Chris Rhodes Week 3: Difference between revisions
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'''Activity 2 Part 2''' | '''Activity 2 Part 2''' | ||
*To calculate the S value of a particular alignment I selected all of Subject 8's clones and ran a ClustalW alignment. | *To calculate the S value of a particular alignment I selected all of Subject 8's clones and ran a ClustalW alignment. | ||
[[Image:CHR_Image5_20110914.jpg|Subject 8 Alignment]]This was also done for Subject 9 | [[Image:CHR_Image5_20110914.jpg|Subject 8 Alignment]] | ||
This was also done for Subject 9 | |||
[[Image:CHR_Image6_20110914.jpg|Subject 9 Alignment]] | [[Image:CHR_Image6_20110914.jpg|Subject 9 Alignment]] | ||
and Subject 10 | and Subject 10 |
Revision as of 17:02, 14 September 2011
Methods
Activity 1 Part 2 and 3
- From GenBank I added the Fasta sequence of AF016760, AFO016761, AFO016767, AFO016780, and AFO016787 to the WorkBench nucleotide tools, ran a ClustalW multiple sequence allignment using the default parameters:
- I also looked at the unrooted genetic tree and took a screenshot:
Activity 2 Part 1
- I added the nucleic acid sequence of both .txt files on the WorkBench.
- From the sequences I ran a ClustalW allignment of: S12V1-1, S12V1-2, S12V1-3, S13V1-2, S13V1-3, S13V1-4, S14V1-4, S14V1-5, S14V1-6, S15V1-10, S15V1-11, S15V1-12 and took a screenshot of the alignment and the unrooted genetic tree:
Activity 2 Part 2
- To calculate the S value of a particular alignment I selected all of Subject 8's clones and ran a ClustalW alignment.
This was also done for Subject 9 and Subject 10
- From these alignments S and Theta were calculated and using Clustdist for each alignment the Min and Max differences were determined. (See Results: Activity 2 Part 2: Analysis Table)
- Using Subjects 12 and 13 I created a new alignment to check the Min and Max differences across subjects by using Clustdist
- This was repeated using a Subject 8 and 9 alignment and a Subject 8 and 10 alignment.
- Using the Clustdist of all the subject cross alignments the Min and Max differences for each were calculated. (See Results: Activity 2, Part 2: Cross Subject Alignment Table)
Results
Activity 2, Part 2: Single Subject Alignment Table
Subject Clones S Theta Min-Diff. Max-Diff. 8 5 6 2.88 1.14 5.13 9 5 5 2.44 1.99 3.99 10 7 7 2.86 1.14 3.14
Activity 2, Part 2: Cross Subject Alignment Table
Subject Crosses Min-Diff. Max-Diff. 12 and 13 39.05 43.89 8 and 9 27.93 33.06 8 and 10 27.1 31.07
Questions
Activity 1, Part 2: GenBank
- AF016760, AFO016761, AFO016767, AFO016780, and AFO016787
- These gene sequences all came from subject 1
Activity 2, Part 1
- Clones from each subject tend to cluster together on the unrooted genetic tree
- Some subject clusters are much closer to each other on the tree than others specifically Subjects 13,14,and 15
- From the pattern of clustering seen in the genetic tree it seems that the genetic tree separates individual sequences based on their similarity to each other. The closer one sequence is to another on the tree, the more closely those two sequences are related to each other in terms of sequence similarity or evolutionary time.
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