Christiaen:Methods

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(New page: {{template:christiaen}} ''page in construction''<br> <div style="padding: 5px; width: 732px; border: 4px solid #191970;"> this page provides access to the methods referred to as in the [[...)
Current revision (10:32, 18 February 2010) (view source)
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this page provides access to the methods referred to as in the [[beginner's guide to ascidian developmental biology]] as well as other resources.
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this page provides access to the methods referred to as in the ''[[beginner's guide to ascidian developmental biology]]'' as well as other resources.
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==animals and embryos==
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[[sea water tank and adult animals]]<br>
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Most of the methods are described in details in the [http://cshprotocols.cshlp.org/cgi/content/abstract/2009/12/pdb.emo138 CSHL press - Ciona protocols], including :<br>
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[[dissection and fertilization]]<br>
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[[dechorionnation]]<br>
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[[Ciona electroporation]]<br>
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[[microinjection]]<br>
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[[fluorescence activated cell sorting (FACS)]]
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<br>
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==molecular biology==
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[[molecular cloning]]<br>
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[[microarray analysis]]<br>
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<br>
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==immunohistochemistry and ''in situ'' hybridization==
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<br>
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==microscopy and image analysis==
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<br>

Current revision

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page in construction

this page provides access to the methods referred to as in the beginner's guide to ascidian developmental biology as well as other resources.

Contents

animals and embryos

sea water tank and adult animals
Most of the methods are described in details in the CSHL press - Ciona protocols, including :
dissection and fertilization
dechorionnation
Ciona electroporation
microinjection
fluorescence activated cell sorting (FACS)

molecular biology

molecular cloning
microarray analysis

immunohistochemistry and in situ hybridization


microscopy and image analysis


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