Critical micelle concentration (CMC): Difference between revisions
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| NP-40 || 0.017 || 0.3 || ''~650'' || non-ionic | | NP-40 || 0.017 || 0.3 || ''~650'' || non-ionic | ||
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| Saponin || ~0.1 || || mixture || non-ionic | |||
|- | |- | ||
| [[Triton X-100]] || 0.021 || 0.3 || ''~650'' || non-ionic | | [[Triton X-100]] || 0.021 || 0.3 || ''~650'' || non-ionic | ||
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Note: The molecular weights for some detergents are average values. Triton X-100, for example, can range between 600 and over 650 MW depending on synthesis. The exact molecular weight influences the CMC. | Note: The molecular weights for some detergents are average values. Triton X-100, for example, can range between 600 and over 650 MW depending on synthesis. The exact molecular weight influences the CMC. | ||
Note: [http://en.wikipedia.org/wiki/Saponin Saponin] is a class of amphiphilic, natural metabolites with detergent properties. | |||
== Sources == | == Sources == | ||
* [http://www.emdbiosciences.com/docs/docs/LIT/Detergents_CB0733_E.pdf new, abbreviated Detergent guide, Calbiochem] | * [http://www.emdbiosciences.com/docs/docs/LIT/Detergents_CB0733_E.pdf new, abbreviated Detergent guide, Calbiochem] | ||
* [http://www.med.ufl.edu/biochem/bch6206/DETERGENTS.pdf Commonly used detergents, Frost lecture, UFL] | * [http://www.med.ufl.edu/biochem/bch6206/DETERGENTS.pdf Commonly used detergents, Frost lecture, UFL] | ||
* [http://dx.doi.org/10.1016/j.chemosphere.2004.07.048 Urum et al 2004, incl. data on saponin] | |||
== See also == | == See also == | ||
Revision as of 09:38, 29 July 2009
Critical micelle concentration (CMC) is defined as the concentration of detergents above which micelles are spontaneously formed. The CMC is important in biology because at concentrations above it the detergents form complexes with lipophilic proteins. Below this borderline, detergents merely partition into membranes without solubilising membrane proteins.
| Detergent | CMC (%w/v) | CMC (mM) | MW | Type |
|---|---|---|---|---|
| BRIJ 35 | 0.11 | 0.09 | 1200 | non-ionic |
| NP-40 | 0.017 | 0.3 | ~650 | non-ionic |
| Saponin | ~0.1 | mixture | non-ionic | |
| Triton X-100 | 0.021 | 0.3 | ~650 | non-ionic |
| Tween 20 | 0.073 | 0.059 | ~1228 | non-ionic |
| SDS | 0.23 | 7-10 | 288.5 | ionic |
| CHAPS | 0.49 | 6-10 | 615 | zwitterionic |
Note: The molecular weights for some detergents are average values. Triton X-100, for example, can range between 600 and over 650 MW depending on synthesis. The exact molecular weight influences the CMC.
Note: Saponin is a class of amphiphilic, natural metabolites with detergent properties.
Sources
- new, abbreviated Detergent guide, Calbiochem
- Commonly used detergents, Frost lecture, UFL
- Urum et al 2004, incl. data on saponin
