Christiaen:Methods

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page in construction

this page provides access to the methods referred to as in the beginner's guide to ascidian developmental biology as well as other resources.

animals and embryos

sea water tank and adult animals dissection and fertilization
dechorionnation
electroporation
microinjection
fluorescence activated cell sorting (FACS)

molecular biology

molecular cloning microarray analysis

immunohistochemistry and in situ hybridization

imaging and image analysis

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