Cronn Lab:Protocols
Illumina GA Data Management
Short read toolbox. Many of our projects use short-read data from the Illumina Genome Analyzer 1 & 2. Brian Knaus in our lab has developed a number of scripts for managing and analyzing short-read files and data.
Illumina GA DNA-Seq
DNA_Seq Prep. Our research group has developed several methods for sequencing small genomes (mitochondria, chloroplasts, BACS) in multiplex using Illumina GA2. This page provides details on DNA-Seq library construction.
Illumina GA RNA-Seq
RNA_Seq Prep. We do mRNA-sequencing using methods developed by Todd Mockler's group at Oregon State University. This page provides details on RNA-Seq library construction.
Illumina GA Hyb-Seq
Hyb_Seq Prep. Like many groups, we've developed customized approaches to enrich rare genomic targets for high-throughput sequencing. Our method for isolating chloroplast genomes by Hyb-Seq is detailed here.
Whole Genome Amplification
WGA Prep. We use phi29-based whole-genome amplification in a variety of different applications. Our standard phi29 WGA method is detailed here.
Random Lab Methods
Random Lab Methods. DNA extraction, RNA extraction, gels, and short cuts... all here
