Chris Rhodes Week 2: Difference between revisions

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==Methods==
==Methods==
 
#To determine homo- or heterozygous alleles of the parental generation or the original four strands I began by self-crossing each flower. Homozygous crosses would yield only the original color where heterozygous crosses would yield 2 or more colors.
#To determine a scale of dominance among the parental generation and help uncover the different alleles responsible for each color I inter-crossed all the parental flowers.(See Results: Parental Crosses)
#To isolate True-breeding flowers for more accurate future dominance tests I self-crossed select progeny of the F1 generation of the Parental crosses. I then self-crossed select progeny from the F2 generation until a pure F3 generation was created for each color(Red, Blue, Yellow). These True-breeding strains were stored in the Greenhouse.(See Note: Green and White had already been established as true-breeding
#
==Results==
==Results==
'''Parental Crosses'''
'''Parental Crosses'''
*Cross between Red and Red flowers yields: 20 Red and 7 White
*Cross between Green-1 and Green-1 flowers yields: 23 Green
*Cross between Green-2 and Green-2 flowers yields: 12 Green, 6 Yellow, and 5 Blue
*Cross between White and White flowers yields: 17 White
*Cross between Green-1 and White flower yields: 21 Green  
*Cross between Green-1 and White flower yields: 21 Green  
*Cross between Green-2 and White flower yields: 15 Blue and 10 Yellow
*Cross between Green-2 and White flower yields: 15 Blue and 10 Yellow
Line 9: Line 16:
*Cross between Green-1 and Red flowers yields: 11 Black and 8 Green
*Cross between Green-1 and Red flowers yields: 11 Black and 8 Green
*Cross between Green-2 and Red flowers yields: 10 Blue, 9 Purple, 6 Yellow, 2 Orange  
*Cross between Green-2 and Red flowers yields: 10 Blue, 9 Purple, 6 Yellow, 2 Orange  
*Cross between Red and Red flowers yields: 20 Red and 7 White
'''F1 and F2 Crosses to Obtain True-breeding Colors'''
'''F1 and F2 Crosses to Obtain True-breeding colors'''
*Self-cross of Blue-White flower yields: 18 Blue and 10 White
*Self-cross of Blue-White flower yields: 18 Blue and 10 White
*Self-cross of Blue flower progeny of BW X BW yields: 26 Blue
*Self-cross of Blue flower progeny of BW X BW yields: 26 Blue
Line 20: Line 26:
** This F3 progeny was added to the Greenhouse as True-breeding Red
** This F3 progeny was added to the Greenhouse as True-breeding Red
'''True-breeding Crosses to Observe Dominance'''
'''True-breeding Crosses to Observe Dominance'''
*Cross of Green-1 and True-Yellow yields: 26 Green
*Cross of Green-1 and True-Yellow flowers yields: 26 Green
*Cross of Green-1 and True-Blue yields: 23 Green
*Cross of Green-1 and True-Blue flowers yields: 23 Green
*Cross of Green-1 and True-Red yields: 19 Black
*Cross of Green-1 and True-Red flowers yields: 19 Black
*Cross of True-Red and True-Yellow yields: 23 Orange
*Cross of True-Red and True-Yellow flowers yields: 23 Orange
*Cross of True-Red and True-Blue yields: 28 Purple
*Cross of True-Red and True-Blue flowers yields: 28 Purple
*Cross of any True-Color and White yields: 100% True-Color
*Cross of any True-Color and White flowers yields: 100% True-Color
'''Determination of Alleles for Black, Orange, and Purple Flowers'''
*Cross of Black and White flowers yields: 13 Green and 13 Red
*Cross of Orange and White flowers yields: 12 Yellow and 10 Red
*Cross of Purple and White flowers yields: 12 Red and 13 Blue
==Conclusion==
==Conclusion==


==Links==
==Links==
{{Chris H. Rhodes}}
{{Chris H. Rhodes}}

Revision as of 15:39, 7 September 2011

Methods

  1. To determine homo- or heterozygous alleles of the parental generation or the original four strands I began by self-crossing each flower. Homozygous crosses would yield only the original color where heterozygous crosses would yield 2 or more colors.
  2. To determine a scale of dominance among the parental generation and help uncover the different alleles responsible for each color I inter-crossed all the parental flowers.(See Results: Parental Crosses)
  3. To isolate True-breeding flowers for more accurate future dominance tests I self-crossed select progeny of the F1 generation of the Parental crosses. I then self-crossed select progeny from the F2 generation until a pure F3 generation was created for each color(Red, Blue, Yellow). These True-breeding strains were stored in the Greenhouse.(See Note: Green and White had already been established as true-breeding

Results

Parental Crosses

  • Cross between Red and Red flowers yields: 20 Red and 7 White
  • Cross between Green-1 and Green-1 flowers yields: 23 Green
  • Cross between Green-2 and Green-2 flowers yields: 12 Green, 6 Yellow, and 5 Blue
  • Cross between White and White flowers yields: 17 White
  • Cross between Green-1 and White flower yields: 21 Green
  • Cross between Green-2 and White flower yields: 15 Blue and 10 Yellow
    • Both phenotypes were added to Greenhouse as Blue-White and Yellow-White respectively
  • Cross between Red and White flower yields: 12 Red and 12 White
  • Cross between Green-1 and Red flowers yields: 11 Black and 8 Green
  • Cross between Green-2 and Red flowers yields: 10 Blue, 9 Purple, 6 Yellow, 2 Orange

F1 and F2 Crosses to Obtain True-breeding Colors

  • Self-cross of Blue-White flower yields: 18 Blue and 10 White
  • Self-cross of Blue flower progeny of BW X BW yields: 26 Blue
    • This F3 progeny was added to greenhouse as True-breeding Blue
  • Self-cross of Yellow-White flower yields: 16 Yellow and 6 White
  • Self-cross of Yellow flower progeny of YW X YW yields: 25 Yellow
    • This F3 progeny was added to the Greenhouse as True-breeding Yellow
  • Self-Cross of Red progeny of F1 Cross R X R yields: 27 Red
    • This F3 progeny was added to the Greenhouse as True-breeding Red

True-breeding Crosses to Observe Dominance

  • Cross of Green-1 and True-Yellow flowers yields: 26 Green
  • Cross of Green-1 and True-Blue flowers yields: 23 Green
  • Cross of Green-1 and True-Red flowers yields: 19 Black
  • Cross of True-Red and True-Yellow flowers yields: 23 Orange
  • Cross of True-Red and True-Blue flowers yields: 28 Purple
  • Cross of any True-Color and White flowers yields: 100% True-Color

Determination of Alleles for Black, Orange, and Purple Flowers

  • Cross of Black and White flowers yields: 13 Green and 13 Red
  • Cross of Orange and White flowers yields: 12 Yellow and 10 Red
  • Cross of Purple and White flowers yields: 12 Red and 13 Blue

Conclusion

Links

  1. Chris Rhodes User Page
  2. Week 2 Journal
  3. Week 3 Journal
  4. Week 4 Journal
  5. Week 5 Journal
  6. Week 6 Journal
  7. Week 7 Journal
  8. Week 8 Journal
  9. Week 9 Journal
  10. Week 10 Journal
  11. Week 11 Journal
  12. Week 12 Journal
  13. Week 13 Journal
  14. Week 14 Journal
  15. Home Page
  16. Week 5 Assignment Page
  17. Week 6 Assignment Page
  18. Week 7 Assignment Page
  19. Week 8 Assignment Page
  20. Week 9 Assignment Page
  21. Week 10 Assignment Page
  22. Week 11 Assignment Page
  23. Week 12 Assignment Page
  24. Week 13 Assignment Page
  25. Week 14 Assignment Page
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