Cronn Lab:Protocols: Difference between revisions
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== | ==Illumina GA Data Management== | ||
[[Short read toolbox]]. Many of our projects use short-read data from the Illumina Genome Analyzer 1 & 2. [[user: Brian J. Knaus | Brian Knaus]] in our lab has developed a number of scripts for managing and analyzing short-read files and data. | |||
== | ==Illumina GA DNA-Seq== | ||
[[DNA_Seq Prep]]. Our research group has developed several methods for sequencing small genomes (mitochondria, chloroplasts, BACS) in multiplex using Illumina GA2. This page provides details on DNA-Seq library construction. | |||
== | ==Illumina GA RNA-Seq== | ||
[[RNA_Seq Prep]]. We do mRNA-sequencing using methods developed by [http://www.cgrb.oregonstate.edu/faculty/mockler| Todd Mockler's] group at Oregon State University. This page provides details on RNA-Seq library construction. | |||
== | ==Illumina GA Hyb-Seq== | ||
[[Hyb_Seq Prep]]. Like many groups, we've developed customized approaches to enrich rare genomic targets for high-throughput sequencing. Our method for isolating chloroplast genomes by Hyb-Seq is detailed here. | |||
== | ==Random Lab Methods== | ||
== | [[Lab_Methods]]. DNA extraction, RNA extraction, gels, and short cuts... all here | ||
==References on the Illumina GA Sequencer== | |||
*[http://www.illumina.com/downloads/SS_DNAsequencing.pdf Illumina Sequencing Technology] | *[http://www.illumina.com/downloads/SS_DNAsequencing.pdf Illumina Sequencing Technology] | ||
*[http://www.illumina.com/downloads/GenomicSeq_DataSheet.pdf Genomic Sequencing] | *[http://www.illumina.com/downloads/GenomicSeq_DataSheet.pdf Genomic Sequencing] | ||
*[http://www.illumina.com/downloads/targetedresequencing.pdf Targeted Resequencing] | *[http://www.illumina.com/downloads/targetedresequencing.pdf Targeted Resequencing] | ||
Revision as of 01:08, 25 November 2009
Illumina GA Data Management
Short read toolbox. Many of our projects use short-read data from the Illumina Genome Analyzer 1 & 2. Brian Knaus in our lab has developed a number of scripts for managing and analyzing short-read files and data.
Illumina GA DNA-Seq
DNA_Seq Prep. Our research group has developed several methods for sequencing small genomes (mitochondria, chloroplasts, BACS) in multiplex using Illumina GA2. This page provides details on DNA-Seq library construction.
Illumina GA RNA-Seq
RNA_Seq Prep. We do mRNA-sequencing using methods developed by Todd Mockler's group at Oregon State University. This page provides details on RNA-Seq library construction.
Illumina GA Hyb-Seq
Hyb_Seq Prep. Like many groups, we've developed customized approaches to enrich rare genomic targets for high-throughput sequencing. Our method for isolating chloroplast genomes by Hyb-Seq is detailed here.
Random Lab Methods
Lab_Methods. DNA extraction, RNA extraction, gels, and short cuts... all here
