DIYbio:Notebook/Open Gel Box 2.0/Transilluminator: Difference between revisions
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** 30 x 46cm USD $1750.00 | ** 30 x 46cm USD $1750.00 | ||
** 10 x 25cm USD $1250.00 | ** 10 x 25cm USD $1250.00 | ||
== DNA Dyes To Test == | |||
*Biotium | |||
**GelGreen | |||
**GelRed | |||
*LabSupplyMall | |||
**GR Safe | |||
**GR Safe II | |||
*Invitrogen | |||
**SYBR Green | |||
**SYBR Safe | |||
*Lonza | |||
**GelStar | |||
*Any Company | |||
**EtBR | |||
== Filter Wavelength Testing == | == Filter Wavelength Testing == | ||
Latest revision as of 03:12, 19 February 2009
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Author List
Norman Wang <wangn(at)hawaii.edu>
Purpose
For fluorescing cyanine type DNA dyes, using off the shelf light source and excitation/emission filters. DIY units should be significantly cheaper depending on selection of parts, labor, and design.
Commercial Units
- E-Gel® Safe Imager™ Real-Time Transilluminator USD $545.00
- Safe Imager™ blue light transilluminator USD $1135.00
- Dark Reader transilluminators
- 14 x 21cm USD $695.00
- 30 x 46cm USD $1750.00
- 10 x 25cm USD $1250.00
DNA Dyes To Test
- Biotium
- GelGreen
- GelRed
- LabSupplyMall
- GR Safe
- GR Safe II
- Invitrogen
- SYBR Green
- SYBR Safe
- Lonza
- GelStar
- Any Company
- EtBR
Filter Wavelength Testing
Spectraphotometer readings of various Tiffen glass, colored acrylic pre and post light filters. Image under Creative Commons BY-SA 3.0 License
Empirical testing of filter for cyanine type DNA dyes. Performance of each filter can clearly be seen between #1-#2 and #3 orange emission filter. Image under Creative Commons BY-SA 3.0 License
However colored acrylic #2 has visible unwanted fluorescence along the edge. Image under Creative Commons BY-SA 3.0 License
