Cresol Red: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(illustration) |
(molecular weight, pH indicator) |
||
| Line 1: | Line 1: | ||
[[Image:Cresol red.png|right|150px]] | [[Image:Cresol red.png|right|150px]] | ||
'''Cresol red''' is a pH indicator and molecular weight marker for agarose gels. | '''Cresol red''' is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs. | ||
== Agarose molecular weight marker == | |||
advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue | |||
* molecular weight 404 g/mol | |||
* in 1% agarose at ~125 bp | |||
* in 2% agarose at ~300 bp (between xylene cyanol and bromophenol blue) | |||
== pH indicator == | |||
* amber - acidic pH | |||
* yellow - neutral | |||
* red - alkaline | |||
== External links == | == External links == | ||
Revision as of 03:44, 14 January 2009
Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.
Agarose molecular weight marker
advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue
- molecular weight 404 g/mol
- in 1% agarose at ~125 bp
- in 2% agarose at ~300 bp (between xylene cyanol and bromophenol blue)
pH indicator
- amber - acidic pH
- yellow - neutral
- red - alkaline
