Chris Rhodes Week 9: Difference between revisions
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*Individual ClustalW alignments were performed between all the amino acid sequences of a particular subject and the amino acid sequences of the proteins used in the Kwong and Stanfield papers in order to identify regions of possible functional significance in the amino acid sequences of the subjects. The Kwong and Stanfield sequences can be found here: [http://www.ncbi.nlm.nih.gov/protein/3319095?report=fasta Kwong], [http://www.ncbi.nlm.nih.gov/protein/6729933?report=fasta Stanfield 1999 Aib], [http://www.ncbi.nlm.nih.gov/protein/6729961?report=fasta Stanfield 1999 His/Ser Loop], [http://www.ncbi.nlm.nih.gov/protein/39654246?report=fasta Stanfield 2003] | *Individual ClustalW alignments were performed between all the amino acid sequences of a particular subject and the amino acid sequences of the proteins used in the Kwong and Stanfield papers in order to identify regions of possible functional significance in the amino acid sequences of the subjects. The Kwong and Stanfield sequences can be found here: [http://www.ncbi.nlm.nih.gov/protein/3319095?report=fasta Kwong], [http://www.ncbi.nlm.nih.gov/protein/6729933?report=fasta Stanfield 1999 Aib], [http://www.ncbi.nlm.nih.gov/protein/6729961?report=fasta Stanfield 1999 His/Ser Loop], [http://www.ncbi.nlm.nih.gov/protein/39654246?report=fasta Stanfield 2003] | ||
*Regions of Functional significance were determined as highly conserved consecutive sequence areas in the alignments between the subject and the Kwong and Stanfield sequences. Examples of these regions for Subject 13 are shown below: | *Regions of Functional significance were determined as highly conserved consecutive sequence areas in the alignments between the subject and the Kwong and Stanfield sequences. Examples of these regions for Subject 13 are shown below: | ||
[[Image:CHR_Image1_20111026.jpg]][[Image:CHR_Image2_20111026]] | [[Image:CHR_Image1_20111026.jpg]][[Image:CHR_Image2_20111026.jpg]] | ||
Revision as of 14:34, 26 October 2011
Methods
- In order to work with the amino acid sequences of the subjects from the Markham paper each subject's amino acid data was uploaded onto Workbench
- Individual ClustalW alignments were performed between all the amino acid sequences of a particular subject and the amino acid sequences of the proteins used in the Kwong and Stanfield papers in order to identify regions of possible functional significance in the amino acid sequences of the subjects. The Kwong and Stanfield sequences can be found here: Kwong, Stanfield 1999 Aib, Stanfield 1999 His/Ser Loop, Stanfield 2003
- Regions of Functional significance were determined as highly conserved consecutive sequence areas in the alignments between the subject and the Kwong and Stanfield sequences. Examples of these regions for Subject 13 are shown below:
