Christiaen:Methods: Difference between revisions
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==animals and embryos== | ==animals and embryos== | ||
[[sea water tank and adult animals]] | [[sea water tank and adult animals]]<br> | ||
Most of the methods are described in details in the [http://cshprotocols.cshlp.org/cgi/content/abstract/2009/12/pdb.emo138 CSHL press - Ciona protocols], including :<br> | |||
[[dissection and fertilization]]<br> | [[dissection and fertilization]]<br> | ||
[[dechorionnation]]<br> | [[dechorionnation]]<br> | ||
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[[fluorescence activated cell sorting (FACS)]] | [[fluorescence activated cell sorting (FACS)]] | ||
<br> | <br> | ||
==molecular biology== | ==molecular biology== | ||
[[molecular cloning]]<br> | [[molecular cloning]]<br> | ||
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==immunohistochemistry and ''in situ'' hybridization== | ==immunohistochemistry and ''in situ'' hybridization== | ||
<br> | <br> | ||
== | ==microscopy and image analysis== | ||
<br> | <br> | ||
Latest revision as of 07:32, 18 February 2010
page in construction
this page provides access to the methods referred to as in the beginner's guide to ascidian developmental biology as well as other resources.
animals and embryos
sea water tank and adult animals
Most of the methods are described in details in the CSHL press - Ciona protocols, including :
dissection and fertilization
dechorionnation
Ciona electroporation
microinjection
fluorescence activated cell sorting (FACS)
molecular biology
molecular cloning
microarray analysis
immunohistochemistry and in situ hybridization
microscopy and image analysis
