Cumbers:protocols/picogreen quantIT

• Follow the Quant-it protocol for DNA (and RNA) quantification.
• Make up standard measurements along with your samples.
• If unsure of your scale (if you have a lot or a little) then do 3 concentrations of your sample to check, one at 100% concentration, one at 1:10 other at 1:100
• add 1ul DNA to 9ul TE to make 1:10, then take a ul of this and add 99ul TE to it to make 1:100
• Do 2 of TE or whatever you are resuspending in to subtract this background from your results.
• set up the plate reader and computer.
• Add the fluorescent reagent (make up enough: 100 assays = 20 ml HS buffer) - 200ul added to each plate, make sure you have enough and careful when pipetting, keep track of which hole you want to add it to.
• read the plate

   1   2   3         4  
A  10  10  1C        1S
B  8   8   1C(1:10)  1S(1:10)
C  6   6   1C(1:10)  1S(1:10)
D  4   4   1C(1:10)  1S(1:10)
E  2   2   1C(1:100) 1S(1:100)
F  1   1   1C(1:100) 1S(1:100)
G  0.5 0.5 1C(1:100) 1S(1:100)
H  0   0   TE        TE