DIYbio/BOSSlab/Notebook/2011/05/11: Difference between revisions
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==Preflight== | == Preflight == | ||
=== what's our goal === | === what's our goal === | ||
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* hot water; combusted propane gas | * hot water; combusted propane gas | ||
== Protocol == | |||
# Thaw competent cells (Lyocomp E. coli) on ice. | # Thaw competent cells (Lyocomp E. coli) on ice. | ||
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# Incubate plates at 37°C for 18 h. | # Incubate plates at 37°C for 18 h. | ||
== Visual Protocol == | |||
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<a href="http://www.flickr.com/photos/macowell/5713410863/" title="2011-05-11 visual protocol by macowell, on Flickr"><img src="http://farm4.static.flickr.com/3421/5713410863_fc62439a0e.jpg" width="500" height="374" alt="2011-05-11 visual protocol"></a> | |||
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Revision as of 11:58, 12 May 2011
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Preflightwhat's our goaldry-run through transformation protocol w/ BBa_J04450 on card using Invivogen lyocomp cells
what are the risks today to us
what are the risks today to the community
what waste are we gonna generate
Protocol
Visual Protocol<html> <a href="http://www.flickr.com/photos/macowell/5713410863/" title="2011-05-11 visual protocol by macowell, on Flickr"><img src="http://farm4.static.flickr.com/3421/5713410863_fc62439a0e.jpg" width="500" height="374" alt="2011-05-11 visual protocol"></a> </html> |