Christiaen:Methods

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==animals and embryos==
==animals and embryos==
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[[sea water tank and adult animals]]
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[[sea water tank and adult animals]]<br>
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Most of the methods are described in details in the [http://cshprotocols.cshlp.org/cgi/content/abstract/2009/12/pdb.emo138 CSHL press - Ciona protocols], including :<br>
[[dissection and fertilization]]<br>
[[dissection and fertilization]]<br>
[[dechorionnation]]<br>
[[dechorionnation]]<br>
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[[fluorescence activated cell sorting (FACS)]]
[[fluorescence activated cell sorting (FACS)]]
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<br>
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==molecular biology==
==molecular biology==
[[molecular cloning]]<br>
[[molecular cloning]]<br>
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==immunohistochemistry and ''in situ'' hybridization==
==immunohistochemistry and ''in situ'' hybridization==
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<br>
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==imaging and image analysis==
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==microscopy and image analysis==
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Current revision

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page in construction

this page provides access to the methods referred to as in the beginner's guide to ascidian developmental biology as well as other resources.

Contents

animals and embryos

sea water tank and adult animals
Most of the methods are described in details in the CSHL press - Ciona protocols, including :
dissection and fertilization
dechorionnation
Ciona electroporation
microinjection
fluorescence activated cell sorting (FACS)

molecular biology

molecular cloning
microarray analysis

immunohistochemistry and in situ hybridization


microscopy and image analysis


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