Cong T. Trinh:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 6: | Line 6: | ||
[http://openwetware.org/wiki/Cong_T._Trinh:Ligation Ligation].....................................[http://openwetware.org/wiki/Cong_T._Trinh:Taq_PCR Taq PCR] | [http://openwetware.org/wiki/Cong_T._Trinh:Ligation Ligation].....................................[http://openwetware.org/wiki/Cong_T._Trinh:Taq_PCR Taq PCR] | ||
[http://openwetware.org/wiki/Cong_T._Trinh:P1transduction#P1_transduction P1 Transduction]..............................[http://openwetware.org/wiki/Cong_T._Trinh:Pfu_ultra_hotstart Pfu ultra hotstart gene amplification ] | [http://openwetware.org/wiki/Cong_T._Trinh:P1transduction#P1_transduction P1 Transduction]..............................[http://openwetware.org/wiki/Cong_T._Trinh:Pfu_ultra_hotstart Pfu ultra hotstart gene amplification ] | ||
[http://openwetware.org/wiki/Cong_T._Trinh:mini_prep#Protocol Mini Prep].................................... | [http://openwetware.org/wiki/Cong_T._Trinh:mini_prep#Protocol Mini Prep]....................................[http://openwetware.org/wiki/Cong_T._Trinh:electrophoresis#DNA_Gel_Electrophoresis] | ||
===Standard Operating Procedures=== | ===Standard Operating Procedures=== | ||
Revision as of 15:26, 30 November 2011
Techniques
AdhE enzyme assay on aldehydes ............. Analysis of fatty acids from total lipids Cell free preparation....................... Double Digestion Electroporation..............................Heat Shock Ligation.....................................Taq PCR P1 Transduction..............................Pfu ultra hotstart gene amplification Mini Prep....................................[1]
Standard Operating Procedures
HPLC Chemical Competent
Media Preperation
Media for E. coli Media for Acetobactor aceti Media for P. putida Media for Z. mobilis
Buffers and others
Buffers
Updated 24 Aug. 2011 MW
