Chloramphenicol: Difference between revisions
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==Working Concentrations and Stock Solutions== | ==Working Concentrations and Stock Solutions== | ||
High copy plasmids allow 35 μg/ml concentration. | High copy plasmids allow 35 μg/ml concentration. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C. | ||
==Resistance Gene== | ==Resistance Gene== | ||
The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it. | The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it. | ||
The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''. | The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''. | ||
Revision as of 12:24, 27 April 2007
Mode of Action
Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosome and inhibits ribosomal peptide bond formation. It is sometimes uses as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.
Working Concentrations and Stock Solutions
High copy plasmids allow 35 μg/ml concentration. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C.
Resistance Gene
The cat gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it. The gene is sometimes specifed as CmR or CamR.
