Cumbers:ideas/FOXO phospholocator

Intro

• FOXO regulates lifespan in the fly
• Acts as a transcription factor when dephosphorylated
• Phosphorylated by AKT

Ideas

• Move FOXO to the nuclues on exogenous signal (yeast pheromone), with Samantha Sutton's phospholocator.
• Look for FOXO in yeast and use the existing system if it exists in yeast Doesn't look like it exists, only other FORKHEADS. anyhow, better to try it in s2 cells.
• Marc Tatar suggests using fly S2 cells (stem cell lines) instead of yeast. using the existing constructs and seeing if they work.

Plan of action

Plan A

• Identify the NLS in FOXO
• Use Samantha's phospholocator (NLS + Fus3) or (Cdc28/Clb2)
• But these might not work in S2 cells, so might need to rethink.

plan B

• Reengineer the AKT phosphorylation site to use the existing FOXO NLS
• much more difficult at this stage.