Chemical transformation buffer comparison
From OpenWetWare
Buffer | KCl | KCH3COO | CaCl2 | MnCl2 | RbCl | HACoCl3 | Glycerol | DMSO | Sucrose | Other | pH | Reference |
SF | 100mM | 10mM | 50mM | 10% | pH 6.1 | Hanahan patent | ||||||
RFT | 30mM | 10mM | 50mM | 100mM | 15% | pH 5.8 | Hanahan patent | |||||
FSB | 100mM | 10mM | 45mM | 3mM | 10% w/v | pH 6.2 | Hanahan patent | |||||
FSB | 100mM | 10mM | 10mM | 44mM | ? | 3mM | 10% | 2x 3.5% | 5% | pH 6.4->6.2 | Jessee patent | |
FSB | 10mM | 10mM | 45mM | 3mM | 8% | 8% | pH 6.4->6.2 | Stratagene '525 | ||||
CCMB80 | 10mM | 80mM | 20mM | 10% | 7% | 10mM MgCl2 | pH 6.4 | Invitrogen '464 | ||||
TB | 250mM | 15mM | 55mM | 7% | 10mM PIPES pH 6.7 | pH 5.7->6.2 | Inoue90 |