Cronn Lab:Informatics

From OpenWetWare
Revision as of 19:23, 2 October 2009 by Brian J. Knaus (talk | contribs)
Jump to navigationJump to search

Home        Research        Lab Members        Protocols        Informatics        Calendar        Links       


Solexa Barcode Sorting

Most of our Solexa runs include multiplex massively parallel sequencing (MMPS). Because these micro-reads include a sample-specific barcode (as well as the quality control 'T') a first step is to sort these reads by barcode and to remove the barcode. This is facilitated by a custom perl script.

De Novo Assembly

For de novo assembly of micro-reads we typically use velvet.

Reference Based Assembly

When we have a reasonable reference we use either RGA or MAQ.

Pine2 Software

The lab group is getting a new server named 'pine2.' During the setup process our wonderful sysadmin will be involved with installing software. In order to help get as much software as we would like on the system we're using this space as a list of softwares for him to install. In the interest of stability, he usually uses old versions and does not update them. If you're interested in a particular version please state it.

  • R 2.1.0
    • ape
    • seqinr
    • RMySQL
  • Perl 5.10.1
  • MySQL
  • Emacs
  • Emacs Speaks Statistics
  • Seaview