Cresol Red: Difference between revisions
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== External links == | == External links == | ||
* [http://www.wendychao.com/science/protocols/loading_buffer/ Wendy Chao's loading dye recipe and discussion] | * [[Image:3stars.png]] [http://www.wendychao.com/science/protocols/loading_buffer/ Wendy Chao's loading dye recipe and discussion] | ||
* [http://molbiol.ru/eng/protocol/01_03.html#a5 Cresol Red solution calculator] | * [http://molbiol.ru/eng/protocol/01_03.html#a5 Cresol Red solution calculator] | ||
* [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem] | * [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem] |
Revision as of 04:16, 14 January 2009
Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.
Agarose molecular weight marker
advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue
- molecular weight 404 g/mol
- in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp)
- in 3% agarose at >100bp (xylene cyanol 300bp)
pH indicator
- amber - acidic pH
- yellow - neutral
- red - alkaline