Cumbers:protocols/picogreen quantIT
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- Follow the Quant-it protocol for DNA (and RNA) quantification.
- Make up standard measurements along with your samples.
- If unsure of your scale (if you have a lot or a little) then do 2 concentrations of your sample to check, one at 1:10 other at 1:100
- add 1ul DNA to 9ul TE (then take 1ul of this and add 200ul)
- Do 2 of TE or whatever you are resuspending in to subtract this background from your results.
- set up the plate reader and computer.
- Add the fluorescent reagent (make up enough: 100 assays = 20 ml HS buffer) - 200ul added to each plate, make sure you have enough and careful when pipetting, keep track of which hole you want to add it to.
- read the plate
1 2 3 4 A 10 10 1C 1S B 8 8 1C(1:10) 1S(1:10) C 6 6 1C(1:10) 1S(1:10) D 4 4 1C(1:10) 1S(1:10) E 2 2 1C(1:100) 1S(1:100) F 1 1 1C(1:100) 1S(1:100) G 0.5 0.5 1C(1:100) 1S(1:100) H 0 0 TE TE