Critical micelle concentration (CMC): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(detergent table) |
No edit summary |
||
| Line 10: | Line 10: | ||
! style="background:lightgrey"|Type | ! style="background:lightgrey"|Type | ||
|- | |- | ||
| BRIJ 35 || | | BRIJ 35 || 0.11 || 0.09 || 1200 || non-ionic | ||
|- | |- | ||
| NP-40 || 0.017 || | | NP-40 || 0.017 || 0.3 || ''~650'' || non-ionic | ||
|- | |- | ||
| [[Triton X-100]] || 0.021 || 0.3 || 650 || non-ionic | | [[Triton X-100]] || 0.021 || 0.3 || ''~650'' || non-ionic | ||
|- | |- | ||
| [[Tween 20]] || | | [[Tween 20]] || 0.073 || 0.059 || ''~1228'' || non-ionic | ||
|- | |- | ||
| [[SDS]] || 0.23 || 7-10 || 288.5 || ionic | | [[SDS]] || 0.23 || 7-10 || 288.5 || ionic | ||
| Line 23: | Line 23: | ||
|- | |- | ||
|} | |} | ||
Note: The molecular weights for some detergents are average values. Triton X-100, for example, can range between 600 and over 650 MW depending on synthesis. The exact molecular weight influences the CMC. | |||
== Sources == | == Sources == | ||
* [http://www.emdbiosciences.com/docs/docs/LIT/Detergents_CB0733_E.pdf | * [http://www.emdbiosciences.com/docs/docs/LIT/Detergents_CB0733_E.pdf new, abbreviated Detergent guide, Calbiochem] | ||
* [http://www.med.ufl.edu/biochem/bch6206/DETERGENTS.pdf Commonly used detergents, Frost | * [http://www.med.ufl.edu/biochem/bch6206/DETERGENTS.pdf Commonly used detergents, Frost lecture, UFL] | ||
== See also == | == See also == | ||
* [[ | * [http://en.wikipedia.org/wiki/Critical_micelle_concentration Wikipedia: Critical micelle concentration] | ||
* [http://www.antibodybeyond.com/books/Calbiochem_Detergents_Booklet.pdf old (2001) Calbiochem Detergent brochure] | |||
[[Category:Material]] | [[Category:Material]] | ||
[[Category:Detergents]] | [[Category:Detergents]] | ||
Revision as of 07:22, 28 July 2009
Critical micelle concentration (CMC) is defined as the concentration of detergents above which micelles are spontaneously formed. The CMC is important in biology because at concentrations above it the detergents form complexes with lipophilic proteins. Below this borderline, detergents merely partition into membranes without solubilising membrane proteins.
| Detergent | CMC (%w/v) | CMC (mM) | MW | Type |
|---|---|---|---|---|
| BRIJ 35 | 0.11 | 0.09 | 1200 | non-ionic |
| NP-40 | 0.017 | 0.3 | ~650 | non-ionic |
| Triton X-100 | 0.021 | 0.3 | ~650 | non-ionic |
| Tween 20 | 0.073 | 0.059 | ~1228 | non-ionic |
| SDS | 0.23 | 7-10 | 288.5 | ionic |
| CHAPS | 0.49 | 6-10 | 615 | zwitterionic |
Note: The molecular weights for some detergents are average values. Triton X-100, for example, can range between 600 and over 650 MW depending on synthesis. The exact molecular weight influences the CMC.
