Christian Niederauer/Notebook/RacingBacteria/Protocols/Bacteria/Glycerol Protocol: Difference between revisions
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(New page: ==Reviving Bacteria from Glycerol Stock== * Scrape the frozen surface of the culture with a sterile inoculating needle, and then immediately [Christian Niederauer/Notebook/RacingBacteria/P...) |
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==Reviving Bacteria from Glycerol Stock== | ==Reviving Bacteria from Glycerol Stock== | ||
* Scrape the frozen surface of the culture with a sterile inoculating needle, and then immediately [Christian Niederauer/Notebook/RacingBacteria/Protocols/Bacteria/Spreading_Bacteria streak the bacteria] that adhere to the needle onto the surface of an LB agar plate containing the appropriate antibiotics. Incubate the plate overnight at 37°C | * Scrape the frozen surface of the culture with a sterile inoculating needle, and then immediately [[Christian Niederauer/Notebook/RacingBacteria/Protocols/Bacteria/Spreading_Bacteria | streak the bacteria]] that adhere to the needle onto the surface of an LB agar plate containing the appropriate antibiotics. Incubate the plate overnight at 37°C | ||
* Scrape the frozen surface of the culture with a sterile inoculating needle or tip, and immediately immerse it in 2ml growth media within a 2059 snap-cap tube. Grow the bacteria overnight in a 37°C shaker. | * Scrape the frozen surface of the culture with a sterile inoculating needle or tip, and immediately immerse it in 2ml growth media within a 2059 snap-cap tube. Grow the bacteria overnight in a 37°C shaker. | ||
* Return the frozen culture to storage at -70°C | * Return the frozen culture to storage at -70°C | ||
Source: [http://cshprotocols.cshlp.org/content/2006/1/pdb.prot4452 doi:10.1101/pdb.prot4452] | Source: [http://cshprotocols.cshlp.org/content/2006/1/pdb.prot4452 doi:10.1101/pdb.prot4452] | ||
Revision as of 11:00, 23 August 2014
Reviving Bacteria from Glycerol Stock
- Scrape the frozen surface of the culture with a sterile inoculating needle, and then immediately streak the bacteria that adhere to the needle onto the surface of an LB agar plate containing the appropriate antibiotics. Incubate the plate overnight at 37°C
- Scrape the frozen surface of the culture with a sterile inoculating needle or tip, and immediately immerse it in 2ml growth media within a 2059 snap-cap tube. Grow the bacteria overnight in a 37°C shaker.
- Return the frozen culture to storage at -70°C
Source: doi:10.1101/pdb.prot4452
