Christiaen:Methods

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page in construction

this page provides access to the methods referred to as in the beginner's guide to ascidian developmental biology as well as other resources.

Contents

animals and embryos

sea water tank and adult animals
dissection and fertilization
dechorionnation
Ciona electroporation
microinjection
fluorescence activated cell sorting (FACS)

molecular biology

molecular cloning
microarray analysis

immunohistochemistry and in situ hybridization


microscopy and image analysis


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