Cresol Red: Difference between revisions
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[[Image:Cresol red.png|right|150px]] | [[Image:Cresol red.png|right|150px|thumb|chemical structure of cresol red]] | ||
'''Cresol red''' is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs. | [[Image:Agarose gel dyes.jpg|right|150px]] | ||
'''Cresol red''' is a pH indicator and [[Agarose_gel_loading_dye#Colour|molecular weight marker for agarose gels]]. Unlike [[bromophenol blue]] and [[xylene cyanol]], cresol red does not inhibit Taq polymerase in PCRs. | |||
== Agarose molecular weight marker == | == Agarose molecular weight marker == | ||
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* molecular weight 404 g/mol | * molecular weight 404 g/mol | ||
* in 1% agarose at ~1000 bp | * in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp) | ||
* in 3% agarose at >100bp (xylene cyanol 300bp) | |||
== pH indicator == | == pH indicator == | ||
| Line 15: | Line 18: | ||
== External links == | == External links == | ||
* [http://www.wendychao.com/science/protocols/loading_buffer/ Wendy Chao's loading dye recipe and discussion] | * [[Image:3stars.png]] [http://www.wendychao.com/science/protocols/loading_buffer/ Wendy Chao's loading dye recipe and discussion] | ||
* [http://molbiol.ru/eng/protocol/01_03.html#a5 Cresol Red solution calculator] | |||
* [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem] | * [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem] | ||
* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=114480|Aldrich&N5=Product%20No.|BRAND_KEY&F=SPEC Cresol Red sodium salt - Sigma catalogue] | * [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=114480|Aldrich&N5=Product%20No.|BRAND_KEY&F=SPEC Cresol Red sodium salt - Sigma catalogue] | ||
* [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties] | * [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties] | ||
* [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia] | * [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia] | ||
[[Category:Material]] [[Category:Chemical]] [[Category:agarose gel electrophoresis]] | |||
Latest revision as of 07:31, 3 June 2009
Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.
Agarose molecular weight marker
advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue
- molecular weight 404 g/mol
- in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp)
- in 3% agarose at >100bp (xylene cyanol 300bp)
pH indicator
- amber - acidic pH
- yellow - neutral
- red - alkaline
