Cresol Red: Difference between revisions

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(molecular weight, pH indicator)
m (Cresol red moved to Cresol Red)

Revision as of 03:45, 14 January 2009

Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.

Agarose molecular weight marker

advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue

  • molecular weight 404 g/mol
  • in 1% agarose at ~125 bp
  • in 2% agarose at ~300 bp (between xylene cyanol and bromophenol blue)

pH indicator

  • amber - acidic pH
  • yellow - neutral
  • red - alkaline

External links