Chris Rhodes Week 14

Files

Calculations XLS

GenMapp TXT

Increased MAPPFinder Results XLS

Decreased MAPPFinder Results XLS

Increased MAPPFinder Results TXT

Decreased MAPPFinder Results TXT

Materials we need

• Table of significant genes at p< 0.05,0.01,0.001
• Top ten significant genes
• Top ten increased/decreased GO terms
• Make a list of the functions of the top ten genes.
• Take all of this data and hypothesize why under the conditions of the experiment they would be up or down regulated.
• How do your top ten significant genes relate to the GO terms? How do they relate to the experimental conditions?
• How do your results and hypothesis align with the paper you read?

Decreased GO Terms

1. fructuronate reductase activity: Catalysis of the reaction: D-mannonate + NAD(+) = D-fructuronate + H(+) + NADH AmiGO Link
2. precorrin-3B C17-methyltransferase activity: Catalysis of the reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin AmiGO Link
3. precorrin-2 C20-methyltransferase activity:Catalysis of the reaction: S-adenosyl-L-methionine + precorrin-2 = S-adenosyl-L-homocysteine + H(+) + precorrin-3A AmiGO Link
4. coniferyl-aldehyde dehydrogenase activity: Catalysis of the reaction: coniferyl aldehyde + H2O + NAD(P)+ = ferulate + NAD(P)H + H+ AmiGO Link
5. inositol-1(or 4)-monophosphatase activity: Catalysis of the reaction: myo-inositol phosphate + H2O = myo-inositol + phosphate AmiGO Link
6. trans-2-enoyl-CoA reductase (NADPH) activity: Catalysis of the reaction: acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH + H+ AmiGO Link
7. aldehyde dehydrogenase [NAD(P)+] activity: Catalysis of the reaction: an aldehyde + NAD(P)+ + H2O = an acid + NAD(P)H + H+ AmiGO Link
8. oxidoreductase activity:The catalysis of redox reactions specifically relating to energy functions such as NAD+/NADH AmiGO Link
9. inositol or phosphatidylinositol phosphatase activity: Catalysis of the reaction: inositol phosphate(n) + H2O = inositol phosphate(n-1) + phosphate. This reaction is the removal of a phosphate group from an inositol phosphate AmiGO Link
10. cellular aldehyde metabolic process: The chemical reactions and pathways involving aldehydes, any organic compound with the formula R-CH=O, as carried out by individual cells AmiGO Link

Increased GO Terms

1. RNA metabolic process:
2. regulation of transcription:
3. regulation of nitrogen compound metabolic process:
4. regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic process:
5. regulation of macromolecule biosynthetic process:
6. regulation of gene expression:
7. regulation of cellular macromolecule biosynthetic process:
8. regulation of cellular biosynthetic process:
9. regulation of biosynthetic process:
10. regulation of macromolecule metabolic process:

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