Also, the MC1061 strains should not be grown in Mg++ containing culture media in preparation for transformation.
