Create running gel

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Current revision (13:40, 6 September 2006) (view source)
 
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To create 10ml of Running gel
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{| border="1"
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|+ To create 10ml of Running gel
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! Material !! 7.5% !! 9% !! 10% !! 11% !! 12.5%
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|-
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! 30% Acrylamide
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| 2.5 || 3 || 3.3 || 3.6 || 4.2
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|-
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! 1.5M Tris pH8.8
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| 2.5 || 2.5 || 2.5 || 2.5 || 2.5
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|-
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! 10%SDS
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| 100ul || 100ul || 100ul || 100ul || 100ul
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|-
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! 10%APS
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| 100ul || 100ul || 100ul || 100ul || 100ul
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|-
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! TEMED
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| 10ul || 10ul || 10ul || 10ul || 10ul
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|-
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! Water
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| 4.8 || 4.3 || 4 || 3.7 || 3.1
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|-
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|}

Current revision

To create 10ml of Running gel
Material 7.5% 9% 10% 11% 12.5%
30% Acrylamide 2.5 3 3.3 3.6 4.2
1.5M Tris pH8.8 2.5 2.5 2.5 2.5 2.5
10%SDS 100ul 100ul 100ul 100ul 100ul
10%APS 100ul 100ul 100ul 100ul 100ul
TEMED 10ul 10ul 10ul 10ul 10ul
Water 4.8 4.3 4 3.7 3.1
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