Latest revision as of 21:20, 5 September 2012

Intro In The News Educational Equipment Projects Kits Methods DIYbio.org DIYbio googlegroup FriendFeed - DIYbio http://c.statcounter.com/5195189/0/1a5d59b0/1/0.png

DIYbio FAQ v1.5: "The biohacker's FAQ"

This FAQ for DIYbio is actively maintained by it's editors, and by you! Edit your contributions directly or email updates to the DIYbio email list, diybio@googlegroups.com.

Major contributors (in alphabetical order):

The contents of this FAQ are copyright under the OpenWetWare Copyright policy (Creative Commons Attribution-ShareAlike 3.0 Unported). When quoting any content of this FAQ elsewhere, include a full hypertext link back to the main FAQ page.

This Frequently Asked Questions document is for the DIYBio mailing list. This FAQ is now split into multiple topics for easier reading.

FAQ Revision History

1.0 - copied on 4/7/2009 from heybryan.org...DIYbio_FAQ
1.1 - some updates to clarify original version
1.2 - new sections, reorg, + sections about DIY agar DOI:10.1007/BF00152620 --jcline@ieee.org
1.3 - expand projects sections. Add Laboratory Basics section. --jcline@ieee.org
1.4 - add 'Methods' section, move Laboratory Basics into 'Methods' --jcline@ieee.org
1.5 - Add 'News' section, move news articles there. jcline@ieee.org 23:40, 23 May 2011 (EDT)
1.6 - Multiple updates to project section jcline@ieee.org 00:20, 6 September 2012 (EDT)

What is DIYbio, as an organization?

DIYbio is an organization that aims to help make biology a worthwhile pursuit for citizen scientists, amateur biologists, and DIY biological engineers who value openness and safety. This will require mechanisms for amateurs to increase their knowledge and skills, access to a community of experts, the development of a code of ethics, responsible oversight, and leadership on issues that are unique to doing biology outside of traditional professional settings.

DIYbio is a distributed community of amateur or professional biologists, industry professional or amateur engineers, biomedical engineers, life scientists, computer scientists, etc. Our activities range across a broad spectrum, from molecular naturalism (sequencing part of your own genome or bacterial populations) to biological engineering to building low-cost, open-source alternative lab equipment (Gel Box 2.0) to writing open source software for biology, to creating open source hardware systems and manufacturing.

<html> <div style="float:left;"><object width="560" height="315"><param name="allowfullscreen" value="true" /><param name="allowscriptaccess" value="always" /><param name="movie" value="http://vimeo.com/moogaloop.swf?clip_id=3454392&server=vimeo.com&show_title=1&show_byline=1&show_portrait=0&color=00ADEF&fullscreen=1" /><embed src="http://vimeo.com/moogaloop.swf?clip_id=3454392&server=vimeo.com&show_title=1&show_byline=1&show_portrait=0&color=00ADEF&fullscreen=1" type="application/x-shockwave-flash" allowfullscreen="true" allowscriptaccess="always" width="560" height="315"></embed></object><br /><a href="http://vimeo.com/3454392">The DIYbio Community - Presented at Ignite Boston 5 (2009)</a> from <a href="http://vimeo.com/macowell">mac cowell</a> on <a href="http://vimeo.com">Vimeo</a>.<br /><br /></div> <object width="560" height="340" style="float:left;"><param name="movie" value="http://www.youtube.com/v/-IIWH6Hhcnc&hl=en&fs=1&rel=0"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/-IIWH6Hhcnc&hl=en&fs=1&rel=0" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="560" height="340"></embed></object> <div style="clear:both"> </div><br /> </html>

What is DIYbio's mission?

Intro In The News Educational Equipment Projects Kits Methods DIYbio.org DIYbio googlegroup FriendFeed - DIYbio http://c.statcounter.com/5195189/0/1a5d59b0/1/0.png

DIYbio FAQ v1.5: "The biohacker's FAQ"

This FAQ for DIYbio is actively maintained by it's editors, and by you! Edit your contributions directly or email updates to the DIYbio email list, diybio@googlegroups.com.

Major contributors (in alphabetical order):

The contents of this FAQ are copyright under the OpenWetWare Copyright policy (Creative Commons Attribution-ShareAlike 3.0 Unported). When quoting any content of this FAQ elsewhere, include a full hypertext link back to the main FAQ page.

""*The* goal of DIYBIO, for me, is to reduce as much as possible the specialized equipment handicap for those who choose not to take the degree track / academic institution approach. I can become a professor of electrical engineering, or computer science, or evolutionary biology, without ever getting a degree or attending a course below the PhD level. I can't currently say the same thing about biotechnology with much confidence unless I'm lucky enough to have access to a lab. [...] DIYbio is a hardware hacking endeavor at its core, and it's the hardware hackers working hand-in-hand with the protocol authors who are laying the groundwork for making this a field open to anyone with the drive to become great at it. ""

-- Len Sassaman, DIYbio google group

Are we moving to a future where everyone performs a little genetic engineering? Is genetic engineering safe? Are GMO's safe? Is genetic engineering safe for hackers or everyone to perform? Aren't there too many risks or unknowns? Is it legal or illegal?

Today, everyone performs a "little" computer use, whereas decades ago leaders in the computer field claimed regular people would never need a computer. Decades before that, leaders in the transportation field claimed regular people would never need a car or would never need high speed travel. Eventually these technologies became usable enough for everyone, and somewhat indispensable. Looking many decades ahead, genetic engineering will likely be a common place activity, as with any technology.

Regarding whether genetic engineering is safe for hackers or for everyone, the group invites discussion. There are the key points:

There are many unknowns in genetic engineering ("We don't know").

There are many more unknowns than we currently know are unknown ("We don't know what we don't know").

There are methods to contain genetic engineering experiments to a clean laboratory with only small amounts of risk ("We can reduce the possibility of problems during experimentation").

There are unknown risks if genetic engineering experiments escape into the wild ("We don't know").

Readers are encouraged to check out "What we know--and what we don't know--about ecological risks of genetically engineered plants" as of 2001 knowledge map on risk from Robert Horn at Stanford. If you have a more recent and easy-to-read summary of Risk than the paper from 2000/2001, then add it here.

Readers are encouraged to watch the documentary, "The Future of Food" on Hulu: http://www.hulu.com/watch/67878/the-future-of-food

Open-Source Biology And Its Impact on Industry, Rob Carlson, IEEE Spectrum, 2001.

""Technology based on intentional, open-source biology is on its way, whether we like it or not. Distributed biological manufacturing is the future of the global economy and will occur as inexpensive, quality DNA sequencing and synthesis equipment becomes available to anyone. In 2050, garage biology hacking will be well under way. Fear of potential hazards should be met with increased research and education, rather than closing the door on the profound positive impacts that distributed biological technology will have on human health, human impacts on the environment, and increasing standards of living around the world. "" http://www.kurzweilai.net/articles/art0613.html?printable=1

Schmidt M, 2008. Diffusion of synthetic biology: a challenge to biosafety. Systems and Synthetic Biology. Vol.2(1-2):1-6. http://www.markusschmidt.eu/pdf/Diffusion_of_synthetic_biology.pdf

""[..] more and more people outside the traditional biotechnology community will create self-replicating machines (life) for civil and defence applications, ‘‘bio-hackers’’ will engineer new life forms at their kitchen table; and illicit substances will be produced synthetically and much cheaper. Such a scenario is a messy and dangerous one, and we need to think about appropriate safety standards now. ""

IRGC 2008. Concept note: Synthetic Biology. Risks and opportunities of an emerging field. International Risk Governance Council, Geneva. http://www.synbiosafe.eu/uploads/pdf/IRGC_ConceptNote_SyntheticBiology_Final_30April.pdf

""Suggestions have also been made for dealing with biosafety issues to do with the accidental (rather than purposeful) release of synthetic organisms. Tucker and Zilinskas (2006), for example, think that the precautionary principle should be adopted with respect to synthetic biology saying that it may be necessary to ban all uses in the open environment until a robust risk assessment can be conducted for each proposed application (p.44). Others think that this step would make research expensive and restrict synthetic biology to a few labs (Garfinkel et al. 2007). ""

What are the social, ethical, legal/patent implications of DIY Bio or home genetic engineering? How might it be best to create more social, ethical, legal/patent discussion?

These issues are discussed very well in the publications of SYNBIOSAFE, which includes discussion of DIYbio itself.

SYNBIOSAFE publications: Safety, Security and Ethical Aspects of Synthetic Biology. http://www.synbiosafe.eu/index.php?page=resources
- Schmidt et al. 2009. A priority paper for the societal and ethical aspects of synthetic biology. Systems and Synthetic Biology. Vol.3(1-4):1-2 http://www.synbiosafe.eu/uploads/pdf/Schmidt_etal-2009-SSBJ.pdf
- Kelle A. 2009. Ensuring the security of synthetic biology—towards a 5P governance strategy. Systems and Synthetic Biology. Vol.3(1-4): 85-90 http://www.synbiosafe.eu/uploads/pdf/Kelle-2009-SSBJ.pdf
- Schmidt M, Torgersen H, Ganguli-Mitra A, Kelle A, Deplazes A, Biller-Andorno N. 2008. SYNBIOSAFE e-conference: online community discussion on the societal aspects of synthetic biology. Systems and Synthetic Biology. http://www.markusschmidt.eu/pdf/SSBJ-SYNBIOSAFE_e-conference.pdfVol.2(1-2):7-17

Rob Carlson on THE ECONOMIST
- http://audiovideo.economist.com/?fr_story=706e536c2a60f103f23adcc3f0c0c76150dfbe6b&rf=bm Rob Carlson on synthetic biology. You can do a lot in your garage. A professor of biosynthesis on open-source biology, buying DNA online and the problem with patents.

Webcasts
- Patenting Synthetic Biology: A Transatlantic Perspective. http://www.synbioproject.org/events/archive/6384/ ( Go to the link and click "View Webcast".) Investments in synthetic biology research have been ramping up and the field holds significant promise across areas ranging from medicine to renewable energy. As synthetic biology moves forward, it is critical for researchers, technology developers, investors, and public policy makers to understand how the European Patent Office and the U.S. Patent and Trademark Office will react and respond to the applications covering synthetic biology inventions. This is a unique opportunity to discuss factors influencing EU and U.S. policies on the evolution of intellectual property protection for synthetic biology with experts from both sides of the Atlantic. John LeGuyader, Director TC 1600, U.S. Patent and Trademark Office; Berthold Rutz, Examiner, Directorate 2.4.01, Biotechnology, European Patent Office
- Bioethics: The Presidential Commission for the Study of Bioethical Issues, The Ritz-Carlton, Washington, D.C., July 8-9, 2010. http://www.tvworldwide.com/events/bioethics/100708/default.cfm . Drew Endy, Bonnie L. Bassler, Robert Carlson, J. Craig Venter, George Church, Kristala L. J. Prather, Allison Snow, Jim Thomas, Nancy M.P. King, Gregory Kaebnick, Allen Buchanan, David Rejeski, Markus Schmidt, Paul Root Wolpe, Amy Patterson, Michael Rodemeyer, Edward H. You.

Other Papers:

Selgelid M. 2007. The tale of two studies: Ethics, Bioterrorism, and the Censorship of Science. Hastings Center Report 37, no. 3:35-43. http://www.synbiosafe.eu/uploads///pdf/Tale%20Two%20Studies%20Final%20Printed.pdf
Rai A, and Boyle J. 2007. Synthetic Biology: Caught between Property Rights, the Public Domain, and the Commons. PLoS Biol. 13;5(3):e58 http://www.plosbiology.org/article/info:doi%2F10.1371%2Fjournal.pbio.0050058

Church G., 2005, Let us go forth and safely multiply. Nature, Vol. 438: 423. http://www.nature.com/nature/journal/v438/n7067/pdf/438423a.pdf

""A code of ethics and standards should emerge for biological engineering as it has done forother engineering disciplines. [...] Above all, outreach is required. Genetically modified products, including crops and gene-therapy drugs, have been opposed for reasons that go beyond worries about scientific uncertainties. Citizens who will gladly take recombinant-DNA drugs (such as interferon, insulinand erythropoietin) are reluctant to eat foods containing even trace amounts of recombinant DNA. Can synthetic biology gain greater public trust? We should learn from past cases; in the case of foods generated by synthetic biology, for example, we need to recognize that stakeholders include not just the farmers, but their neighbours and grocery shoppers also. [...] In addition to a code of professional ethics for synthetic biologists, we need to watch for the rare cases when they transgress. This requires not just laws, but also monitoring compliance. [..] Discussions about this have begun, including one funded by the Sloan Foundation ('Study to explore risks, benefits of synthetic genomics'). But any actions that penalize the legitimate manufacturer or user are likely to backfire, and having laws without government-mandated surveillance will be ineffective. Finally, the community needs to discuss the benefits of synthetic engineering to balance the necessary, but distracting, focus on risks. From now on, each small step towards engineering enzymatic pathways for cheaper pharmaceuticals, smart biomaterials and large-scale integrated genetic circuits should be celebrated. ""

""Monsanto Canada Inc. v. Schmeiser [2004] 1 S.C.R. 902, 2004 SCC 34 is a leading Supreme Court of Canada case on patent rights for biotechnology. The court heard the question of whether growing genetically modified plants constitutes "use" of the patented invention of genetically modified plant cells. It ruled that it does. The case drew worldwide attention."" http://en.wikipedia.org/wiki/Monsanto_Canada_Inc._v._Schmeiser

Are there Historical Precedents or Prior Cases which have Demonstrated these Issues?

U.S.

DIY/homebrew chemistry is already adversely affected by the War on Drugs via government regulations intended to limit manufacture of methamphetamine.

 There is a really interesting parallel between potential DIYbio
 regulations and attempts to quash meth production in America. Biotech-
 commentator Robert Carlson published an article in 2008 (
 http://www.springerlink.com/content/n211746672413507/ ) which confirms
 the point you mention--mom and pop (drug-manufacturing) outfits changed into cartels across
 the US border when the DOJ/DEA tried to crack down by controlling
 access to DIY-meth materials. On his blog,
 http://www.synthesis.cc/2009/08/and-the-innovation-continuesnow-for-shake-and-bake-meth.html 
 and in his new book, he predicts a similar phenomenon will befall
 biological engineering and DIY-biology if the government tries to
 restrict access to materials. Luckily for DIYbio enthusiasts, Carlson
 is also involved in some public policy and expert panels.

-- Marshall Louis Reaves, DIYbio google group

Arizona, 2009

Recent example where a homebrew chemistry project runs into danger due to an accident, perhaps causing larger scrutiny for others in the future.

A super interesting case study of this behavior(*) is homebrew bio-diesel in Arizona.
[ * - Referring to social issues and/or government regulation stemming
from bad media portrayals or accidents in homebrew experimentation. -- JC ]
Without going into too much detail, homebrewers use chemicals that can
mostly be purchased at swimming pool supply stores (lots of those in
the desert) including methanol and NaOH. Last summer, a homebrewer's
oily rags ignited methanol in his garage. An explosion and house fire
followed. A local news article about the blast:
http://www.google.com/search?q=Fumes+from+biodiesel+kit+cause+house+explosion+in+Surprise&btnI=745
You should note how demonized and terrifying making the bio-diesel
seems. The paragraphs essentially alternate between indicating safety
and overblown fears.
""We knew about (Spreadbury) doing the biodiesel but we didn't think
he was a danger to us," said neighbor Shannon Daron.
When asked if she now felt differently, Daron replied "absolutely."
The fire never spread beyond the garage and Spreadbury and his family
were not injured.
A spokesperson for the Surprise Fire Department said Sunday they're
concerned more people will turn to alternative fuels like biodiesel
with the rising price of gas.
Asst. Chief Kevin Pool worries, if not installed and maintained
properly, they could see more fires started by people making biodiesel
at home.
"You might make one little mistake like this and there could be a
tragedy," said Pool. "It's at your own risk and your neighbor's
risk."
"We just bought this house," said Daron. "We don't want it
jeopardized or our children.""
It seems like an almost nonsensical work of journalism. Homebrew
doesn't seem to get a fair play in the slightest. This was a serious
black-eye for homebrew.
Some cities in the Phoenix valley (Phoenix is a collection of
independent cities) lashed back. A "Bio-diesel Task Force" was
formed, and some jurisdictions deemed homebrewing bio-diesel as
"industrial activity" and therefore illegal in residential zones. A
very easy "fix" for overzealous authorities. I'm not sure if searches
or arrests/fines occurred. This could be a similar weapon used against
DIYbio'ers, since many materials including simple enzymes could be
"industrial" in nature. On the brighter side, some cities have adopted
a pro-homebrewer stance by taking into account safety and zoning
codes: http://tinyurl.com/ProBDcode Throughout the state, chemicals
are incredibly difficult to acquire, even in small amounts. The Meth-
trade in Arizona doesn't help either.
Although opinions differ--there are some very smart and well-
positioned advocates of homebrew bio-diesel in Arizona--with one vital
key to all of this is a separation of "safe" from "unsafe" practices.
This is something that DIYbio'ers often seem to work towards. But the
codification of best practices and vigorous dissemination of them
seems to be working in homebrewers favor when talking with regulators:
http://216.104.40.250/~biodcom1/greenbeat/soybenz/b101man/
When people ask questions of safety, can DIYbio'ers point to a "Bible"
of sorts to ask if it is a sin? This is especially important to
separate the *good* parts of DIYbio from a more dangerous fringe if
such a group exists now or in the future: We are good, they are the
bad because they violate rules X, Y, and Z. Otherwise, the whole group
gets labeled as bad, dangerous, or whatever, and this obviously leads
to being outlawed.
I know lots of people speculate about lots of futures of regulation
and public perception, but I think that lessons can be learned form
homebrew bio-diesel. A great resource is a the Desert Biofuels Blog at
http://desertbiofuels.blogspot.com

-- Marshall Louis Reaves, DIYbio google group

Who is a "biohacker"?

What do people think of when they think of a typical biohacker?
probably in the sense of the subculture (more broadly, Hacker culture)
combines the hacker ethic of biologists, programmers, DIY enthusiasts, etc.
Hacker ethic of the Homebrew Computer Club
Free software movement
people who enjoy "hacks".
- GNU is a hack: a recursive acronym for "GNU is Not Unix".
might be somewhat related to biopunk.

How can I find out more and contribute?

Many ways! Here's a brief overview:

mailing list (most activity) (note the discussion on the list's standards)
IRC (#diybio on irc.freenode.net)
local groups

So far, we mainly communicate through the mailing list. There is also a lower volume DIYbio announce mailing list, which occassionally has announcements that the community might be interested in. Also, there are groups for:

San Francisco
Chicago
New York (city)
London
Boston.
The main list is (as mentioned above) DIYBio mailing list

You're welcome to subscribe to the mailing lists- in fact, we encourage it.

There are other forums:

biopunk.org
sciencemadness.org
please expand this list

Guidelines for Posting

As the DIYBio mailing list membership grows, it is more important to follow good guidelines for easier readability within discussions. This is called Netiquette.

Please:

Follow proper quoting rules:

 One should reply using the standard technique:

    User C. wrote:
    > User B. wrote:
    > > User A. wrote:
    > > > blablabla
    > > blubberblubber
    > laberlaber 

    Your Thoughtful Reply Goes Here.

For complete information on quoting, see conventional netiquette.

When quoting another author, keep the attribution line ("On such-and-such-date, Jonathan Cline wrote:").
- Delete portions of the paragraph which do not pertain to the new reply. This is known as Trimming the post.
- Trim all quoted text to be the minimum necessary to follow the discussion.
  - Replace deleted text with "[...]" if it changes the placement of words or sentences in a paragraph.
Add your message below any quoted text. This means "write your reply at the bottom".
- Do not "top post". "Top posting" is when the reply is added above the quoted text. This is not as easy to read wen there are many replies in a thread. For this reason, do not "top post", only add the reply at the bottom. Many mail programs have a setting to "reply at top" or "reply at bottom" -- always set it to "Reply at bottom" or manually perform this action yourself. "Top posting" is considered rude by many readers.
Change the Subject when the topic changes.
Do not "bump" messages. Bumping is purposely replying and quoting an old message purely for the intention of bringing attention to the message (usually with a single line of text, consisting of "Bump!"). If there is new information, then group members will reply. If there are no replies, then wait at least two weeks before bringing up the topic again, or until there is something "new" to add to the original post.

Please see the following Internet reference for complete information:

http://www.faqs.org/rfcs/rfc1855.html

Where can I see an archive of previous DIYbio discussions and questions?

The DIYbio google group mailing list is hosted from Google Groups which allows reading prior discussions.

Some of our favorites ("member picks") include discussions on ..

Public perception
your favorite thread here

Is there a group in my area?

There's probably a group nearby- maybe at least somebody somewhat interested in getting together for lunch or maybe sitting down over a bench and doing serious experiments- at any rate, you can find out about those near you by checking out the map below or diybio.org/local.

<a href="http://maps.google.com/maps/ms?ie=UTF8&hl=en&msa=0&ll=42.358163,0.0&z=2&spn=0,0&msid=117373025318808082442.00045fd549f07830e0465&source=embed">View a larger map, or to add yourself or your group to the map.</a> You'll need to sign into your Google account in order to add a new point. Here's a <a href="http://skitch.com/jasonmorrison/bycdy/add-a-point.png-png-image-864x494-pixels-scaled-70">screenshot of how to add a new point on the map</a>. </html>

You may also be interested in other local science groups around the world:

dorkbot group ("people doing strange things with electricity")
hackerspace.
please expand this list

Are there any videos from regional groups?

DIYbio MCR

Manchester, UK group. Some photos of SwabFest plates and participants, courtesy of Hwa Young Jung. "Find out more about what we do at diybio.madlab.org.uk #diybiomcr"

http://www.flickr.com/photos/madlabuk/collections/72157626528280829/

DIYbio-NYC: Shot glass DNA extraction

DIYbio-SF: Tito's food coloring electrophoresis

DIYbio-boston: diybio visits the fablab

---

Bio-Artist Multimedia

BIO:FICTION Science, Art & Film festival 2010, Museum of Natural History, Vienna, Austria

Bio:Fiction is the world's first synthetic biology film festival. Our call for submissions in 2010 triggered 130 short film entries from 25 countries. 52 films were shortlisted and judged by an international jury composed of filmmakers and synthetic biology scientists. Several award categories will honour the best short films, covering science documentaries, science fiction films and plain fictional narratives.

http://bio-fiction.com/videos

What does a Garage Lab look like?

http://farm3.static.flickr.com/2478/4044058001_71291f7196_m.jpg

Post pictures of your own home laboratory setup, and view some: http://www.flickr.com/groups/diylabs/

-- From Raymond McCauley, DIYbio google group

Has DIYbio been in the news?

Yes. Frequently! See In The News for a significant list of articles.

What are some educational resources for DIYBio and Biology? What are all these terms and technologies DIYBio keeps discussing?

See DIYBio FAQ: Education & Resources

How can I grow and engineer yeast? How can I grow and engineer bacteria?

See DIYBio FAQ: Projects

What equipment do I need to perform DIYBio-related projects?

See DIYBio FAQ: Equipment

What is open source hardware?

http://p2pfoundation.net/Open_source_hardware

"Open Source Hardware is hardware that keeps its designs available in a way similar to the open source in software." There is no defacto license for open source hardware yet. Some websites (like ponoko, thingiverse, unptnt) put hardware CAD files under a "Creative Commons" license. However, it's still unknown how this is likely to interface with the legal systems around the world (i.e., patents). And it's not necessarily true that putting something directly into the public domain is the best way to go either. So, the future is presently unclear- in terms of legal issues.

DIYbio has many big supporters of standardized packaging formats (like .tar.gz, .deb, .tar, .rpm, etc.) for automatic downloading of hardware components and instructions on how to build the components. There are some sites that almost implement this (but not quite) such as instructables, ponoko, thingiverse, odesigns, unptnt, etc.

Open Source Everything project, adciv.org
Open Source Ecology (Factor E Farm, openfarmtech, etc.)
ToolBook (just some links to other articles about ToolBook) (infrastructure)

'Slashdot discussions

Open Source Ecology

Open source hardware includes large systems.

 This is Chris Fornof with Open Source Ecology, http://opensourceecology.org 
 We're attempting to create a Global Village Construction Set 
 (GVCS, with the aim of creating a "civilization starter kit". 
 See the TED talk, http://www.ted.com/talks/marcin_jakubowski.html

Projects

What Projects has DIYBio completed? What projects are DIYBio contributors working on now? Who is working on what? Who do I contact to offer to collaborate on a project?

See DIYBio FAQ: Projects.

Please add your own project info to the DIYBio FAQ: Projects topic!

Appendix 1 - list of Synthetic Biology Companies

Appendix 2 - List of Equipment Suppliers

See DIYBio FAQ: Equipment for new/used/refurbished equipment suppliers.

Appendix 3 - Laboratory Basics

See DIYBio FAQ: Methods for basic lab technique, including sterilization, using animals, etc.

Intro In The News Educational Equipment Projects Kits Methods DIYbio.org DIYbio googlegroup FriendFeed - DIYbio http://c.statcounter.com/5195189/0/1a5d59b0/1/0.png

DIYbio FAQ v1.5: "The biohacker's FAQ"

This FAQ for DIYbio is actively maintained by it's editors, and by you! Edit your contributions directly or email updates to the DIYbio email list, diybio@googlegroups.com.

Major contributors (in alphabetical order):

The contents of this FAQ are copyright under the OpenWetWare Copyright policy (Creative Commons Attribution-ShareAlike 3.0 Unported). When quoting any content of this FAQ elsewhere, include a full hypertext link back to the main FAQ page.

@@ Line 12: / Line 12: @@
 * 1.3 - expand projects sections.  Add Laboratory Basics section. --'''[[User:Jonathan Cline|jcline@ieee.org]]'''
 * 1.4 - add 'Methods' section, move Laboratory Basics into 'Methods' --'''[[User:Jonathan Cline|jcline@ieee.org]]'''
+* 1.5 - Add 'News' section, move news articles there. [[User:Jonathan Cline|jcline@ieee.org]] 23:40, 23 May 2011 (EDT)
+* 1.6 - Multiple updates to project section [[User:Jonathan Cline|jcline@ieee.org]] 00:20, 6 September 2012 (EDT)
 = What is DIYbio, as an organization? =
@@ Line 31: / Line 33: @@
 == What is DIYbio's mission? ==
+{{DIYbio:Top}}
@@ Line 270: / Line 274: @@
 == Are there any videos from regional groups? ==
+=== DIYbio MCR ===
+Manchester, UK group.  Some photos of SwabFest plates and participants, courtesy of Hwa Young Jung.  "Find out more about what we do at diybio.madlab.org.uk #diybiomcr"
+: http://www.flickr.com/photos/madlabuk/collections/72157626528280829/
 === DIYbio-NYC: Shot glass DNA extraction ===
 <youtube align="left">s2HPVs25HlY</youtube> <html><div style="clear:both;"></div></html>
@@ Line 276: / Line 284: @@
 === DIYbio-boston: diybio visits the fablab ===
 <youtube align="left">oQ3BRKAsG0I</youtube> <html><div style="clear:both;"></div></html>
+---
+=== Bio-Artist Multimedia ===
+: '''BIO:FICTION Science, Art & Film festival 2010''', Museum of Natural History, Vienna, Austria
+: Bio:Fiction is the world's first synthetic biology film festival. Our call for submissions in 2010 triggered 130 short film entries from 25 countries.  52 films were shortlisted and judged by an international jury composed of filmmakers and synthetic biology scientists. Several award categories will honour the best short films, covering science documentaries, science fiction films and plain fictional narratives.
+:: http://bio-fiction.com/videos
 == What does a Garage Lab look like? ==
@@ Line 286: / Line 303: @@
 == Has DIYbio been in the news? ==
-Yes.
+Yes.  Frequently!  '''See [[DIYbio/FAQ/News|In The News]] for a significant list of articles.'''
-* 2011-01-03: [http://bbedit.sx.atl.publicus.com/apps/pbcs.dll/article?AID=/20110103/NEWS0107/101030313/1159 Citizen scientists carving out new roles via the Web] (bendbulletin.com)
-* 2011-01-02: [http://exi-chat.blogspot.com/2011/01/exi-transcending-human-diy-style.html Re: Transcending the Human, DIY Style] (exi-chat.blogspot.com)
-* 2011-01-02: [http://groups.google.com/group/diybio/browse_thread/thread/f6e0cdc34695ddba London Hackspace - Biohacking projects to start Saturday January 15 2011]
-* 2011-01-01: [http://scienceandtechnologiez.blogspot.com/2011/01/event-in-singapore.html Event in Singapore (January 8 2011)] (scienceandtechnologiez.blogspot.com) [http://groups.google.com/group/diybio/browse_thread/thread/d879a6b89c2f8551]
-* 2010-12-31: [http://www.genomeweb.com/blog/use-data-all-theyre-worth Use the Data for All They're Worth] (genomeweb.com) (re: Journal of Participatory Medicine)
-* 2010-12-31: [http://letters.cunningprojects.com/?p=142 DIYbio, a 2010 year in review] (letters.cunningprojects.com)
-* 2010-12-30: [http://www.wired.com/threatlevel/2010/12/transcending-the-human-diy-style/ Transcending the Human, DIY Style] (Wired)
-* 2010-12-27: [http://www.nytimes.com/2010/12/28/science/28citizen.html Managing Scientific Inquiry in a Laboratory the Size of the Web] (NYTimes)
-* 2010-12-24: [http://www.sciencemag.org/content/330/6012/1766.full#sec-3 Science, Law Enforcement Build Biotech Bridges] (Science Magazine/AAAS)
-* 2010-12-23: [http://www.jopm.org/evidence/research/2010/12/23/citizen-science-genomics-as-a-model-for-crowdsourced-preventive-medicine-research/ Citizen Science Genomics as a Model for Crowdsourced Preventive Medicine Research] (Journal of Participatory Medicine)
-* 2010-12-22: [http://www.nature.com/nature/journal/v468/n7327_supp/full/468S21a.html Technology: A flavour of the future] (nature.com)
-* 2010-12-22: [http://www.good.is/post/future-foragers-dunne-raby-redesign-human-digestion-to-redefine-food/ Future Foragers: Dunne & Raby Redesign Human Digestion to Redefine "Food"] (good.is)
-* 2010-12-22: [http://motherboard.tv/2010/12/22/biotech-for-the-masses Biotech for the masses] (motherboard.tv)
-* 2010-12-21: [http://bio.tmplab.org/2010/12/prochain-meeting-biohacklab-le-mardi-11-janvier/ Biohacklab: Prochain meeting: Mardi 11 Janvier & atelier Neurohack] (bio.tmplab.org)
-* 2010-12-21: [http://thinktech.honadvblogs.com/2010/12/21/identifying-the-sea-changes-and-opportunities-in-biohacking/ Identifying the sea changes and opportunities in "biohacking"] (thinktech.honadvblogs.com)
-* 2010-12-21: [http://openpcr.org/2010/12/openpcr-at-the-grenswerk-art-festival-enschede-the-netherlands/ OpenPCR at the Grenswerk Art Festival (Enschede, the Netherlands)] (openpcr.org)
-* 2010-12-19: [http://www.nytimes.com/2010/12/19/fashion/19amateur.html Turning Geek Into Chic] (NY Times)
-* 2010-12-17: [http://www.genspace.org/blog/?p=32 Genspace: Open for Business] (genspace.org) ([http://bookhling.wordpress.com/2010/12/17/genspace-grand-opening/], [http://cosmiclog.msnbc.msn.com/_news/2010/12/17/5670056-biotech-hacker-lab-opens-in-nyc msnbc])
-* 2010-12-17: [http://www.observer.com/2010/media/despite-concerns-brooklyns-new-lab-not-making-meth Despite Concerns, Brooklyn's New Lab Is Not For Making Meth] (observer.com)
-* 2010-12-16: [http://www.wired.com/wiredscience/2010/12/genspace-diy-science-laboratory/ DIY Biotech Hacker Space Opens in NYC] (Wired) ([http://www.genspace.org/blog/?p=68])
-* 2010-12-16: [http://www.nature.com/news/2010/101216/full/news.2010.680.html Synthetic biology needs oversight not over-regulation, commission finds.] (Nature News) also on [http://www.scientificamerican.com/article.cfm?id=us-report-sets-ground-rules SciAm], [http://www.kurzweilai.net/u-s-bioethics-commission-gives-green-light-to-synthetic-biology KurzweilAI], [http://www.nytimes.com/2010/12/16/science/16synthetic.html NY Times], [http://blogs.wsj.com/health/2010/12/16/presidential-advisory-panel-takes-middle-course-on-synthetic-biology/ Wall Street Journal], [http://www.latimes.com/health/la-sci-synthetic-biology-20101216,0,5846571.story LA Times], [http://www.the-scientist.com/news/display/57816/ The Scientist]
-* 2010-12-16: [http://www.nytimes.com/2010/12/16/technology/personaltech/16basics.html Home Labs on the Rise for the Fun of Science] (NY Times)
-* 2010-12-16: [http://mariakonovalenko.wordpress.com/2010/12/16/melanie-swan-a-summary-of-important-themes-in-aging-research/ Bay Area Aging: A summary of important themes in aging research (Melanie Swan)] (mariakonovalenko.wordpress.com)
-* 2010-12-15: [http://www.nature.com/news/2010/101215/full/468880a.html The rise of the genome bloggers: Hobbyists add depth to ancestry trawls.] (Nature News)
-* 2010-12-07: [http://www.ethicalcorp.com/content.asp?ContentID=7215 Synthetic biology: The innovation and bioterrorism balance] (ethicalcorp.com)
-* 2010-12-07: [http://diybio.org/2010/12/07/piracy-in-the-age-of-diybio Piracy in the age of DIYbio] (diybio.org)
-* 2010-12-05: [http://scenariosandstrategy.wordpress.com/2010/12/05/wetware/ Wetware] (scenariosandstrategy.wordpress.com)
-* 2010-12-02: [http://marcuswohlsen.com/2010/12/02/biopunk-diy-scientists-hack-the-software-of-life-coming-spring-2011/ Biopunk: DIY Scientists Hack the Software of Life, coming spring 2011] (marcuswohlsen.com)
-* 2010-12-01: [http://howplantswork.wordpress.com/2010/12/01/diy-plant-genetic-engineering/ DIY Plant Genetic Engineering] (howplantswork.wordpress.com)
-* 2010-11-29: [http://www.arsbiologica.org/2010/11/biocurious-interview-with-joseph.html BioCurious? Interview with Joseph Jackson about DIY Biotech] (arsbiologica.org)
-* 2010-11-26: [http://noodlemaz.wordpress.com/2010/11/26/the-future-of-science/ The Future of Science?] (noodlemaz.wordpress.com)
-* 2010-11-25: [http://bioethics2010bu.blogspot.com/2010/11/us-artificial-life-to-take-middle.html US 'artificial life' to take middle ground] (bioethics2010bu.blogspot.com) or The Scientist
-* 2010-11-22: [http://diybio.org/2010/11/22/i-brew-therefore-i-am I Brew, Therefore I am] (diybio.org)
-* 2010-11-19: [http://www.synthesis.cc/2010/11/more-stem-cell-magic.html More Stem Cell Magic] (synthesis.cc)
-* 2010-11-14: [http://biologigaragen.org/?p=55 DIYbio at the iGEM conference] (biologigaragen.org)
-* 2010-11-12: [http://blog.p2pfoundation.net/critical-art-ensemble-garage-biology-today/2010/11/12 Critical Art Ensemble on the import of garage biology today] (p2pfoundation.net)
-* 2010-11-05: [http://diybio.org/2010/11/05/diybio-and-the-man DIYbio and the "MAN"] (diybio.org)
-* 2010-11-04: [http://diybio.org/2010/11/04/whither-biohackers/ Whither “Biohackers?”] (diybio.org)
-* 2010-10-30: [http://ing.dk/artikel/113418-open-source-biologi-baner-vej-for-bioterror-fra-garagen Open source-biologi baner vej for bioterror fra garagen] (ing.dk)
-* 2010-10-23: [http://chaaraka.blogspot.com/2010/10/diybio-gets-little-more-localbangalore.html DIYBio gets a little more local...Bangalore just got one - is there one in your area?] (chaaraka.blogspot.com)
-* 2010-10-19: [http://blogs.ischool.utexas.edu/digitalcuration/2010/10/19/bio-hackers-not-just-a-title-for-a-bad-science-fiction-movie/ Bio-Hackers – Not Just a Title for a Bad Science Fiction Movie] (blogs.ischool.utexas.edu)
-* 2010-10-18: [http://futurismic.com/2010/10/18/garage-ribofunk-going-mainstream/ Garage ribofunk going mainstream] (futurismic.com)
-* 2010-10-15: [http://www.technomage.org/2010/10/diy-biohacking.html DIY BioHacking] (technomage.org)
-* 2010-10-15: [http://science.slashdot.org/story/10/10/15/1452226/The-Spread-of-Do-It-Yourself-Biotech The Spread of Do-It-Yourself Biotech] (slashdot.org)
-* 2010-10-13: [http://wetware-and-hard-science.wetmachine.com/content/diy-biotechnology/comment-page-1 DIY biotechnology] (wetmachine.com)
-* 2010-10-12: [http://blogs.kentlaw.edu/islat/2010/10/biohack-the-planet-a-new-generation-of-hackers-sweep-across-the-country.html Biohack the Planet! A New Generation of Hackers Sweep Across the Country] (blogs.kentlaw.edu)
-* 2010-10-11: [http://www.genomicslawreport.com/index.php/2010/10/11/a-do-it-yourself-genomic-challenge-to-myriad-the-fda-and-the-future-of-genetic-tests A Do-It-Yourself Genomic Challenge to Myriad, the FDA and the Future of Genetic Tests] (genomicslawreport.com)
-* 2010-10-11: [http://blog.makezine.com/archive/2010/10/garage_biotech_in_nature_magazine.html Garage biotech in Nature magazine] (makezine.com) ([http://journal.ocular-witness.com/?p=4362 crosspost]; [http://lavaamp.wordpress.com/2010/10/12/did-anyone-else-see-lavaamp-mentioned-in-nature-last-week/ Lava-Amp])
-* 2010-10-10: [http://futurememes.blogspot.com/2010/10/consumer-genomic-testing-update.html Consumer genomic testing update] (futurememes.blogspot.com)
-* 2010-10-08: [http://orthonormalruss.blogspot.com/2010/10/on-importance-of-sequenced-model.html On the importance of sequenced model organisms, & a crude taxonomy of their users] (orthonormalruss.blogspot.com)
-* 2010-10-08: [http://www.genomeweb.com/blog/garage-lab The Garage Lab] (genomeweb.com)
-* 2010-10-07: [http://pipeline.corante.com/archives/2010/10/07/more_on_garage_biotech.php More on Garage Biotech] (pipeline.corante.com)
-* 2010-10-06: [http://www.nature.com/nature/journal/v467/n7316/full/467634a.html Editorial: Garage biology: Amateur scientists who experiment at home should be welcomed by the professionals] (nature.com)
-* 2010-10-06: [http://www.nature.com/news/2010/101006/full/467650a.html Garage biotech: Life hackers] (nature.com)
-* 2010-10-04: [http://ellingtonlab.org/blog/?p=153 On spoofing] (ellingtonlab.org)
-* 2010-10-01: [http://blog.makezine.com/archive/2010/10/diybio_nyc_on_the_biobus_1.html DIYbio NYC on the BioBus] (makezine.com)
-* 2010-09-29: [http://sugru.com/blog/citizen_science/ Citizen Science and Biocurious] (sugru.com)
-* 2010-09-28: [http://blog.makezine.com/archive/2010/09/interview_with_melanie_swan_of_diyg.html Interview with Melanie Swan of DIYgenomics] (makezine.com)
-* 2010-09-27: [http://diybionyc.blogspot.com/2010/09/diybio-nycbiobus-collaboration-wins.html DIYbio NYC/BioBus Collaboration Wins MAKE Magazine Editors' Choice Award] (diybionyc.blogspot.com)
-* 2010-09-27: [http://www.livescience.com/health/future-synthetic-biology-research-garage-biology-100927.html Genetic Science Oozes Out of Amateurs' Garages] (livescience.com)
-* 2010-09-25: [http://ellingtonlab.org/blog/?p=104 On curiosity] (ellingtonlab.org)
-* 2010-09-23: [http://www.mercurynews.com/ci_16158406 Biohackers aim to open Silicon Valley lab for group research and lessons] (mercurynews.com)
-* 2010-09-20: [http://www.medgadget.com/archives/2010/09/30000_given_by_200_people_to_open_biotechnology_hackerspace.html $30,000 Given by 200+ People to Open Biotechnology Hackerspace] (medgadget.com)
-* 2010-09-18: [http://openwear.org/blog/?p=498 An interview with Mark Frauenfelder] (openwear.org)
-* 2010-09-17: [http://blog.kickstarter.com/post/1138357266/biocurious-and-the-diy-science-movement BioCurious and the DIY Science Movement] (kickstarter.com)
-* 2010-09-15: [http://sciencepark.cc/2010/09/15/quick-thoughts-on-the-bioethics-commission-meeting/ Quick thoughts on the bioethics commission meeting] (sciencepark.cc)
-* 2010-09-11: [http://hatewasabi.wordpress.com/2010/09/11/the-futur-of-biotech-patents-part-1/ The future of biotech patents] (hatewasabi.wordpress.com)
-* 2010-09-01: [http://www.nature.com/nm/journal/v16/n9/full/nm0910-953.html Personalized investigation] (nature.com)
-* 2010-09-01: [http://www.hplusmagazine.com/editors-blog/biotech-revolution-must-start-education Biotech revolution must start with education] (hplusmagazine.com)
-* 2010-09-01: [http://www.genengnews.com/gen-articles/do-it-yourself-bioengineers-bedeviled-by-society-s-paranoia/3383/ Do-It-Yourself Bioengineers Bedeviled by Society's Paranoia] (genengnews.com)
-* 2010-08-25: [http://www.boingboing.net/2010/08/25/itty-bitty-hydroponi.html itty bitty hydroponic grow box] (boingboing.net)
-* 2010-08-24: [http://www.biotechniques.com/news/biotechniquesNews/biotechniques-301745.html Cheap PCR: new low cost machines challenge traditional designs] (biotechniques.com)
-* 2010-08-24: [http://euroscientist.com/2010/08/157/ Biohackers – the geneticists in the garage] (euroscientist.com)
-* 2010-08-18: [http://boingboing.net/2010/08/18/otyp-nears-kickstart.html Otyp nears Kickstarter goal to make DNA thermal cyclers for high schoolers] (boingboing.net)
-* 2010-08-17: [http://2020science.org/2010/08/17/synthetic-biology-ethics-and-the-hacker-culture/ Synthetic biology, ethics, and the hacker culture] (2020science.org)
-* 2010-08-16: [http://www.huffingtonpost.com/jason-silva/otyp-is-making-biotech-co_b_684002.html OTYP is Making Biotech Cool] (huffingtonpost.com)
-* 2010-08-13: [http://www.homelandsecuritynewswire.com/garage-lab-bugs-spread-bioscience-increases-bioterrorism-risks Garage-lab bugs: spread of bioscience increases bioterrorism risks] (homelandsecuritynewswire.com)
-* 2010-08-03: [http://singularityhub.com/2010/08/03/making-the-modern-do-it-yourself-biology-laboratory-video/ Making the modern do-it-yourself biology laboratory (video)] (singularityhub.com)
-* 2010-08-03: [http://reason.com/archives/2010/08/03/big-tent-open-science-summit-c Citizen Science, Microfinanced Research, Patent Trolls, and Pharma Prizes: A Final Dispatch from Open Science Summit] (reason.com)
-* 2010-08-02: [http://www.boston.com/business/healthcare/articles/2010/08/02/biotech_movement_hopes_to_spur_rise_of_citizen_scientists/# Biotech movement hopes to spur rise of citizen scientists] (boston.com)
-* 2010-07-30: [http://singularityhub.com/2010/07/30/open-science-summit-2010-%E2%80%93-thursday-review/# Review of Open Science Summit 2010 (Thursday session)] (singularityhub.com)
-* 2010-07-30: [http://blogs.nature.com/news/thegreatbeyond/2010/07/crowdsourcing_science_funding_1.html Crowd-sourced science funding] (blogs.nature.com)
-* 2010-07-30: [http://reason.com/archives/2010/07/30/scenes-from-the-open-science-s Scenes from the Open Science Summit] (reason.com)
-* 2010-07-15: [http://www.wired.co.uk/news/archive/2010-07/15/eri-gentry-garage-biotech-revolution Eri Gentry's biotech revolution] (wired.co.uk) also on [http://diybio.org/2010/08/03/biocurious-eri-gentrys-garage-biotech-revolution/ diybio.org]
-* 2010-07-14: [http://www.theatlantic.com/science/archive/2010/07/diy-biotechnologists-go-looking-for-a-bigger-garage/59701/ DIY Biotechnologists Go Looking for a Bigger Garage] (theatlantic.com)
-* 2010-07-07: [http://www.xconomy.com/san-francisco/2010/07/07/the-open-science-shift/ The Open Science Shift] (xconomy.com)
-* 2010-07-07: [http://www.fieldtest.us/bio/blog/?p=15 A new biology in the twenty first century: the project] (fieldtest.us)
-* 2010-07-06: [http://www.iftf.org/node/3526?utm_source=twitterfeed&utm_medium=twitter Curing Cancer in a Garage?] (iftf.org)
-* 2010-07-05: [http://www.boingboing.net/2010/07/05/help-fund-a-hackersp.html Help fund a hackerspace for biology] (boingboing.net)
-* 2010-07-05: [http://letters.cunningprojects.com/?p=125 Biocurious; a Hackerspace for Biotechnology. Please Help!]
-* 2010-06-30: [http://www.technewsworld.com/rsstory/70314.html?wlc=1277928778 The New Hacker Hobby That Will Change the World] (technewsworld.com); ([http://news.ycombinator.com/item?id=1483328 Hacker News])
-* 2010-06-29: [http://www.prnewswire.com/news-releases/responsible-science-for-do-it-yourself-biologists-97362669.html Responsible science for DIY biologists] (prnewswire.com)
-* 2010-06-26: [http://titojankowski.com/?p=335 Let's get the biotech revolution started - support biocurious!]
-* 2010-06-22: [http://doctoralicebell.blogspot.com/2010/06/storm-royal-society.html Storm the Royal Society?]
-* 2010-06-22: [http://www.juneauempire.com/stories/062210/loc_657804475.shtml The science behind the tour]
-* 2010-06-22: [http://www.annarbor.com/entertainment/parenting/citizen-scientists-environmental-monitoring-for-kids-and-adults/ Citizen scientists: easy ideas for kids and adults to study the environment] (annarbor.com)
-* 2010-06-22: [http://www.pr-inside.com/institute-for-the-future-announces-bodyshock-r1962896.htm Institute for the Future Announces BodyShock: Call for Entries] (pr-inside.com)
-* 2010-06-22: [http://mobihealthnews.com/8044/five-mobile-health-contests-you-should-know/ Five mobile health contests you should know] (mobihealthnews.com)
-* 2010-06-21: [http://www.molecularist.com/lifeblog/2010/06/do-we-need-a-diybio-academy.html Do we need a DIYbio Academy?] (molecularist.com)
-* 2010-06-21: [http://blog.ponoko.com/2010/06/21/biotech-tools/ Biotech Tools] (ponoko.com)
-* 2010-06-16: [http://blogs.discovermagazine.com/sciencenotfiction/2010/06/16/bringing-biohacking-to-the-masses/ Bringing biohacking to the masses] (blogs.discovermagazine.com)
-* 2010-06-16: [http://www.sciencedaily.com/releases/2010/06/100616102900.htm Citizen Science: Birders Contribute Valuable Data on Invasive Plant Species] (sciencedaily.com)
-* 2010-06-16: [http://www.majorspoilers.com/archives/41758.htm/ Fluorescent Black Arrives in July]
-* 2010-06-14: [http://www.iftf.org/node/3512 Recognizing Lightweight Innovation: Key Characteristics and Technology Drivers] (iftf.org)
-* 2010-06-11: [http://hackaday.com/2010/06/11/cockroach-pimps-a-sweet-ride/ Cockroach pimps a sweet ride] (hackaday.com)
-* 2010-06-07: [http://www.fightaging.org/archives/2010/06/the-tumbling-walls-of-formal-science.php The Tumbling Walls of Formal Science] (fightaging.org)
-* 2010-06-03: [http://openpcr.org/2010/06/the-importance-of-speed-in-pcr/ The importance of speed in PCR] (openpcr.org)
-* 2010-06-01: [http://www.poptranshumanism.com/2010/06/not-so-scary-synthetic-life/ Not so scary: synthetic life] (poptranshumanism.com)
-* 2010-06-01: [http://www.nationaldefensemagazine.org/archive/2010/June/Pages/GrowingPublicInterestInGeneticScience.aspx Growing Public Interest In Genetic Science Sparks Some Bio-Security Concerns] (National Defense Magazine)
-* 2010-05-25: [http://reason.com/archives/2010/05/25/whos-afraid-of-synthetic-biolo Who's afraid of synthetic biology?] (reason.com)
-* 2010-05-19: [http://hplusmagazine.com/articles/science/citizen-scientist-joseph-jackson-and-new-open-source Citizen-Scientist Joseph Jackson and the New Open Source] (hplusmagazine.com)
-* 2010-05-20: [http://radar.oreilly.com/2010/05/make-offs-diy-indie-innovation.html Make-offs: DIY indie innovations. How low-cost, open-source tools are energizing DIY.] (O'Reilly Radar)
-* 2010-05-04: [http://burnafterreading.nationaljournal.com/2010/05/citizen-scientists-attract-fbi.php Citizen Scientists Attract FBI's WMD Unit] (Burn After Reading)
-* 2010-04-26: [http://www.boston.com/news/science/articles/2010/04/26/project_helps_amateurs_explore_their_genomes_via_dna_cocktail/ Amateurs explore their genomes via DNA cocktail] (boston.com)
-* 2010-04-23: [http://ing.dk/artikel/108211 Gør-det-selv-biotek er på vej til din garage] (Ingeniøren)
-* 2010-04-09: [http://subrealism.blogspot.com/2010/04/garage-biology.html Garage biology]
-* 2010-04-09: [http://fabbaloo.com/blog/2010/4/9/3d-printing-aids-biohacking.html 3D printing aids biohacking] (fabbaloo.com)
-* 2010-04-09: [http://biotechview.blogspot.com/2010/04/hacking-dna.html Bioengineering technology is maturing, and so is its vocabulary]
-* 2010-03-31: [http://news.bbc.co.uk/2/hi/technology/8595734.stm Life hacking with 3D printing and DIY DNA kits] (BBC)
-* 2010-03-28: [http://evolvingtrends.wordpress.com/2010/03/28/the-shift-from-top-down-to-bottom-up-production/ The shift from top-down to bottom-up production] (brief mention)
-* 2010-03-25: [http://diybio4beginners.blogspot.com/2010/03/andrew-hessel-talks-about-synthetic.html Andrew Hessel talks about synthetic biology and diybio] (diybio4beginners)
-* 2010-03-24: [http://www.genomeweb.com/blog/garage-biology-bad-science Garage Biology Bad for Science?]
-* 2010-03-23: [http://scienceblogs.com/oscillator/2010/03/diybio_and_the_gentleman_scien.php DIYbio and the Gentleman Scientist]
-* 2010-03-12: [http://pipeline.corante.com/archives/2010/03/12/garage_biotech.php Garage biotech] (In The Pipeline)
-* 2010-03-11: [http://due-diligence.typepad.com/blog/2010/03/the-roving-eye-clamatology-a-biogarage-in-silicon-valley-mickey-the-crony-capitalist.html The Roving Eye: Clamatology, A Bio-Garage In Silicon Valley, Mickey The Crony Capitalist]
-* 2010-03-11: [http://www.nanotech-now.com/news.cgi?story_id=37182 BioSecurity: How synthetic biology is changing the way we look at biology and biological threats]
-* 2010-03-08: [http://www.synthesis.cc/2010/03/garage-biology-in-silicon-valley.html Garage Biology in Silicon Valley]; see it on [http://blog.makezine.com/archive/2010/03/garage_biohacking_in_silicon_valley.html Make Magazine], [http://www.kk.org/thetechnium/archives/2010/03/dyi_garage_biot.php The Technium (Kevin Kelly)], ...
-* 2010-03-07: [http://www.moneyweb.com/mw/view/mw/en/page292681?oid=308569&sn=2009+Detail+no+image&pid=292681 The promise of biotech]
-* 2010-03-04: [http://www.nytimes.com/2010/03/07/magazine/07letters-t-002.html Letters: Do it yourself genetic engineering] (NY Times)
-* 2010-03-03: [http://www.citypaper.com/digest.asp?id=19882 Biology Student to Hold Biohacking Meeting at CCBC]
-* 2010-03-02: [http://www.boingboing.net/2010/03/02/inexpensive-gene-cop.html Inexpensive gene copier for DIY molecular biology] (boingboing.net)
-* 2010-02-26: [http://www.scienceline.org/2010/02/26/biotech-on-a-budget/ Biotech on a Budget]
-* 2010-02-16: [http://blogs.kentlaw.edu/islat/2010/02/diy-geneticsbiotechnology-by-parents-artists-andpotential-terrorists.html DIY Genetics-Biotechnology by Parents, Artists, and...Potential Terrorists] (blogs.kentlaw.edu)
-* 2010-02-16: [http://hplusmagazine.com/articles/toys-tools/hackerspace-your-garage-downloading-diy-hardware-over-web From Hackerspace To Your Garage: Downloading DIY Hardware Over the Web] (H+ Magazine)
-* 2010-02-14: [http://www.popsci.com/technology/article/2010-02/future-biology-lies-designer-organisms The wild world of DIY synthetic biology: Get your designer life forms here!] (popsci.com)
-* 2010-02-14: [http://www.nytimes.com/2010/02/14/magazine/14Biology-t.html?partner=rss&emc=rss Do-it-yourself genetic engineering] (NY Times)
-* 2010-02-12: [http://diybio.org/2010/02/12/dna-discovery-in-middle-school DNA Discovery in Middle School] (diybio.org)
-* 2010-02-02: [http://chaosradio.ccc.de/cre143.html Biohacking - Auf der Suche nach Hacks und Exploits in Molekülen und Gensträngen] (Chaos Radio Podcast Network)
-* 2010-01-25: [http://hplusmagazine.com/articles/bio/why-diy-bio Why DIY Bio?] (H+ Magazine)
-* 2010-01-25: [http://www.popsci.com/technology/article/2010-01/open-source-lab-promises-free-dna-parts-bioengineers Open-Source Lab Promises Free DNA Parts for Bioengineers] (popsci.com)
-* 2010-01-22: [http://hplusmagazine.com/articles/bio/diy-bio-growing-movement-takes-aging DIYbio: Growing movement takes on aging] in [http://hplusmagazine.com/ H+ Magazine]; discussed on [http://science.slashdot.org/story/10/01/26/165254/Open-Source-Software-Meets-Do-It-Yourself-Biology Slashdot], [http://www.reddit.com/r/Cryptogon/comments/aufme/diy_bio_a_growing_movement_takes_on_aging_h/ reddit] and [http://news.ycombinator.com/item?id=1081033 ycombinator hackernews]; [http://futurismic.com/2010/01/27/garage-ribofunk-redux-diy-biohacking-gaining-popularity/ futurismic];
-* 2010-01-15: [http://has100ideas.com/blog/culturing-bioluminescent-microbes-part-1 Culturing bioluminescent microbes, part 1] (has100ideas.com)
-* 2010-01-10: [http://www.wfs.org/Dec09-Jan10/V_Hessel.htm Reinventing the Pharmaceutical Industry, without the Industry] (The Futurist)
-* 2009-12-27: [http://www.npr.org/templates/story/story.php?storyId=121954328&ft=1&f=1007 Taking Biological Research Out Of The Laboratory] (NPR)
-* 2009-12-20: [http://www.sfgate.com/cgi-bin/article.cgi?f=/c/a/2009/12/20/MNFT1B1899.DTL Do-it-yourself biology grows with technology] (SF Chronicle)
-* 2009-12-14: [http://www.molecularist.com/lifeblog/2009/12/the-need-for-plain-english-diybio-safety-guidelines.html The need for plain English diybio safety guidelines]
-* 2009-12-14: [http://www.markusschmidt.eu/pdf/09_12_profil_bio_bastler.pdf Bio-Bastler. „Kreative Wissenschaftsbürger“.] (Profil)
-* 2009-11-20: [http://www.welt.de/wissenschaft/article5275006/Gen-Manipulation-am-heimischen-Kuechentisch.html?print=yes#reqdrucken Gen-Manipulation am heimischen Küchentisch] (welt.de)
-* 2009-11-13: [http://www.nycresistor.com/2009/11/13/diybio-at-nyc-resistor/ diybio-nyc at nycresistor]
-* 2009-10-31: [http://pimm.wordpress.com/2009/10/31/lavaamp-cheapest-pocket-pcr-thermocycler-dreamed-for-diy-biologists/ LavaAmp: Cheap Pocket PCR Thermocycler Dreamed for DIY Biologists]
-* 2009-09-11: [http://www.wired.co.uk/wired-magazine/archive/2009/09/start/synthetic-biology-will-bring-us-a-slimy,-moist-future?page=all Synthetic biology will bring us a slimy, moist future] (wired.co.uk)
-* 2009-09-03: [http://www.economist.com/printedition/displaystory.cfm?story_id=14299634 Tinkering with DNA] (The Economist) paywall alert
-* 2009-08-26: [http://www.synthesis.cc/2009/08/and-the-innovation-continuesnow-for-shake-and-bake-meth.html And the Innovation Continues...Starting with Shake and Bake Meth!] (synthesis.cc)
-* 2009-08-19: [http://mikecaulfield.com/2009/08/19/diybio-and-authentic-learning/ DIYbio and Authentic Learning]
-* 2009-08-06: [http://biodisplay.tyrell.hu/2009/08/06/diy-bio-groups-forming/ DIY bio groups forming]
-* 2009-08-01: [http://www.the-scientist.com/2009/08/1/17/1/ Am I a biohazard?] (The Scientist)
-* 2009-07-25: [http://bogdan.org.ua/2009/07/25/diybio-biohackers-open-source-medicine-openwetware.html DIYbio, biohackers, and Open Source Medicine]
-* 2009-07-20: [http://mycorant.com/diy-bio-considers-mushroom-identification/ DIYbio considers mushroom identification] (mycorant.com)
-* 2009-06-18: [http://invivia.wordpress.com/2009/07/19/cnc-plotter-a-platform-for-diy-biorapid-prototypingsculpture-image-experiments/ CNC Plotter: A platform for DIY Bio/rapid-prototyping/sculpture-image experiments] (invivia.com)
-* 2009-06-15: [http://hplusmagazine.com/articles/bio/darning-genes-biology-homebody Darning Genes: Biology for the Homebody]
-* 2009-06-12: [http://science.slashdot.org/article.pl?sid=09/06/12/0352217 Teen Diagnoses Her Own Disease In Science Class] (slashdot.org); [http://www.cnn.com/2009/HEALTH/06/11/teen.self.diagnosis/index.html cnn]
-* 2009-06-03: [http://www.synthesis.cc/2009/06/another-step-toward-diystemcells.html Another Step Toward DIYStemCells] (synthesis.cc)
-* 2009-06-02: [http://www.freesoftwaremagazine.com/columns/extending_free_software_paradigm_diy_biology Extending the free software paradigm to DIY Biology] (freesoftwaremagazine.com)
-* 2009-06-01: [http://groups.google.com/group/diybio/msg/9bda9e3d613a18c6 The death of DIY Bio? Or the birth of a new cuisine....] (Gourmet Magazine)
-* 2009-05-18: [http://weekinideas.wordpress.com/2009/05/18/in-attics-and-closets-%e2%80%98biohackers%e2%80%99-prove-the-spirit-of-thomas-edison-endures/ In attics and closets, "biohackers" prove the spirit of Thomas Edison endures]
-* 2009-05-15: [http://futurismic.com/2009/05/15/garage-ribofunk-the-rise-of-homebrew-genetic-engineering/ Garage Ribofunk: The Rise of Homebrew Genetic Engineering]
-* 2009-05-14: [http://rt.com/Top_News/2009-05-14/Biohacking__harmless_hobby_or_global_threat.html?utm_source=2leep&utm_medium=2leep&utm_campaign=2leep Biohacking: harmless hobby or global threat?]
-* 2009-05-12: [http://online.wsj.com/article/SB124207326903607931.html In Attics and Closets, 'Biohackers' Discover Their Inner Frankenstein]
-* 2009-04-29: [http://singularityhub.com/2009/04/29/who-is-diybioorg/ Who is diybio.org?] (Singularity Hub)
-* 2009-04-28: [http://singularityhub.com/2009/04/28/do-it-yourself-biohacking/ Do-it-yourself biohacking] (Singularity Hub)
-* 2009-04-14: [http://jscms.jrn.columbia.edu/cns/2009-04-14/vaidyanathan-citizenscience.html What's in my closet? A biology lab] (JSCMS)
-* 2009-04-02: [http://diybio.org/2009/04/02/diybio-san-francisco-glow-in-the-dark-1/ DIYbio San Francisco - Glow in the Dark 1] (diybio.org)
-* 2009-03-18: [http://www.guardian.co.uk/technology/2009/mar/19/biohacking-genetics-research The Geneticist in the Garage] (The Guardian)
-* 2009-03-16: [http://groups.google.com/group/diybio/browse_thread/thread/3a1108bbb90d50e2 Genomeweb.com article] (?)
-* 2009-03-16: [http://scienceblogs.com/digitalbio/2009/03/diy_bio_programming_culture_an.php DIY bio, programming culture, and the cultural divide]
-* 2009-02-16: [http://www.labtimes.org/editorial/e_095.html Homemade Molecular Biology Labs aim to create Synthetic Life] (labtimes.org)
-* 2009-01-20: [http://biotechnewsupdate.blogspot.com/2009/01/biohacking-open-wetware-future.html Biohacking: The Open Wetware Future]
-* 2009-01-19: [http://www.wired.com/medtech/genetics/magazine/17-02/ff_diygenetics?currentPage=all DIY DNA: One Father's Attempt to Hack His Daughter's Genetic Code] (Wired)
-* 2009-01-07: [http://www.newscientist.com/article/mg20126881.400-genetic-manpulation-now-becoming-a-hobby.html?full=true&print=true Rise of the garage genome hackers] (New Scientist)
-* 2009-01-06: [http://blog.ginkgobioworks.com/2009/01/26/diy-bioengineering-recap-of-the-recent-mit-soapbox-session-on-diybio/ DIY bioengineering - recap of the recent MIT Soapbox session on DIYbio] (ginkgobioworks.com)
-* 2009-01-04: [http://scienceblogs.com/digitalbio/2009/01/diy_biology_projects_whats_you.php DIY biology projects - What's your motivation?] (scienceblogs.com)
-* 2009-01-01: [http://blog.makezine.com/archive/2009/01/diybio_for_biohackers.html DIYbio for biohackers] (makezine.com)
-* 2008-12-30: [http://www.pbs.org/newshour/bb/science/july-dec08/diybio_12-30.html Students, Scientists Build Biological Machines (transcript)] (Lehrer on PBS) ([http://www.youtube.com/watch?v=-IIWH6Hhcnc video])
-* 2008-12-29: [http://scienceblogs.com/purepedantry/2008/12/diy_bio_will_not_end_the_world.php DIY bio will not end the world]
-* 2008-12-25: [http://iht.nytimes.com/articles/ap/2008/12/25/america/Do-It-Yourself-DNA.php Amateurs are trying genetic engineering at home] ([http://science.slashdot.org/article.pl?sid=08/12/25/1833211 Slashdot])
-* 2008-12-18: [http://www.publico.es/ciencias/184626/biohackers/reventar/reinventar/biologia/garajes Público: Biohackers: reventar y reinventar la biología desde los garajes]
-* 2008-12-11: [http://seedmagazine.com/news/2008/12/the_biohacking_hobbyist.php The Biohacking Hobbyist] (Seed Magazine)
-* 2008-09-15: [http://blog.openwetware.org/community/2008/09/15/household-biohacking-coming-to-a-neighborhood-near-you/ Household biohacking coming to a neighborhood near you!] (blog.openwetware.org)
-* 2008-09-15: [http://www.boston.com/news/science/articles/2008/09/15/accessible_science/ Hackers aim to make biology household practice]
-* 2008-08-22: [http://www.nytimes.com/2008/08/22/science/22fish.html Fish Tale Has DNA Hook: Students Find Bad Labels] (NY Times)
-* 2008-06-13: [http://2020science.org/2008/06/13/8613-synthetic-biology-ethics-and-the-hacker-culture/ Synthetic biology, ethics and the hacker culture] (2020science.org)
-* 2008-06-06: [http://io9.com/5014059/a-homebrew-club-for-biogeeks A Homebrew Club for Biogeeks] (io9.com)
-* 2008-05-24: [http://diybio.org/2008/05/24/dont-phage-me-bro Don't Phage Me, Bro] (diybio.org)
-* 2008-05-19: [http://bookhling.wordpress.com/2008/05/19/diy-synthetic-biology/ DIY Synthetic Biology]
-* 2008-03-05: [http://pimm.wordpress.com/2008/03/05/the-conditions-of-a-mass-biotech-diy-movement/ The conditions of a mass biotech DIY movement]
-* 2007-11-06: [http://slog.thestranger.com/2007/11/homebrew_molecular_biology_club Homebrew Molecular Biology Club]
-* 2007-11-05: [http://seattletimes.nwsource.com/html/nationworld/2003994427_cancer05.html Patient's vision: Treating cancer without chemo]
-* 2007-11-04: [http://www.newsweek.com/id/68221 An Intel Approach to Meds]
-* 2007-07-19: [http://www.nybooks.com/articles/20370 Our Biotech Future] by Freeman Dyson (NY Books)
-* 2007-06-14: [http://www.huffingtonpost.com/michael-simmons/terrorizing-the-artists-i_b_52261.html Terrorizing the artists in the USA]
-* 2007-01-24: [http://pimm.wordpress.com/2007/01/24/what-is-biodiy/ What is BioDIY?]
-* 2006-08-18: [http://makezine.com/07/ Make Magazine: Backyard Biology] (Make Magazine)
-* 2006-04-23: [http://attilachordash.newsvine.com/_news/2006/04/23/155889-biotech-diyers-do-not-hesitate Biotech DIYers, do not hesitate]
-* 2005-05-01: [http://www.wired.com/wired/archive/13.05/view.html?pg=2 Splice it yourself] (Wired)
-* 2005-04-28: [http://www.fightaging.org/archives/2005/04/the-future-of-o.php The Future of Open Source Biotechnology] (fightaging.org)
-* 2004-06-02: [http://www.washingtonpost.com/wp-dyn/articles/A8278-2004Jun1.html Offbeat Materials at Professor's Home Set Off Bioterror Alarm] (Washington Post)
-* 2002-11-21: [http://pipeline.corante.com/archives/2002/11/21/the_future_and_its_friends.php The Future and its Friends] (In The Pipeline)
-* also, there have been many news stories from the pre-DIYbio era which should be in this list
 = What are some educational resources for DIYBio and Biology? What are all these terms and technologies DIYBio keeps discussing? =
@@ Line 501: / Line 309: @@
 See [http://openwetware.org/wiki/DIYbio/FAQ/Educational DIYBio FAQ: Education & Resources]
+== How can I grow and engineer yeast? How can I grow and engineer bacteria? ==
+See [http://openwetware.org/wiki/DIYbio/FAQ/Projects DIYBio FAQ: Projects]
 = What equipment do I need to perform DIYBio-related projects? =
@@ Line 532: / Line 343: @@
 * [http://www.google.com/search?hl=en&client=opera&rls=en&hs=078&num=100&q=site%3Aslashdot.org+open+source+hardware&btnG=Search other slashdot.org threads about OSH]
+=== Open Source Ecology ===
+Open source hardware includes large systems.
+  This is Chris Fornof with Open Source Ecology, http://opensourceecology.org
+  We're attempting to create a Global Village Construction Set
+  (GVCS, with the aim of creating a "civilization starter kit".
+  See the TED talk, http://www.ted.com/talks/marcin_jakubowski.html
 = Projects =
@@ Line 562: / Line 381: @@
 See [http://openwetware.org/wiki/DIYbio/FAQ/Methods DIYBio FAQ: Methods] for basic lab technique, including sterilization, using animals, etc.
+{{DIYbio:Top}}

DIYbio/FAQ: Difference between revisions

Latest revision as of 21:20, 5 September 2012

FAQ Revision History

What is DIYbio, as an organization?

What is DIYbio's mission?

Are we moving to a future where everyone performs a little genetic engineering? Is genetic engineering safe? Are GMO's safe? Is genetic engineering safe for hackers or everyone to perform? Aren't there too many risks or unknowns? Is it legal or illegal?

What are the social, ethical, legal/patent implications of DIY Bio or home genetic engineering? How might it be best to create more social, ethical, legal/patent discussion?

Are there Historical Precedents or Prior Cases which have Demonstrated these Issues?

U.S.

Arizona, 2009

Who is a "biohacker"?

How can I find out more and contribute?

Guidelines for Posting

Where can I see an archive of previous DIYbio discussions and questions?

Is there a group in my area?

Are there any videos from regional groups?

DIYbio MCR

DIYbio-NYC: Shot glass DNA extraction

DIYbio-SF: Tito's food coloring electrophoresis

DIYbio-boston: diybio visits the fablab

Bio-Artist Multimedia

What does a Garage Lab look like?

Has DIYbio been in the news?

What are some educational resources for DIYBio and Biology? What are all these terms and technologies DIYBio keeps discussing?

How can I grow and engineer yeast? How can I grow and engineer bacteria?

What equipment do I need to perform DIYBio-related projects?

What is open source hardware?

Open Source Ecology

Projects

What Projects has DIYBio completed? What projects are DIYBio contributors working on now? Who is working on what? Who do I contact to offer to collaborate on a project?

Appendix 1 - list of Synthetic Biology Companies

Appendix 2 - List of Equipment Suppliers

Appendix 3 - Laboratory Basics

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