Corum:Protocols

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m (New page: ==Overview== Protocols for the Noireaux Lab at the [http://www.umn.edu University of Minnesota] [Physics Department http://www.physics.umn.edu]. ==Protocols== # Cell Plating # Minicultu...)
m (Protocols)
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# [http://openwetware.org/wiki/Corum:Gel_Purification Gel Extraction of DNA]
# [http://openwetware.org/wiki/Corum:Gel_Purification Gel Extraction of DNA]
# DNA Quantification by Quantifluore
# DNA Quantification by Quantifluore
 +
# DNA Linker Design
# DNA Digestion by Restriction Enzymes
# DNA Digestion by Restriction Enzymes
# [http://openwetware.org/wiki/Corum:DNA_Hybridization Linker Construction by ssDNA Hybridization]
# [http://openwetware.org/wiki/Corum:DNA_Hybridization Linker Construction by ssDNA Hybridization]
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# [http://openwetware.org/wiki/Corum:PCR Polymerase Chain Reaction (PCR)]
# [http://openwetware.org/wiki/Corum:PCR Polymerase Chain Reaction (PCR)]
# Whole Plasmid PCR
# Whole Plasmid PCR
 +
# [http://openwetware.org/wiki/Corum:DNA_Sequencing DNA Sequencing]
# Cell Free Expression in Batch Mode
# Cell Free Expression in Batch Mode
# Cell Free Expression in Continuous Mode
# Cell Free Expression in Continuous Mode

Revision as of 15:33, 25 June 2012

Contents

Overview

Protocols for the Noireaux Lab at the University of Minnesota [Physics Department http://www.physics.umn.edu].

Protocols

  1. Cell Plating
  2. Miniculture
  3. Competent Cells
  4. Transformation
  5. Gel electrophoresis
  6. Miniprep
  7. DNA Purification by PCR Purification Kit
  8. Gel Extraction of DNA
  9. DNA Quantification by Quantifluore
  10. DNA Linker Design
  11. DNA Digestion by Restriction Enzymes
  12. Linker Construction by ssDNA Hybridization
  13. DNA Ligation by T4 Ligase
  14. Polymerase Chain Reaction (PCR)
  15. Whole Plasmid PCR
  16. DNA Sequencing
  17. Cell Free Expression in Batch Mode
  18. Cell Free Expression in Continuous Mode
  19. Cell Free Expression in Phospholipid Vesicles

Contact

or instead, discuss this protocol.

Digital Signature

SC 16:08, 25 June 2012 (EDT)

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