Construction Files
From OpenWetWare
Gene Synthesis of B09ig1 {<azo1653 AtD>}
Pool O09ig1 through O09ig26, assemble by PCA PCR O09ig1/O09ig26 on PCA reaction (860, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig1 {<azo1653 AtD>} ---- O09ig1 PCA assembly of {<azo1653 AtD>} (B09ig1) CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC O09ig2 PCA assembly of {<azo1653 AtD>} (B09ig1) TAGCACCAGCGTTACCGTCGTCGTTCATACGCTGAGTGCCAGAAGACATCTGG O09ig3 PCA assembly of {?azo1653 AtD>} (B09ig1) CGACGGTAACGCTGGTGCTACCACCCAGGACTTCGACGGTGCTCTGATCGGTG O09ig4 PCA assembly of {?azo1653 AtD?} (B09ig1) CAACACGCCAGTCACCGAAAGCCGGCAGGTCACCACCGATCAGAGCACCGTCG O09ig5 PCA assembly of {?azo1653 AtD?} (B09ig1) TTTCGGTGACTGGCGTGTTGGTGCTGCTATCGGTTACGACATCGCTCAGGTTT O09ig6 PCA assembly of {?azo1653 AtD?} (B09ig1) TGGTTTCACCCTCTGCCTGACGAGAACCAACAGAAACCTGAGCGATGTCGTAA O09ig7 PCA assembly of {?azo1653 AtD?} (B09ig1) TCAGGCAGAGGGTGAAACCAAAACCCGTCGTTTCGCTCTGTACTCTGGTAAAT O09ig8 PCA assembly of {?azo1653 AtD?} (B09ig1) CACGCAGAGAGTTACCGTCAGCCAGTTCGAAAGATTTACCAGAGTACAGAGCG O09ig9 PCA assembly of {?azo1653 AtD?} (B09ig1) TGACGGTAACTCTCTGCGTGTTTTCGGTGGTGCTGCTTACTCTCTGCACCGTC O09ig10 PCA assembly of {?azo1653 AtD?} (B09ig1) CGTCGATCAGAGAAACGTCACGAGCAGATTCCAGACGGTGCAGAGAGTAAGCA O09ig11 PCA assembly of {?azo1653 AtD?} (B09ig1) TGACGTTTCTCTGATCGACGGTGTTGAACACCTGACCCGTGGTTACGACGTTG O09ig12 PCA assembly of {?azo1653 AtD?} (B09ig1) GGTAAGCCAGTTCACCGAAGAGTTGACGGGTAACAACGTCGTAACCACGGGTC O09ig13 PCA assembly of {?azo1653 AtD?} (B09ig1) CTTCGGTGAACTGGCTTACCGTCTGGGTCTGGGTGAAAAATCTCACGTTGAAC O09ig14 PCA assembly of {?azo1653 AtD?} (B09ig1) CACGCTGAGCAACACGCATCAGACCAACGAACGGTTCAACGTGAGATTTTTCA O09ig15 PCA assembly of {?azo1653 AtD?} (B09ig1) GATGCGTGTTGCTCAGCGTGCTGACGGTTTCCAGGAAGAAGGTGGTTCTGCTG O09ig16 PCA assembly of {?azo1653 AtD?} (B09ig1) GCAGAGCGTTACGCTGAGAGTCACCAGCCAGAGCAGCAGAACCACCTTCTTCC O09ig17 PCA assembly of {?azo1653 AtD?} (B09ig1) CTCTCAGCGTAACGCTCTGCTGATCTCTACCCTGGGTGCTCGTGGTCAGCACG O09ig18 PCA assembly of {?azo1653 AtD?} (B09ig1) TCAGTTCGATGTCCAGACCACCCAGGTTAACAGCGTGCTGACCACGAGCACCC O09ig19 PCA assembly of {?azo1653 AtD?} (B09ig1) TGGTCTGGACATCGAACTGAAAGGTTCTGTTACCTGGCGTCACCTGGAAGGTG O09ig20 PCA assembly of {?azo1653 AtD?} (B09ig1) CAGACAGACGCAGACCCAGTTCCGGACGAACGTCACCTTCCAGGTGACGCCAG O09ig21 PCA assembly of {?azo1653 AtD?} (B09ig1) ACTGGGTCTGCGTCTGTCTGGTGGTGCTCGTTTCACCGTTATGGGCACTGAAC O09ig22 PCA assembly of {?azo1653 AtD?} (B09ig1) CGTTCAGGGTCAGCAGCATAGAGTCACGCGGCAGTTCAGTGCCCATAACGGTG O09ig23 PCA assembly of {?azo1653 AtD?} (B09ig1) TATGCTGCTGACCCTGAACGCTAACTACGACCTGATGGCTGGTGTTGTTCTGA O09ig24 PCA assembly of {?azo1653 AtD?} (B09ig1) CAGAGTCAGAGGTCATAGCGTTAACACCAACGTTCAGAACAACACCAGCCATC O09ig25 PCA assembly of {?azo1653 AtD?} (B09ig1) CGCTATGACCTCTGACTCTGGTAACGCTGCTGCTCTGGTTGCTAACCTGCGCT O09ig26 PCA assembly of {?azo1653 AtD?} (B09ig1) CGTTAGGATCCGTTCATTTTCCAGCGCAGGTTAGCAACCAGA
Gene Synthesis of B09ig27 {<Nmul_A1566 AtD>}
Pool O09ig27 through O09ig56, assemble by PCA PCR O09ig27/O09ig56 on PCA reaction (848, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig27 {?Nmul_A1566 AtD?} ---- O09ig27 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CCATAGAATTCATGAGATCTGCTTTCTGGACCCGTACCTTCGGTTC O09ig28 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GTTCATGTCACCAGAACGGTGACCGAAAGAACCGAAGGTACGGGTCCA O09ig29 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCGTTCTGGTGACATGAACGCTGCTCGTATCAACCGTAACATCGGTG O09ig30 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCAGGGTGTCACCACCCATGAAGAAACCACCGATGTTACGGTTGATA O09ig31 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ATGGGTGGTGACACCCTGGTTGCTGACCTGCTGCGTCTGGGTATCGCT O09ig32 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CGTTGAAAGAAGAGTTAGAGTAACCACCAGCGATACCCAGACGCAGCA O09ig33 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CTCTAACTCTTCTTTCAACGTTAACGAACGTTTCTCTACTGGCTCTTC O09ig34 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCGTAAGCGGTAACGTGGTAGTTGTCAGAAGAGCCAGTAGAGAAACG O09ig35 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCACGTTACCGCTTACGGTGGCACTCGTTGGCACGCTCTGGGTCTGC O09ig36 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TCGTGCCAGGTGTAACCACCACCGAAACGCAGACCCAGAGCGTGCCAA O09ig37 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GGTGGTTACACCTGGCACGACTTCGAAACCAACCGTAATATTCTGTTT O09ig38 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CACCTTTAGCCTGGTTGTTGAAACCTGGAAACAGAATATTACGGTTGG O09ig39 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CAACAACCAGGCTAAAGGTGACTACCGTGGTCGTACTGGCCAGGTTTT O09ig40 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GAACGGCAGTTCGTAACCCAGTTCACCGAAAACCTGGCCAGTACGACC O09ig41 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TGGGTTACGAACTGCCGTTCAAAAACGTTTCTCTGGAACCGTTCGCTG O09ig42 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TTGGTAGCCAGGTTAACGTAAGCAACACCAGCGAACGGTTCCAGAGAA O09ig43 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TACGTTAACCTGGCTACCAAAGGTTTCCAGGAACGTGGTGGTATCGCT O09ig44 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CTTCGTTGTCACGAGAAGAAGACAGAGCAGCGATACCACCACGTTCCT O09ig45 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TTCTTCTCGTGACAACGAAGGTGTTACCTACTCTACCGTTGGTATGAA O09ig46 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCAGCGGTAGAGAACATAGAAGCAGCGTTCATACCAACGGTAGAGTA O09ig47 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CTATGTTCTCTACCGCTGGTGGCACTACCACCCGTCTGCGTGGTTCTC O09ig48 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACAGAGTCGAAAGCGTGACGCCAACCCAGAGAACCACGCAGACGGGTG O09ig49 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CGTCACGCTTTCGACTCTGTTCCGACCCGTTCTTCTGTTGCTTTCTCT O09ig50 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TGCCAGCGATACCGAAAGCAGAACCACCAGAGAAAGCAACAGAAGAAC O09ig51 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TGCTTTCGGTATCGCTGGCACTCCGATCGCTAAAGACGCTATGATCAT O09ig52 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCAACAGAAGCGTCCAGACCAGCACCGATGATCATAGCGTCTTTAGC O09ig53 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GTCTGGACGCTTCTGTTGGTAAAAACGCTATCCTGGGTATCGCTTACA O09ig54 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TCAACAACGTTAGAGAAAACCTGGCCAGTGTAAGCGATACCCAGGATA O09ig55 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GTTTTCTCTAACGTTGTTGACAACGGTGTTCGTGCTAACCTGGACTGG O09ig56 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CGTTAGGATCCGAAACGCCAGTCCAGGTTAGCACGAA
Gene Synthesis of B09ig57 {<OprF AtD>}
Pool O09ig57 through O09ig78, assemble by PCA PCR O09ig57/O09ig78 on PCA reaction (710, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig57 {?OprF AtD?} ---- O09ig57 PCA assembly of {?OprF AtD?} (B09ig57) CCATAGAATTCATGAGATCTGACTCTAAAATGACTGGCGCTTACGGTC O09ig58 PCA assembly of {?OprF AtD?} (B09ig57) AGAGGTAGAGTCAGAGATACCTTTTTCGATCTGACCGTAAGCGCCAGTCATT O09ig59 PCA assembly of {?OprF AtD?} (B09ig57) GTATCTCTGACTCTACCTCTGTTGGTCTGATGCTGGGTGGTAACAACAACAA O09ig60 PCA assembly of {?OprF AtD?} (B09ig57) TACCTTTCAGGTCAGAGGTAGCGATGTCAACTTTGTTGTTGTTACCACCCAG O09ig61 PCA assembly of {?OprF AtD?} (B09ig57) TACCTCTGACCTGAAAGGTAACTCTGCTTACCTGGGTGGTTACGTTAAACAC O09ig62 PCA assembly of {?OprF AtD?} (B09ig57) ATACCAGCGATACCACGGAAAGCACCTTTGTCGTGTTTAACGTAACCACCCA O09ig63 PCA assembly of {?OprF AtD?} (B09ig57) TTCCGTGGTATCGCTGGTATCGGTATCCAGTACTCTGACTGGGACTCTCACC O09ig64 PCA assembly of {?OprF AtD?} (B09ig57) GTTTTCTTTGTAAGAGTCAGACATGGTGTTACGGTGAGAGTCCCAGTCAGAG O09ig65 PCA assembly of {?OprF AtD?} (B09ig57) CTGACTCTTACAAAGAAAACTACTCTGACCGTGGTCTGAACATCTACGCAGA O09ig66 PCA assembly of {?OprF AtD?} (B09ig57) GACCTTCGGTCAGTTCTTTGGTGTATTTACCCTCTGCGTAGATGTTCAGACC O09ig67 PCA assembly of {?OprF AtD?} (B09ig57) CAAAGAACTGACCGAAGGTCTGTTCCTGGAACCGAAAGTTGGTGTTTCTTAC O09ig68 PCA assembly of {?OprF AtD?} (B09ig57) GATTCGTTGATAGATTCCTGTTTGATGTAGTCGTAAGAAACACCAACTTTCG O09ig69 PCA assembly of {?OprF AtD?} (B09ig57) CAGGAATCTATCAACGAATCTAAAGACAAAGCTCTGGCTCTGGAAGTTGACG O09ig70 PCA assembly of {?OprF AtD?} (B09ig57) AACGTTACCAGACAGGGTGTCGAAGTCTTTACCGTCAACTTCCAGAGCCAGA O09ig71 PCA assembly of {?OprF AtD?} (B09ig57) ACACCCTGTCTGGTAACGTTGGTCTGGACCTGCGTAAAGAAATCGTTACCGA O09ig72 PCA assembly of {?OprF AtD?} (B09ig57) TAACACCAGCGGTCAGGTTGTGTTTACCTTTTTCGGTAACGATTTCTTTACG O09ig73 PCA assembly of {?OprF AtD?} (B09ig57) CAACCTGACCGCTGGTGTTAACTACACCCGTATCCTGTCTGGTGCTGACGAA O09ig74 PCA assembly of {?OprF AtD?} (B09ig57) GAGTTACCACCGAAGTTGCCAGTCAGGTTGTCTTCGTCAGCACCAGACAGGA O09ig75 PCA assembly of {?OprF AtD?} (B09ig57) GGCAACTTCGGTGGTAACTCTTTCGATATTCTGGTTCCGCAGAAAACCAAAG O09ig76 PCA assembly of {?OprF AtD?} (B09ig57) GATGTCGTATTTGATACCAACAGAAACGTTGTCTTTGGTTTTCTGCGGAACC O09ig77 PCA assembly of {?OprF AtD?} (B09ig57) TTGGTATCAAATACGACATCGAACTGGAAACTGGCGTTATGCTGGGTGCTAA O09ig78 PCA assembly of {?OprF AtD?} (B09ig57) CGTTAGGATCCGTAAGAAACTTTAGCACCCAGCATAACGCC
Gene Synthesis of B09ig79 {<cl02365 AtD>}
Pool O09ig79 through O09ig112, assemble by PCA PCR O09ig79/O09ig112 on PCA reaction (854, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig79 {?cl02365 AtD?} ---- O09ig79 PCA assembly of {?cl02365 AtD?} (B09ig79) CCATAGAATTCATGAGATCTGAAAACTCTAACGCTCGTA O09ig80 PCA assembly of {?cl02365 AtD?} (B09ig79) CACTGACCAACGCCAGTCATCCACATACGAGCGTTAGAGTTTTCA O09ig81 PCA assembly of {?cl02365 AtD?} (B09ig79) ATGACTGGCGTTGGTCAGTGGGGTAACCTGGACTACGGTTACTCT O09ig82 PCA assembly of {?cl02365 AtD?} (B09ig79) CAGCGTAGAAGTCAACGTCCATGTTAGAGTAACCGTAGTCCAGGT O09ig83 PCA assembly of {?cl02365 AtD?} (B09ig79) GGACGTTGACTTCTACGCTGGTCTGGTTGGTGCTGAAATCGACGT O09ig84 PCA assembly of {?cl02365 AtD?} (B09ig79) CAGACCAGCTTTGGTGTTTTCGGTAACGTCGATTTCAGCACCAAC O09ig85 PCA assembly of {?cl02365 AtD?} (B09ig79) AAAACACCAAAGCTGGTCTGTTCTTCGGTGCTGGTTCTACCCACT O09ig86 PCA assembly of {?cl02365 AtD?} (B09ig79) ATTTTACCGTATTCGTGAGCTTTGTAGTGGGTAGAACCAGCACCG O09ig87 PCA assembly of {?cl02365 AtD?} (B09ig79) GCTCACGAATACGGTAAAATCGACTCTAACGACCTCCATGTTGGT O09ig88 PCA assembly of {?cl02365 AtD?} (B09ig79) CTTTGATGTTAGAAACACCGTACAGACCAACATGGAGGTCGTTAG O09ig89 PCA assembly of {?cl02365 AtD?} (B09ig79) CGGTGTTTCTAACATCAAAGACGTTGCTGCTGTTAACTACGGTAT O09ig90 PCA assembly of {?cl02365 AtD?} (B09ig79) GTCACGGTCCTGGTTGGTGTGGGTGATACCGTAGTTAACAGCAGC O09ig91 PCA assembly of {?cl02365 AtD?} (B09ig79) ACACCAACCAGGACCGTGACGCTAAACGTACCCTGTGGGTTGGTC O09ig92 PCA assembly of {?cl02365 AtD?} (B09ig79) GAGGTAGAGTTGTAACCAGAGGTCTGACCAACCCACAGGGTACGT O09ig93 PCA assembly of {?cl02365 AtD?} (B09ig79) TCTGGTTACAACTCTACCTCTTACGACGCTAAAATCACCCAGTTC O09ig94 PCA assembly of {?cl02365 AtD?} (B09ig79) GTTGGGTGTAAGCACCTTCCAGGAAGAACTGGGTGATTTTAGCGT O09ig95 PCA assembly of {?cl02365 AtD?} (B09ig79) GGAAGGTGCTTACACCCAACTCAACAACGACAAATACTCTATCGA O09ig96 PCA assembly of {?cl02365 AtD?} (B09ig79) GAGCCAGTTGAAACCAGCGTACGGTTCGATAGAGTATTTGTCGTT O09ig97 PCA assembly of {?cl02365 AtD?} (B09ig79) ACGCTGGTTTCAACTGGCTCCATGTTTCTACCGACTCTATCAACG O09ig98 PCA assembly of {?cl02365 AtD?} (B09ig79) GAGAACTGCATGTTACCAACCTGTTCGTTGATAGAGTCGGTAGAA O09ig99 PCA assembly of {?cl02365 AtD?} (B09ig79) GTTGGTAACATGCAGTTCTCTACCAAAACCGACAACCAGGACATC O09ig100 PCA assembly of {?cl02365 AtD?} (B09ig79) CACGCAGACCGATAGTGCCAACCTGGATGTCCTGGTTGTCGGTTT O09ig101 PCA assembly of {?cl02365 AtD?} (B09ig79) TGGCACTATCGGTCTGCGTGGTGGTTACCCGTTCACCGCTGGTTC O09ig102 PCA assembly of {?cl02365 AtD?} (B09ig79) GTCACCTTTCAGAGCCATGTTGATAGAACCAGCGGTGAACGGGTA O09ig103 PCA assembly of {?cl02365 AtD?} (B09ig79) ACATGGCTCTGAAAGGTGACGTTTCTGCTTCTCACCTGTTCGGTG O09ig104 PCA assembly of {?cl02365 AtD?} (B09ig79) TAGAGTTGAGCTTCCGGACGGTTGTCACCGAACAGGTGAGAAGCA O09ig105 PCA assembly of {?cl02365 AtD?} (B09ig79) CGTCCGGAAGCTCAACTCTACCTGTCTAACTCTGGTGAAGCTACC O09ig106 PCA assembly of {?cl02365 AtD?} (B09ig79) GGTTGTCCAGTTTACCACCACGCAGGGTAGCTTCACCAGAGTTAG O09ig107 PCA assembly of {?cl02365 AtD?} (B09ig79) TGGTGGTAAACTGGACAACCTGTTCGGTGTTGGTCTGGGTGTTGA O09ig108 PCA assembly of {?cl02365 AtD?} (B09ig79) GGTGGTAGATTTGTTGAGTTGAGCGTCAACACCCAGACCAACACC O09ig109 PCA assembly of {?cl02365 AtD?} (B09ig79) AACTCAACAAATCTACCACCTTCGGTGTTTCTTACCAGGGTCAGT O09ig110 PCA assembly of {?cl02365 AtD?} (B09ig79) CCAGAAGAAGAAACGTCAGAGTTGTACTGACCCTGGTAAGAAACA O09ig111 PCA assembly of {?cl02365 AtD?} (B09ig79) TCTGACGTTTCTTCTTCTGGTATCAACGCTACCCTGAAAGTTAAC O09ig112 PCA assembly of {?cl02365 AtD?} (B09ig79) CGTTAGGATCCGAAGTTAACTTTCAGGGTAGCGT
Gene Synthesis of B09ig113 {<VtaA11 AtD>}
Pool O09ig113 through O09ig120, assemble by PCA PCR O09ig113/O09ig120 on PCA reaction (269, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig113 {?VtaA11 AtD?} ---- O09ig113 PCA assembly of {?VtaA11 AtD?} (B09ig113) CCATAGAATTCATGAGATCTAACAACAAAGCTCTGCGTGCTGGTATCG O09ig114 PCA assembly of {?VtaA11 AtD?} (B09ig113) CCTGCGGCAGACCAGCAGCAGCGTTAGAACCAGCGATACCAGCACGCAGAGCT O09ig115 PCA assembly of {?VtaA11 AtD?} (B09ig113) TGCTGCTGGTCTGCCGCAGGTTTATATTCCGGGTAAATCTATGGTTGCTGTTT O09ig116 PCA assembly of {?VtaA11 AtD?} (B09ig113) CCAGAGCAGACTGACCTTTGAAAGTGCCAGCAGAAACAGCAACCATAGATTTA O09ig117 PCA assembly of {?VtaA11 AtD?} (B09ig113) CAAAGGTCAGTCTGCTCTGGCTGTTGGTTACTCTCGTGCTTCTGACAACGGTA O09ig118 PCA assembly of {?VtaA11 AtD?} (B09ig113) TGTTAGCGTTACCTTGGAGTTTCAGGATCAGTTTACCGTTGTCAGAAGCACGA O09ig119 PCA assembly of {?VtaA11 AtD?} (B09ig113) ACTCCAAGGTAACGCTAACACCTCTGGTGAAATGGGTGGTTCTGTTGGTGTTG O09ig120 PCA assembly of {?VtaA11 AtD?} (B09ig113) CGTTAGGATCCCCACTGGTAACCAACACCAACAGAACCACCC
Gene Synthesis of B09ig121 {<Hag AtD>}
Pool O09ig121 through O09ig128, assemble by PCA PCR O09ig121/O09ig128 on PCA reaction (269, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig121 {?Hag AtD?} ---- O09ig121 PCA assembly of {?Hag AtD?} (B09ig121) CCATAGAATTCATGAGATCTAACGAAAACAAAGCTAACGCTGGTATCT O09ig122 PCA assembly of {?Hag AtD?} (B09ig121) CCTGCGGCATAGAAGCCATAGCCATAGCAGAAGAGATACCAGCGTTAGCTTTG O09ig123 PCA assembly of {?Hag AtD?} (B09ig121) TATGGCTTCTATGCCGCAGGCTTATATTCCGGGTCGTTCTATGGTTACTGGCG O09ig124 PCA assembly of {?Hag AtD?} (B09ig121) CAACAGCACCCTGACCGTTGTGGGTAGCGATACCGCCAGTAACCATAGAACGA O09ig125 PCA assembly of {?Hag AtD?} (B09ig121) CAACGGTCAGGGTGCTGTTGCTGTTGGTCTGTCTAAACTGTCTGACAACGGTC O09ig126 PCA assembly of {?Hag AtD?} (B09ig121) TGTCAGCAGAACCGTTGATTTTGAAAACCCACTGACCGTTGTCAGACAGTTTA O09ig127 PCA assembly of {?Hag AtD?} (B09ig121) AATCAACGGTTCTGCTGACACTCAAGGCCATGTAGGTGCTGCTGTTGGTGCTG O09ig128 PCA assembly of {?Hag AtD?} (B09ig121) CGTTAGGATCCGAAGTGGAAACCAGCACCAACAGCAGCACCT
Gene Synthesis of B09ig129 {<Pcryo_1225 AtD>}
Pool O09ig129 through O09ig136, assemble by PCA PCR O09ig129/O09ig136 on PCA reaction (272, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig129 {?Pcryo_1225 AtD?} ---- O09ig129 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) CCATAGAATTCATGAGATCTGTTGAAGACGACGCTAACGCTGGT O09ig130 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) GCGGCAGAGAAGACATAGCCATAGCAGCAGAGATACCAGCGTTAGCGTCGTCTT O09ig131 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) GGCTATGTCTTCTCTGCCGCAGGCTTACATCATCGGTAAACCGATGATCGGTGG O09ig132 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) AACAGCAGATTCACCGTTGTAGGTAGCGATACCACCACCGATCATCGGTTTACC O09ig133 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) ACAACGGTGAATCTGCTGTTGCTATCGGTTTCTCTAAAATGTCTAACGACGGTC O09ig134 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) GTGTCAGCAGTGCCGTTCAGTTTCATAACCCAACGACCGTCGTTAGACATTTTA O09ig135 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) CTGAACGGCACTGCTGACACTCAAGGCAATGTAGGTGCTGCTATCGGTGCTGGT O09ig136 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) CGTTAGGATCCGTCGAAGTGGAAACCAGCACCGATAGCAGCAC
Gene Synthesis of B09ig171 {<gliadin binding scFv>}
Pool O09ig171 through O09ig200, assemble by PCA PCR O09ig171/O09ig200 on PCA reaction (842, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig171 {?gliadin binding scFv?} ---- O09ig171 PCA assembly of {?gliadin binding scFv?} (B09ig171) CCATAGAATTCATGAGATCTGCTCAGGTTCAACTCGTTCA O09ig172 PCA assembly of {?gliadin binding scFv?} (B09ig171) ACCCGGCTGAACAACACCACCACCAGACTGAACGAGTTGAACCTGAGC O09ig173 PCA assembly of {?gliadin binding scFv?} (B09ig171) GTGGTGTTGTTCAGCCGGGTCGTTCTCTGCGTCTGTCTTGCGCTGCTT O09ig174 PCA assembly of {?gliadin binding scFv?} (B09ig171) ATATCGTAGATAGAGAAAGAGAAACCAGAAGCAGCGCAAGACAGACGC O09ig175 PCA assembly of {?gliadin binding scFv?} (B09ig171) TCTTTCTCTATCTACGATATGTACTGGGTTCGTCAGGCTCCGGGTAAA O09ig176 PCA assembly of {?gliadin binding scFv?} (B09ig171) TAGAGATAACAGCAACCCATTCCAGACCTTTACCCGGAGCCTGACGAA O09ig177 PCA assembly of {?gliadin binding scFv?} (B09ig171) ATGGGTTGCTGTTATCTCTACCGACGGTTCTAACAAATTCTACGCTGA O09ig178 PCA assembly of {?gliadin binding scFv?} (B09ig171) AGAGATGGTGAAACGACCTTTAACAGAGTCAGCGTAGAATTTGTTAGA O09ig179 PCA assembly of {?gliadin binding scFv?} (B09ig171) AAGGTCGTTTCACCATCTCTCGTGACAACTCTAAAAACACCCTGTACC O09ig180 PCA assembly of {?gliadin binding scFv?} (B09ig171) TCCTCAACACCCAGAGAGTTCATTTGGAGGTACAGGGTGTTTTTAGAG O09ig181 PCA assembly of {?gliadin binding scFv?} (B09ig171) AACTCTCTGGGTGTTGAGGATACCGCTGTTTACTACTGCGCTCGTGAA O09ig182 PCA assembly of {?gliadin binding scFv?} (B09ig171) CACCCCATTCTTCGTAAGAACCGTCGATTTCACGAGCGCAGTAGTAAA O09ig183 PCA assembly of {?gliadin binding scFv?} (B09ig171) TTCTTACGAAGAATGGGGTGACTACTGGGGTCAGGGCACTCTGGTTTC O09ig184 PCA assembly of {?gliadin binding scFv?} (B09ig171) CGGAGAGGTTGGGGAAGCAGAAGAAACAGAAACCAGAGTGCCCTGACC O09ig185 PCA assembly of {?gliadin binding scFv?} (B09ig171) CTGCTTCCCCAACCTCTCCGAAACTGGAAGAAGGTGAGTTTTCCGAGG O09ig186 PCA assembly of {?gliadin binding scFv?} (B09ig171) GGCTGGGTCAGAACAGACTGAACACGAGCCTCGGAAAACTCACCTTCT O09ig187 PCA assembly of {?gliadin binding scFv?} (B09ig171) CAGTCTGTTCTGACCCAGCCGCCGTCTGTTTCTGGTGCTCCGGGTCAG O09ig188 PCA assembly of {?gliadin binding scFv?} (B09ig171) AAGAGCCAGTGCAAGAGATGGTAACACGCTGACCCGGAGCACCAGAAA O09ig189 PCA assembly of {?gliadin binding scFv?} (B09ig171) CATCTCTTGCACTGGCTCTTCTTCTAACATCGGTGCTGGTTACGACGT O09ig190 PCA assembly of {?gliadin binding scFv?} (B09ig171) AGTGCCCGGGAGATGTTGATACCAGTGAACGTCGTAACCAGCACCGAT O09ig191 PCA assembly of {?gliadin binding scFv?} (B09ig171) ATCAACATCTCCCGGGCACTGCTCCGAAACTGCTGATCTACGGTAACA O09ig192 PCA assembly of {?gliadin binding scFv?} (B09ig171) CGGTCCGGAACACCAGACGGACGGTTTTTGTTACCGTAGATCAGCAGT O09ig193 PCA assembly of {?gliadin binding scFv?} (B09ig171) CCGTCTGGTGTTCCGGACCGTTTCTCTGGTTCTAAATCTGGCACTTCT O09ig194 PCA assembly of {?gliadin binding scFv?} (B09ig171) CTTGGAGGCCAGTGATAGCCAGAGAAGCAGAAGTGCCAGATTTAGAAC O09ig195 PCA assembly of {?gliadin binding scFv?} (B09ig171) GGCTATCACTGGCCTCCAAGCTGAGGATGAAGCTGACTACTACTGCGC O09ig196 PCA assembly of {?gliadin binding scFv?} (B09ig171) CCAAACGTTCAGAGAGTCGTCCCAAGCAGCGCAGTAGTAGTCAGCTTC O09ig197 PCA assembly of {?gliadin binding scFv?} (B09ig171) ACGACTCTCTGAACGTTTGGGTTTTCGGTGGTGGCACTAAACTGACCG O09ig198 PCA assembly of {?gliadin binding scFv?} (B09ig171) GACGGAGCAGCTTTCGGCTGACCCAGAACGGTCAGTTTAGTGCCACCA O09ig199 PCA assembly of {?gliadin binding scFv?} (B09ig171) CAGCCGAAAGCTGCTCCGTCTGTTACCCTGTTCCCGCCATCCTCTGCT O09ig200 PCA assembly of {?gliadin binding scFv?} (B09ig171) CGTTAGGATCCAGCAGCAGCAGAGGATGGCGGGAACA
Gene Synthesis of B09ig201 {<Cub>}
Pool O09ig201 through O09ig206, assemble by PCA PCR O09ig201/O09ig206 on PCA reaction (164, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig201 {?Cub?} ---- O09ig201 PCA assembly of {?Cub?} (B09ig201) CCATAGAATTCATGAGATCTTCTGGTGGTATCCCGCCGG O09ig202 PCA assembly of {?Cub?} (B09ig201) TACCAGCGAAGATGAGGCGTTGCTGGTCCGGCGGGATACCACCAGAA O09ig203 PCA assembly of {?Cub?} (B09ig201) ACGCCTCATCTTCGCTGGTAAACAACTCGAAGACGGTCGTACCCTGT O09ig204 PCA assembly of {?Cub?} (B09ig201) TAGATTCTTTCTGAATATTGTAGTCAGACAGGGTACGACCGTCTTCG O09ig205 PCA assembly of {?Cub?} (B09ig201) CAATATTCAGAAAGAATCTACCCTCCATCTGGTTCTGCGTCTGCGTG O09ig206 PCA assembly of {?Cub?} (B09ig201) CGTTAGGATCCACCACCACGCAGACGCAGAACCAGA
Gene Synthesis of B09ig207 {<Nub>}
Pool O09ig207 through O09ig212, assemble by PCA PCR O09ig207/O09ig212 on PCA reaction (173, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig207 {?Nub?} ---- O09ig207 PCA assembly of {?Nub?} (B09ig207) CCATAGAATTCATGAGATCTGGTCTGATGCAAATTTTCGTTAA O09ig208 PCA assembly of {?Nub?} (B09ig207) CAGGGTGCCAGTTTTGCCAGTCAGGGTTTTAACGAAAATTTGCATCAG O09ig209 PCA assembly of {?Nub?} (B09ig207) CTGGCAAAACTGGCACCCTGGAAGTTGAATCTTCTGACACCATCGACA O09ig210 PCA assembly of {?Nub?} (B09ig207) TCTTTGTCCTGGATTTTAGATTTAACGTTGTCGATGGTGTCAGAAGAT O09ig211 PCA assembly of {?Nub?} (B09ig207) TCTAAAATCCAGGACAAAGAAGGTATTCCAGGTGGTTGCCGTCCGCGT O09ig212 PCA assembly of {?Nub?} (B09ig207) CGTTAGGATCCCATAACACGCGGACGGCAACCACCTG
Gene Synthesis of B09ig137 {<Hia AtD>}
Pool O09ig137 through O09ig144, assemble by PCA PCR O09ig137/O09ig144 on PCA reaction (269, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig137 {?Hia AtD?} ---- O09ig137 PCA assembly of {?Hia AtD?} (B09ig137) CCATAGAATTCATGAGATCTCTGCGTCGTGAAACCTCTGGTGGTGTTG O09ig138 PCA assembly of {?Hia AtD?} (B09ig137) CCTGCGGCAGGTTAGCGGTAGCGATAGCAGCAGCAACACCACCAGAGGTTTCA O09ig139 PCA assembly of {?Hia AtD?} (B09ig137) TACCGCTAACCTGCCGCAGGCTTACGTTCAGGGTCGTGGTATGACCTCTGTTG O09ig140 PCA assembly of {?Hia AtD?} (B09ig137) CGATAGCAGACTGACCCTGGTAAGAAGAAACACCAACAGAGGTCATACCACGA O09ig141 PCA assembly of {?Hia AtD?} (B09ig137) CCAGGGTCAGTCTGCTATCGCTGTTGGTGTTTCTGCTGTTTCTGAATCTGGTC O09ig142 PCA assembly of {?Hia AtD?} (B09ig137) TGTTAGCAGAACCAGAGAATTTGAAAACCCAGTGACCAGATTCAGAAACAGCA O09ig143 PCA assembly of {?Hia AtD?} (B09ig137) ATTCTCTGGTTCTGCTAACACCCGTTCTCACGTTGGTGTTGGTGCTGGTGTTG O09ig144 PCA assembly of {?Hia AtD?} (B09ig137) CGTTAGGATCCCCATTGATAGCCAACACCAGCACCAACACCA
Gene Synthesis of B09ig145 {<type IIIs Needle Complex scFV>}
Pool O09ig145 through O09ig170, assemble by PCA PCR O09ig145/O09ig170 on PCA reaction (863, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig145 {?type IIIs Needle Complex scFV?} ---- O09ig145 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) CCATAGAATTCATGAGATCTGCTGAACTGGTTATGACCCAGACCCCGT O09ig146 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) TAACAGTGCCACCAACAGCAGCGGAAACCGGAGACGGGGTCTGGGTCATAACC O09ig147 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) TGCTGTTGGTGGCACTGTTACCATCTCTTGCCAATCCTCTCAGTCTGTTTACA O09ig148 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) GTTTCTGCTGATACCAAGACAGCAGGTTGTTGTTGTAAACAGACTGAGAGGAT O09ig149 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) GTCTTGGTATCAGCAGAAACCGGGTCAGCCGCCGAAACTGCTGATCTACAACG O09ig150 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) AACGAGACGGAACACCAGAAGCCAGTTTAGAAGCGTTGTAGATCAGCAGTTTC O09ig151 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) TTCTGGTGTTCCGTCTCGTTTCAAAGGTTCTGGTTCTGGCACTCAGTTCACCC O09ig152 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) CTGCGTCGTCGCATTCGAGATCGGAAATAGTCAGGGTGAACTGAGTGCCAGAA O09ig153 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) TCTCGAATGCGACGACGCAGCAACCTACTACTGCCAGGGCACTTACTACTCTT O09ig154 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) TAGTGCCACCACCGAAGGTCAGATACCAACCAGAAGAGTAGTAAGTGCCCTGG O09ig155 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) GACCTTCGGTGGTGGCACTAAACTGGACGTTAAAGGTCAGTCTGGTCAGTCTG O09ig156 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) CAACACCCTGACCTTCACCAGAACCCGGTTTACCAGACTGACCAGACTGACCT O09ig157 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) TGGTGAAGGTCAGGGTGTTGGTTCTCAGCAACAACTCGTTGAATCTGGTGGTC O09ig158 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) GCAGGGTCAGCGGAGTGCCTGGAGTAACCAGACGACCACCAGATTCAACGAGT O09ig159 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) AGGCACTCCGCTGACCCTGCGTTGCACCGCTTCTGGTTTCTCTCTGTCTAACT O09ig160 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) TACCCGGAGCCTGACGAACCCAGTTCATGTAGTAGTTAGACAGAGAGAAACCA O09ig161 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) GGTTCGTCAGGCTCCGGGTAAAGGTCTGGAATGGATCGGTATCATCTACGAAT O09ig162 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) CTTTAGCCCAAGAAGCGTAGTAGGTGAAACCAGATTCGTAGATGATACCGATC O09ig163 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) CTACGCTTCTTGGGCTAAAGGTCGTTTCACCATCTCTAAAACCTCTACCACTA O09ig164 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) CCTCGGTGGTTGGGGAGGTGATTTTCAGGTCCATAGTGGTAGAGGTTTTAGAG O09ig165 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) CACCTCCCCAACCACCGAGGATACCGCTACCTACTTCTGCGCTCGTTTCGACA O09ig166 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) CCCACGGGTCGAAAGCACCACCACGGTAACCGATGTCGAAACGAGCGCAGAAG O09ig167 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) TGGTGCTTTCGACCCGTGGGGTCCGGGCACTCTGGTTACCGTTTCTTCTGGTC O09ig168 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) CAACGAGTTGAGAAACAGACGGAGCTTTCGGCTGACCAGAAGAAACGGTAACC O09ig169 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) GTCTGTTTCTCAACTCGTTGGTGCTGAACAAAAGCTCATCTCCGAAGAGGACC O09ig170 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145) CGTTAGGATCCAGCACCGTTCAGGTCCTCTTCGGAGATGAGC