Counter-selectable markers: Difference between revisions

Latest revision as of 09:36, 13 July 2007

gene(selection condition)

lacY (t-o-nitrophenyl-B-D-galactopyranoside)
thyA (trimethoprim)
pheS (p-chlorophenylalanine) see PMID 8125286
- Relaxed substrate specificity mutant of phenylalanine tRNA transferase that will incorporate p-chlorophenylalanine into proteins, killing the cell
?(phage)
- probably >1 gene can confer resistance?
- previous work (Novick/Szilard) has mapped mutation rates confering T1 resistance in chemostats.
pyrF (5FoA)
- Jeff has ordered synthesized/BB'd
- in pyrF- strains can be selected on medium w/o Uracil, so can be selected and coutner-selected.
sacB
tet? - see http://www.openwetware.org/wiki/Tetracycline
UPRT
others???

@@ Line 1: / Line 1: @@
-==Desired Characteristics==
-#Short sequence
-#*shorter markers will enable cheaper synthesis of codon variants with different stabilities.
-#Single gene responsible for selection
-#*enables easier determination of mutations responsible for phenotype
-#*for instance, a system where a consitive repressor turned off a toxin could accumulate mutations in either the repressor or the toxin.
-#Strong selective pressure
-#*less false positives
-#On the chromosome?
-#*Not sure how mutations on a plasmid differ...
-==Candidates==
 ''gene(selection condition)''
 #lacY (t-o-nitrophenyl-B-D-galactopyranoside)
 #thyA (trimethoprim)
-#pheS (p-chrlorophenylalanine)
+#pheS (p-chlorophenylalanine) see PMID 8125286
+#*Relaxed substrate specificity mutant of phenylalanine tRNA transferase that will incorporate p-chlorophenylalanine into proteins, killing the cell
 #?(phage)
 #*probably >1 gene can confer resistance?
@@ Line 23: / Line 11: @@
 #*in pyrF- strains can be selected on medium w/o Uracil, so can be selected and coutner-selected.
 #sacB
-#tet?
+#tet? - see http://www.openwetware.org/wiki/Tetracycline
+#UPRT
 #others???