Cresol Red: Difference between revisions
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[[Image:Cresol red.png|right|150px]] | [[Image:Cresol red.png|right|150px|thumb|chemical structure of cresol red]] | ||
'''Cresol red''' is a pH indicator and [[Agarose_gel_loading_dye#Colour|molecular weight marker for agarose gels]]. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs. | '''Cresol red''' is a pH indicator and [[Agarose_gel_loading_dye#Colour|molecular weight marker for agarose gels]]. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs. | ||
Revision as of 07:29, 3 June 2009
Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.
Agarose molecular weight marker
advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue
- molecular weight 404 g/mol
- in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp)
- in 3% agarose at >100bp (xylene cyanol 300bp)
pH indicator
- amber - acidic pH
- yellow - neutral
- red - alkaline
