DIYbio/BOSSlab/Notebook/2011/05/11: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> BOSSLAB</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Preflight== | == Preflight == | ||
=== what's our goal === | === what's our goal === | ||
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* hot water; combusted propane gas | * hot water; combusted propane gas | ||
== Protocol == | |||
# Thaw competent cells (Lyocomp E. coli) on ice. | # Thaw competent cells (Lyocomp E. coli) on ice. | ||
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# Incubate plates at 37°C for 18 h. | # Incubate plates at 37°C for 18 h. | ||
== Visual Protocol == | |||
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<a href="http://www.flickr.com/photos/macowell/5713410863/" title="2011-05-11 visual protocol by macowell, on Flickr"><img src="http://farm4.static.flickr.com/3421/5713410863_fc62439a0e.jpg" width="500" height="374" alt="2011-05-11 visual protocol"></a> | |||
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Latest revision as of 20:23, 26 September 2017
BOSSLAB | Main project page Next entry |
Preflightwhat's our goaldry-run through transformation protocol w/ BBa_J04450 on card using Invivogen lyocomp cells
what are the risks today to us
what are the risks today to the community
what waste are we gonna generate
Protocol
Visual Protocol<html> <a href="http://www.flickr.com/photos/macowell/5713410863/" title="2011-05-11 visual protocol by macowell, on Flickr"><img src="http://farm4.static.flickr.com/3421/5713410863_fc62439a0e.jpg" width="500" height="374" alt="2011-05-11 visual protocol"></a> </html> |