Cresol Red

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Agarose molecular weight marker)
(links)
Line 7: Line 7:
* molecular weight 404 g/mol
* molecular weight 404 g/mol
-
* in 1% agarose at ~1000 bp
+
* in 1% agarose at ~1000 bp (between xylene cyanol and bromophenol blue)
== pH indicator ==
== pH indicator ==
Line 16: Line 16:
== External links ==
== External links ==
* [http://www.wendychao.com/science/protocols/loading_buffer/ Wendy Chao's loading dye recipe and discussion]
* [http://www.wendychao.com/science/protocols/loading_buffer/ Wendy Chao's loading dye recipe and discussion]
 +
* [http://molbiol.ru/eng/protocol/01_03.html#a5 Cresol Red solution calculator]
* [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem]
* [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem]
* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=114480|Aldrich&N5=Product%20No.|BRAND_KEY&F=SPEC Cresol Red sodium salt - Sigma catalogue]
* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=114480|Aldrich&N5=Product%20No.|BRAND_KEY&F=SPEC Cresol Red sodium salt - Sigma catalogue]
* [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties]  
* [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties]  
* [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia]
* [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia]

Revision as of 05:59, 14 January 2009

Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.

Agarose molecular weight marker

advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue

  • molecular weight 404 g/mol
  • in 1% agarose at ~1000 bp (between xylene cyanol and bromophenol blue)

pH indicator

  • amber - acidic pH
  • yellow - neutral
  • red - alkaline

External links

Personal tools