Cresol Red: Difference between revisions
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[[Image:Cresol red.png|right|150px]] | [[Image:Cresol red.png|right|150px|thumb|chemical structure of cresol red]] | ||
'''Cresol red''' is a pH indicator and [[Agarose_gel_loading_dye#Colour|molecular weight marker for agarose gels]]. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs. | [[Image:Agarose gel dyes.jpg|right|150px]] | ||
'''Cresol red''' is a pH indicator and [[Agarose_gel_loading_dye#Colour|molecular weight marker for agarose gels]]. Unlike [[bromophenol blue]] and [[xylene cyanol]], cresol red does not inhibit Taq polymerase in PCRs. | |||
== Agarose molecular weight marker == | == Agarose molecular weight marker == | ||
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* [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties] | * [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties] | ||
* [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia] | * [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia] | ||
[[Category:Material]] [[Category:Chemical]] [[Category:agarose gel electrophoresis]] | |||
Latest revision as of 07:31, 3 June 2009
Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.
Agarose molecular weight marker
advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue
- molecular weight 404 g/mol
- in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp)
- in 3% agarose at >100bp (xylene cyanol 300bp)
pH indicator
- amber - acidic pH
- yellow - neutral
- red - alkaline
