Colony PCR: Difference between revisions
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==General Information== | ==General Information== | ||
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*[[Endy:Colony PCR]] | *[[Endy:Colony PCR]] | ||
*[[Knight:Colony PCR]] | *[[Knight:Colony PCR]] | ||
*[[McClean:_Colony_PCR_(Yeast)]] | |||
*[[Silver:_Colony_PCR]] | |||
*[[Blackburn:Yeast_Colony_PCR]] | |||
==Notes== | ==Notes== | ||
#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb. | #I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb. | ||
[[Category:Protocol]] | |||
[[Category:Escherichia coli]] | |||
[[Category:DNA]] | |||
Latest revision as of 03:39, 1 July 2012
| back to protocols | ||
General Information
Colony PCR is can be used after a transformation to screen colonies for the desired plasmid. Primers are used which generate a PCR product of known size. Thus, any colonies which give rise to an amplification product of the expected size are likely to contain the correct DNA sequence.
Specific Protocols
- Endy:Colony PCR
- Knight:Colony PCR
- McClean:_Colony_PCR_(Yeast)
- Silver:_Colony_PCR
- Blackburn:Yeast_Colony_PCR
Notes
- I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.
