Cresol Red

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(Agarose molecular weight marker)
m (External links)
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== External links ==
== External links ==
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* [http://www.wendychao.com/science/protocols/loading_buffer/ Wendy Chao's loading dye recipe and discussion]
+
* [[Image:3stars.png]] [http://www.wendychao.com/science/protocols/loading_buffer/ Wendy Chao's loading dye recipe and discussion]
* [http://molbiol.ru/eng/protocol/01_03.html#a5 Cresol Red solution calculator]
* [http://molbiol.ru/eng/protocol/01_03.html#a5 Cresol Red solution calculator]
* [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem]
* [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem]

Revision as of 06:16, 14 January 2009

Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.

Agarose molecular weight marker

advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue

  • molecular weight 404 g/mol
  • in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp)
  • in 3% agarose at >100bp (xylene cyanol 300bp)

pH indicator

  • amber - acidic pH
  • yellow - neutral
  • red - alkaline

External links

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