Construction Files
From OpenWetWare
Gene Synthesis of B09ig1 {?azo1653 AtD?}
Pool O09ig1 through O09ig26, assemble by PCA PCR O09ig1/O09ig26 on PCA reaction (860, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig1 {?azo1653 AtD?} ---- O09ig1 PCA assembly of {?azo1653 AtD?} (B09ig1) CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC O09ig2 PCA assembly of {?azo1653 AtD?} (B09ig1) TAGCACCAGCGTTACCGTCGTCGTTCATACGCTGAGTGCCAGAAGACATCTGG O09ig3 PCA assembly of {?azo1653 AtD?} (B09ig1) CGACGGTAACGCTGGTGCTACCACCCAGGACTTCGACGGTGCTCTGATCGGTG O09ig4 PCA assembly of {?azo1653 AtD?} (B09ig1) CAACACGCCAGTCACCGAAAGCCGGCAGGTCACCACCGATCAGAGCACCGTCG O09ig5 PCA assembly of {?azo1653 AtD?} (B09ig1) TTTCGGTGACTGGCGTGTTGGTGCTGCTATCGGTTACGACATCGCTCAGGTTT O09ig6 PCA assembly of {?azo1653 AtD?} (B09ig1) TGGTTTCACCCTCTGCCTGACGAGAACCAACAGAAACCTGAGCGATGTCGTAA O09ig7 PCA assembly of {?azo1653 AtD?} (B09ig1) TCAGGCAGAGGGTGAAACCAAAACCCGTCGTTTCGCTCTGTACTCTGGTAAAT O09ig8 PCA assembly of {?azo1653 AtD?} (B09ig1) CACGCAGAGAGTTACCGTCAGCCAGTTCGAAAGATTTACCAGAGTACAGAGCG O09ig9 PCA assembly of {?azo1653 AtD?} (B09ig1) TGACGGTAACTCTCTGCGTGTTTTCGGTGGTGCTGCTTACTCTCTGCACCGTC O09ig10 PCA assembly of {?azo1653 AtD?} (B09ig1) CGTCGATCAGAGAAACGTCACGAGCAGATTCCAGACGGTGCAGAGAGTAAGCA O09ig11 PCA assembly of {?azo1653 AtD?} (B09ig1) TGACGTTTCTCTGATCGACGGTGTTGAACACCTGACCCGTGGTTACGACGTTG O09ig12 PCA assembly of {?azo1653 AtD?} (B09ig1) GGTAAGCCAGTTCACCGAAGAGTTGACGGGTAACAACGTCGTAACCACGGGTC O09ig13 PCA assembly of {?azo1653 AtD?} (B09ig1) CTTCGGTGAACTGGCTTACCGTCTGGGTCTGGGTGAAAAATCTCACGTTGAAC O09ig14 PCA assembly of {?azo1653 AtD?} (B09ig1) CACGCTGAGCAACACGCATCAGACCAACGAACGGTTCAACGTGAGATTTTTCA O09ig15 PCA assembly of {?azo1653 AtD?} (B09ig1) GATGCGTGTTGCTCAGCGTGCTGACGGTTTCCAGGAAGAAGGTGGTTCTGCTG O09ig16 PCA assembly of {?azo1653 AtD?} (B09ig1) GCAGAGCGTTACGCTGAGAGTCACCAGCCAGAGCAGCAGAACCACCTTCTTCC O09ig17 PCA assembly of {?azo1653 AtD?} (B09ig1) CTCTCAGCGTAACGCTCTGCTGATCTCTACCCTGGGTGCTCGTGGTCAGCACG O09ig18 PCA assembly of {?azo1653 AtD?} (B09ig1) TCAGTTCGATGTCCAGACCACCCAGGTTAACAGCGTGCTGACCACGAGCACCC O09ig19 PCA assembly of {?azo1653 AtD?} (B09ig1) TGGTCTGGACATCGAACTGAAAGGTTCTGTTACCTGGCGTCACCTGGAAGGTG O09ig20 PCA assembly of {?azo1653 AtD?} (B09ig1) CAGACAGACGCAGACCCAGTTCCGGACGAACGTCACCTTCCAGGTGACGCCAG O09ig21 PCA assembly of {?azo1653 AtD?} (B09ig1) ACTGGGTCTGCGTCTGTCTGGTGGTGCTCGTTTCACCGTTATGGGCACTGAAC O09ig22 PCA assembly of {?azo1653 AtD?} (B09ig1) CGTTCAGGGTCAGCAGCATAGAGTCACGCGGCAGTTCAGTGCCCATAACGGTG O09ig23 PCA assembly of {?azo1653 AtD?} (B09ig1) TATGCTGCTGACCCTGAACGCTAACTACGACCTGATGGCTGGTGTTGTTCTGA O09ig24 PCA assembly of {?azo1653 AtD?} (B09ig1) CAGAGTCAGAGGTCATAGCGTTAACACCAACGTTCAGAACAACACCAGCCATC O09ig25 PCA assembly of {?azo1653 AtD?} (B09ig1) CGCTATGACCTCTGACTCTGGTAACGCTGCTGCTCTGGTTGCTAACCTGCGCT O09ig26 PCA assembly of {?azo1653 AtD?} (B09ig1) CGTTAGGATCCGTTCATTTTCCAGCGCAGGTTAGCAACCAGA
Gene Synthesis of B09ig27 {?Nmul_A1566 AtD?}
Pool O09ig27 through O09ig56, assemble by PCA PCR O09ig27/O09ig56 on PCA reaction (848, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig27 {?Nmul_A1566 AtD?} ---- O09ig27 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CCATAGAATTCATGAGATCTGCTTTCTGGACCCGTACCTTCGGTTC O09ig28 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GTTCATGTCACCAGAACGGTGACCGAAAGAACCGAAGGTACGGGTCCA O09ig29 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCGTTCTGGTGACATGAACGCTGCTCGTATCAACCGTAACATCGGTG O09ig30 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCAGGGTGTCACCACCCATGAAGAAACCACCGATGTTACGGTTGATA O09ig31 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ATGGGTGGTGACACCCTGGTTGCTGACCTGCTGCGTCTGGGTATCGCT O09ig32 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CGTTGAAAGAAGAGTTAGAGTAACCACCAGCGATACCCAGACGCAGCA O09ig33 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CTCTAACTCTTCTTTCAACGTTAACGAACGTTTCTCTACTGGCTCTTC O09ig34 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCGTAAGCGGTAACGTGGTAGTTGTCAGAAGAGCCAGTAGAGAAACG O09ig35 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCACGTTACCGCTTACGGTGGCACTCGTTGGCACGCTCTGGGTCTGC O09ig36 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TCGTGCCAGGTGTAACCACCACCGAAACGCAGACCCAGAGCGTGCCAA O09ig37 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GGTGGTTACACCTGGCACGACTTCGAAACCAACCGTAATATTCTGTTT O09ig38 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CACCTTTAGCCTGGTTGTTGAAACCTGGAAACAGAATATTACGGTTGG O09ig39 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CAACAACCAGGCTAAAGGTGACTACCGTGGTCGTACTGGCCAGGTTTT O09ig40 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GAACGGCAGTTCGTAACCCAGTTCACCGAAAACCTGGCCAGTACGACC O09ig41 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TGGGTTACGAACTGCCGTTCAAAAACGTTTCTCTGGAACCGTTCGCTG O09ig42 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TTGGTAGCCAGGTTAACGTAAGCAACACCAGCGAACGGTTCCAGAGAA O09ig43 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TACGTTAACCTGGCTACCAAAGGTTTCCAGGAACGTGGTGGTATCGCT O09ig44 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CTTCGTTGTCACGAGAAGAAGACAGAGCAGCGATACCACCACGTTCCT O09ig45 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TTCTTCTCGTGACAACGAAGGTGTTACCTACTCTACCGTTGGTATGAA O09ig46 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCAGCGGTAGAGAACATAGAAGCAGCGTTCATACCAACGGTAGAGTA O09ig47 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CTATGTTCTCTACCGCTGGTGGCACTACCACCCGTCTGCGTGGTTCTC O09ig48 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACAGAGTCGAAAGCGTGACGCCAACCCAGAGAACCACGCAGACGGGTG O09ig49 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CGTCACGCTTTCGACTCTGTTCCGACCCGTTCTTCTGTTGCTTTCTCT O09ig50 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TGCCAGCGATACCGAAAGCAGAACCACCAGAGAAAGCAACAGAAGAAC O09ig51 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TGCTTTCGGTATCGCTGGCACTCCGATCGCTAAAGACGCTATGATCAT O09ig52 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCAACAGAAGCGTCCAGACCAGCACCGATGATCATAGCGTCTTTAGC O09ig53 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GTCTGGACGCTTCTGTTGGTAAAAACGCTATCCTGGGTATCGCTTACA O09ig54 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TCAACAACGTTAGAGAAAACCTGGCCAGTGTAAGCGATACCCAGGATA O09ig55 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GTTTTCTCTAACGTTGTTGACAACGGTGTTCGTGCTAACCTGGACTGG O09ig56 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CGTTAGGATCCGAAACGCCAGTCCAGGTTAGCACGAA
Gene Synthesis of B09ig57 {?OprF AtD?}
Pool O09ig57 through O09ig78, assemble by PCA PCR O09ig57/O09ig78 on PCA reaction (710, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig57 {?OprF AtD?} ---- O09ig57 PCA assembly of {?OprF AtD?} (B09ig57) CCATAGAATTCATGAGATCTGACTCTAAAATGACTGGCGCTTACGGTC O09ig58 PCA assembly of {?OprF AtD?} (B09ig57) AGAGGTAGAGTCAGAGATACCTTTTTCGATCTGACCGTAAGCGCCAGTCATT O09ig59 PCA assembly of {?OprF AtD?} (B09ig57) GTATCTCTGACTCTACCTCTGTTGGTCTGATGCTGGGTGGTAACAACAACAA O09ig60 PCA assembly of {?OprF AtD?} (B09ig57) TACCTTTCAGGTCAGAGGTAGCGATGTCAACTTTGTTGTTGTTACCACCCAG O09ig61 PCA assembly of {?OprF AtD?} (B09ig57) TACCTCTGACCTGAAAGGTAACTCTGCTTACCTGGGTGGTTACGTTAAACAC O09ig62 PCA assembly of {?OprF AtD?} (B09ig57) ATACCAGCGATACCACGGAAAGCACCTTTGTCGTGTTTAACGTAACCACCCA O09ig63 PCA assembly of {?OprF AtD?} (B09ig57) TTCCGTGGTATCGCTGGTATCGGTATCCAGTACTCTGACTGGGACTCTCACC O09ig64 PCA assembly of {?OprF AtD?} (B09ig57) GTTTTCTTTGTAAGAGTCAGACATGGTGTTACGGTGAGAGTCCCAGTCAGAG O09ig65 PCA assembly of {?OprF AtD?} (B09ig57) CTGACTCTTACAAAGAAAACTACTCTGACCGTGGTCTGAACATCTACGCAGA O09ig66 PCA assembly of {?OprF AtD?} (B09ig57) GACCTTCGGTCAGTTCTTTGGTGTATTTACCCTCTGCGTAGATGTTCAGACC O09ig67 PCA assembly of {?OprF AtD?} (B09ig57) CAAAGAACTGACCGAAGGTCTGTTCCTGGAACCGAAAGTTGGTGTTTCTTAC O09ig68 PCA assembly of {?OprF AtD?} (B09ig57) GATTCGTTGATAGATTCCTGTTTGATGTAGTCGTAAGAAACACCAACTTTCG O09ig69 PCA assembly of {?OprF AtD?} (B09ig57) CAGGAATCTATCAACGAATCTAAAGACAAAGCTCTGGCTCTGGAAGTTGACG O09ig70 PCA assembly of {?OprF AtD?} (B09ig57) AACGTTACCAGACAGGGTGTCGAAGTCTTTACCGTCAACTTCCAGAGCCAGA O09ig71 PCA assembly of {?OprF AtD?} (B09ig57) ACACCCTGTCTGGTAACGTTGGTCTGGACCTGCGTAAAGAAATCGTTACCGA O09ig72 PCA assembly of {?OprF AtD?} (B09ig57) TAACACCAGCGGTCAGGTTGTGTTTACCTTTTTCGGTAACGATTTCTTTACG O09ig73 PCA assembly of {?OprF AtD?} (B09ig57) CAACCTGACCGCTGGTGTTAACTACACCCGTATCCTGTCTGGTGCTGACGAA O09ig74 PCA assembly of {?OprF AtD?} (B09ig57) GAGTTACCACCGAAGTTGCCAGTCAGGTTGTCTTCGTCAGCACCAGACAGGA O09ig75 PCA assembly of {?OprF AtD?} (B09ig57) GGCAACTTCGGTGGTAACTCTTTCGATATTCTGGTTCCGCAGAAAACCAAAG O09ig76 PCA assembly of {?OprF AtD?} (B09ig57) GATGTCGTATTTGATACCAACAGAAACGTTGTCTTTGGTTTTCTGCGGAACC O09ig77 PCA assembly of {?OprF AtD?} (B09ig57) TTGGTATCAAATACGACATCGAACTGGAAACTGGCGTTATGCTGGGTGCTAA O09ig78 PCA assembly of {?OprF AtD?} (B09ig57) CGTTAGGATCCGTAAGAAACTTTAGCACCCAGCATAACGCC
Gene Synthesis of B09ig79 {?cl02365 AtD?}
Pool O09ig79 through O09ig112, assemble by PCA PCR O09ig79/O09ig112 on PCA reaction (854, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig79 {?cl02365 AtD?} ---- O09ig79 PCA assembly of {?cl02365 AtD?} (B09ig79) CCATAGAATTCATGAGATCTGAAAACTCTAACGCTCGTA O09ig80 PCA assembly of {?cl02365 AtD?} (B09ig79) CACTGACCAACGCCAGTCATCCACATACGAGCGTTAGAGTTTTCA O09ig81 PCA assembly of {?cl02365 AtD?} (B09ig79) ATGACTGGCGTTGGTCAGTGGGGTAACCTGGACTACGGTTACTCT O09ig82 PCA assembly of {?cl02365 AtD?} (B09ig79) CAGCGTAGAAGTCAACGTCCATGTTAGAGTAACCGTAGTCCAGGT O09ig83 PCA assembly of {?cl02365 AtD?} (B09ig79) GGACGTTGACTTCTACGCTGGTCTGGTTGGTGCTGAAATCGACGT O09ig84 PCA assembly of {?cl02365 AtD?} (B09ig79) CAGACCAGCTTTGGTGTTTTCGGTAACGTCGATTTCAGCACCAAC O09ig85 PCA assembly of {?cl02365 AtD?} (B09ig79) AAAACACCAAAGCTGGTCTGTTCTTCGGTGCTGGTTCTACCCACT O09ig86 PCA assembly of {?cl02365 AtD?} (B09ig79) ATTTTACCGTATTCGTGAGCTTTGTAGTGGGTAGAACCAGCACCG O09ig87 PCA assembly of {?cl02365 AtD?} (B09ig79) GCTCACGAATACGGTAAAATCGACTCTAACGACCTCCATGTTGGT O09ig88 PCA assembly of {?cl02365 AtD?} (B09ig79) CTTTGATGTTAGAAACACCGTACAGACCAACATGGAGGTCGTTAG O09ig89 PCA assembly of {?cl02365 AtD?} (B09ig79) CGGTGTTTCTAACATCAAAGACGTTGCTGCTGTTAACTACGGTAT O09ig90 PCA assembly of {?cl02365 AtD?} (B09ig79) GTCACGGTCCTGGTTGGTGTGGGTGATACCGTAGTTAACAGCAGC O09ig91 PCA assembly of {?cl02365 AtD?} (B09ig79) ACACCAACCAGGACCGTGACGCTAAACGTACCCTGTGGGTTGGTC O09ig92 PCA assembly of {?cl02365 AtD?} (B09ig79) GAGGTAGAGTTGTAACCAGAGGTCTGACCAACCCACAGGGTACGT O09ig93 PCA assembly of {?cl02365 AtD?} (B09ig79) TCTGGTTACAACTCTACCTCTTACGACGCTAAAATCACCCAGTTC O09ig94 PCA assembly of {?cl02365 AtD?} (B09ig79) GTTGGGTGTAAGCACCTTCCAGGAAGAACTGGGTGATTTTAGCGT O09ig95 PCA assembly of {?cl02365 AtD?} (B09ig79) GGAAGGTGCTTACACCCAACTCAACAACGACAAATACTCTATCGA O09ig96 PCA assembly of {?cl02365 AtD?} (B09ig79) GAGCCAGTTGAAACCAGCGTACGGTTCGATAGAGTATTTGTCGTT O09ig97 PCA assembly of {?cl02365 AtD?} (B09ig79) ACGCTGGTTTCAACTGGCTCCATGTTTCTACCGACTCTATCAACG O09ig98 PCA assembly of {?cl02365 AtD?} (B09ig79) GAGAACTGCATGTTACCAACCTGTTCGTTGATAGAGTCGGTAGAA O09ig99 PCA assembly of {?cl02365 AtD?} (B09ig79) GTTGGTAACATGCAGTTCTCTACCAAAACCGACAACCAGGACATC O09ig100 PCA assembly of {?cl02365 AtD?} (B09ig79) CACGCAGACCGATAGTGCCAACCTGGATGTCCTGGTTGTCGGTTT O09ig101 PCA assembly of {?cl02365 AtD?} (B09ig79) TGGCACTATCGGTCTGCGTGGTGGTTACCCGTTCACCGCTGGTTC O09ig102 PCA assembly of {?cl02365 AtD?} (B09ig79) GTCACCTTTCAGAGCCATGTTGATAGAACCAGCGGTGAACGGGTA O09ig103 PCA assembly of {?cl02365 AtD?} (B09ig79) ACATGGCTCTGAAAGGTGACGTTTCTGCTTCTCACCTGTTCGGTG O09ig104 PCA assembly of {?cl02365 AtD?} (B09ig79) TAGAGTTGAGCTTCCGGACGGTTGTCACCGAACAGGTGAGAAGCA O09ig105 PCA assembly of {?cl02365 AtD?} (B09ig79) CGTCCGGAAGCTCAACTCTACCTGTCTAACTCTGGTGAAGCTACC O09ig106 PCA assembly of {?cl02365 AtD?} (B09ig79) GGTTGTCCAGTTTACCACCACGCAGGGTAGCTTCACCAGAGTTAG O09ig107 PCA assembly of {?cl02365 AtD?} (B09ig79) TGGTGGTAAACTGGACAACCTGTTCGGTGTTGGTCTGGGTGTTGA O09ig108 PCA assembly of {?cl02365 AtD?} (B09ig79) GGTGGTAGATTTGTTGAGTTGAGCGTCAACACCCAGACCAACACC O09ig109 PCA assembly of {?cl02365 AtD?} (B09ig79) AACTCAACAAATCTACCACCTTCGGTGTTTCTTACCAGGGTCAGT O09ig110 PCA assembly of {?cl02365 AtD?} (B09ig79) CCAGAAGAAGAAACGTCAGAGTTGTACTGACCCTGGTAAGAAACA O09ig111 PCA assembly of {?cl02365 AtD?} (B09ig79) TCTGACGTTTCTTCTTCTGGTATCAACGCTACCCTGAAAGTTAAC O09ig112 PCA assembly of {?cl02365 AtD?} (B09ig79) CGTTAGGATCCGAAGTTAACTTTCAGGGTAGCGT
Gene Synthesis of B09ig113 {?VtaA11 AtD?}
Pool O09ig113 through O09ig120, assemble by PCA PCR O09ig113/O09ig120 on PCA reaction (269, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig113 {?VtaA11 AtD?} ---- O09ig113 PCA assembly of {?VtaA11 AtD?} (B09ig113) CCATAGAATTCATGAGATCTAACAACAAAGCTCTGCGTGCTGGTATCG O09ig114 PCA assembly of {?VtaA11 AtD?} (B09ig113) CCTGCGGCAGACCAGCAGCAGCGTTAGAACCAGCGATACCAGCACGCAGAGCT O09ig115 PCA assembly of {?VtaA11 AtD?} (B09ig113) TGCTGCTGGTCTGCCGCAGGTTTATATTCCGGGTAAATCTATGGTTGCTGTTT O09ig116 PCA assembly of {?VtaA11 AtD?} (B09ig113) CCAGAGCAGACTGACCTTTGAAAGTGCCAGCAGAAACAGCAACCATAGATTTA O09ig117 PCA assembly of {?VtaA11 AtD?} (B09ig113) CAAAGGTCAGTCTGCTCTGGCTGTTGGTTACTCTCGTGCTTCTGACAACGGTA O09ig118 PCA assembly of {?VtaA11 AtD?} (B09ig113) TGTTAGCGTTACCTTGGAGTTTCAGGATCAGTTTACCGTTGTCAGAAGCACGA O09ig119 PCA assembly of {?VtaA11 AtD?} (B09ig113) ACTCCAAGGTAACGCTAACACCTCTGGTGAAATGGGTGGTTCTGTTGGTGTTG O09ig120 PCA assembly of {?VtaA11 AtD?} (B09ig113) CGTTAGGATCCCCACTGGTAACCAACACCAACAGAACCACCC
Gene Synthesis of B09ig121 {?Hag AtD?}
Pool O09ig121 through O09ig128, assemble by PCA PCR O09ig121/O09ig128 on PCA reaction (269, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig121 {?Hag AtD?} ---- O09ig121 PCA assembly of {?Hag AtD?} (B09ig121) CCATAGAATTCATGAGATCTAACGAAAACAAAGCTAACGCTGGTATCT O09ig122 PCA assembly of {?Hag AtD?} (B09ig121) CCTGCGGCATAGAAGCCATAGCCATAGCAGAAGAGATACCAGCGTTAGCTTTG O09ig123 PCA assembly of {?Hag AtD?} (B09ig121) TATGGCTTCTATGCCGCAGGCTTATATTCCGGGTCGTTCTATGGTTACTGGCG O09ig124 PCA assembly of {?Hag AtD?} (B09ig121) CAACAGCACCCTGACCGTTGTGGGTAGCGATACCGCCAGTAACCATAGAACGA O09ig125 PCA assembly of {?Hag AtD?} (B09ig121) CAACGGTCAGGGTGCTGTTGCTGTTGGTCTGTCTAAACTGTCTGACAACGGTC O09ig126 PCA assembly of {?Hag AtD?} (B09ig121) TGTCAGCAGAACCGTTGATTTTGAAAACCCACTGACCGTTGTCAGACAGTTTA O09ig127 PCA assembly of {?Hag AtD?} (B09ig121) AATCAACGGTTCTGCTGACACTCAAGGCCATGTAGGTGCTGCTGTTGGTGCTG O09ig128 PCA assembly of {?Hag AtD?} (B09ig121) CGTTAGGATCCGAAGTGGAAACCAGCACCAACAGCAGCACCT
Gene Synthesis of B09ig129 {?Pcryo_1225 AtD?}
Pool O09ig129 through O09ig136, assemble by PCA PCR O09ig129/O09ig136 on PCA reaction (272, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig129 {?Pcryo_1225 AtD?} ---- O09ig129 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) CCATAGAATTCATGAGATCTGTTGAAGACGACGCTAACGCTGGT O09ig130 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) GCGGCAGAGAAGACATAGCCATAGCAGCAGAGATACCAGCGTTAGCGTCGTCTT O09ig131 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) GGCTATGTCTTCTCTGCCGCAGGCTTACATCATCGGTAAACCGATGATCGGTGG O09ig132 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) AACAGCAGATTCACCGTTGTAGGTAGCGATACCACCACCGATCATCGGTTTACC O09ig133 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) ACAACGGTGAATCTGCTGTTGCTATCGGTTTCTCTAAAATGTCTAACGACGGTC O09ig134 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) GTGTCAGCAGTGCCGTTCAGTTTCATAACCCAACGACCGTCGTTAGACATTTTA O09ig135 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) CTGAACGGCACTGCTGACACTCAAGGCAATGTAGGTGCTGCTATCGGTGCTGGT O09ig136 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) CGTTAGGATCCGTCGAAGTGGAAACCAGCACCGATAGCAGCAC