Christiaen:Methods
From OpenWetWare
page in construction
this page provides access to the methods referred to as in the beginner's guide to ascidian developmental biology as well as other resources.
animals and embryos
sea water tank and adult animals
Most of the methods are described in details in the CSHL press - Ciona protocols, including :
dissection and fertilization
dechorionnation
Ciona electroporation
microinjection
fluorescence activated cell sorting (FACS)
molecular biology
molecular cloning
microarray analysis
immunohistochemistry and in situ hybridization
microscopy and image analysis
