Chris Rhodes Week 2

Methods

1. To determine homo- or heterozygous alleles of the parental generation or the original four strands I began by self-crossing each flower. Homozygous crosses would yield only the original color where heterozygous crosses would yield 2 or more colors.
2. To determine a scale of dominance among the parental generation and help uncover the different alleles responsible for each color I inter-crossed all the parental flowers.(See Results: Parental Crosses)
3. To isolate True-breeding flowers for more accurate future dominance tests I self-crossed select progeny of the F1 generation of the Parental crosses. I then self-crossed select progeny from the F2 generation until a pure F3 generation was created for each color(Red, Blue, Yellow). These True-breeding strains were stored in the Greenhouse.(See Results:F1 and F2 Crosses...) Note: Green and White had already been established as true-breeding
4. To measure a scale of domincance among all the alleles I inter-crossed all the True-breeding color. (See Results: True-breeding Crosses...)
5. To determine the individual alleles that made up each non-true-breeding color (Purple, Orange, Black) I crossed each non-true-breeding color with White. (See Results: Determination of Alleles...)

Results

Parental Crosses

• Cross between Red and Red flowers yields: 20 Red and 7 White
• Cross between Green-1 and Green-1 flowers yields: 23 Green
• Cross between Green-2 and Green-2 flowers yields: 12 Green, 6 Yellow, and 5 Blue
• Cross between White and White flowers yields: 17 White
• Cross between Green-1 and White flower yields: 21 Green
• Cross between Green-2 and White flower yields: 15 Blue and 10 Yellow
  • Both phenotypes were added to Greenhouse as Blue-White and Yellow-White respectively
• Cross between Red and White flower yields: 12 Red and 12 White
• Cross between Green-1 and Red flowers yields: 11 Black and 8 Green
• Cross between Green-2 and Red flowers yields: 10 Blue, 9 Purple, 6 Yellow, 2 Orange

F1 and F2 Crosses to Obtain True-breeding Colors

• Self-cross of Blue-White flower yields: 18 Blue and 10 White
• Self-cross of Blue flower progeny of BW X BW yields: 26 Blue
  • This F3 progeny was added to greenhouse as True-breeding Blue
• Self-cross of Yellow-White flower yields: 16 Yellow and 6 White
• Self-cross of Yellow flower progeny of YW X YW yields: 25 Yellow
  • This F3 progeny was added to the Greenhouse as True-breeding Yellow
• Self-Cross of Red progeny of F1 Cross R X R yields: 27 Red
  • This F3 progeny was added to the Greenhouse as True-breeding Red

True-breeding Crosses to Observe Dominance

• Cross of Green-1 and True-Yellow flowers yields: 26 Green
• Cross of Green-1 and True-Blue flowers yields: 23 Green
• Cross of Green-1 and True-Red flowers yields: 19 Black
• Cross of True-Red and True-Yellow flowers yields: 23 Orange
• Cross of True-Red and True-Blue flowers yields: 28 Purple
• Cross of any True-Color and White flowers yields: 100% True-Color

Determination of Alleles for Black, Orange, and Purple Flowers

• Cross of Black and White flowers yields: 13 Green and 13 Red
• Cross of Orange and White flowers yields: 12 Yellow and 10 Red
• Cross of Purple and White flowers yields: 12 Red and 13 Blue

Conclusion

After reviewing the results from today's experiment a few conclusions can be drawn. Firstly, the 8 colors of flowers

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