DIYbio:Notebook/Open Gel Box 2.0/Transilluminator

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(New page: == Commercial Units == * E-Gel® Safe Imager™ Real-Time Transilluminator USD $545.00 * Safe Imager™ blue light transilluminator USD $1135.00 * Dark Reader transilluminators ** 14 x 21c...)
(added author attribution and a new filter testing picture)
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<html><a rel="license" href="http://creativecommons.org/licenses/by-sa/3.0/us/"><img alt="Creative Commons License" style="border-width:0" src="http://i.creativecommons.org/l/by-sa/3.0/us/88x31.png" /></a><br />This work is licensed under a <a rel="license" href="http://creativecommons.org/licenses/by-sa/3.0/us/">Creative Commons Attribution-Share Alike 3.0 License</a></html>
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== Author List ==
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Norman Wang <wangn(at)hawaii.edu>
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== Purpose ==
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For fluorescing cyanine type DNA dyes, using off the shelf light source and excitation/emission filters.  DIY units should be significantly cheaper depending on selection of parts, labor, and design.
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== Commercial Units ==
== Commercial Units ==
* E-Gel® Safe Imager™ Real-Time Transilluminator USD $545.00
* E-Gel® Safe Imager™ Real-Time Transilluminator USD $545.00
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== Filter Wavelength Testing ==
== Filter Wavelength Testing ==
[[Image:Gel_Box_20_Filter_Testing.jpg|none|480px]]
[[Image:Gel_Box_20_Filter_Testing.jpg|none|480px]]
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Spectraphotometer readings of various Tiffen glass, colored acrylic pre and post light filters. Image under [http://creativecommons.org/licenses/by-sa/3.0/ Creative Commons BY-SA 3.0 License]
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[[Image:Gel_Box_20_Filter_Testing_2.jpg|none|480px]]
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Empirical testing of filter for cyanine type DNA dyes. Performance of each filter can clearly be seen between #1-#2 and #3 orange emission filter. Image under [http://creativecommons.org/licenses/by-sa/3.0/ Creative Commons BY-SA 3.0 License]
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[[Image:Acrylic_Edge_Fluorescence.jpg|none|480px]]
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However colored acrylic #2 has visible unwanted fluorescence along the edge. Image under [http://creativecommons.org/licenses/by-sa/3.0/ Creative Commons BY-SA 3.0 License]

Revision as of 23:52, 9 February 2009

Creative Commons License
This work is licensed under a Creative Commons Attribution-Share Alike 3.0 License

Contents

Author List

Norman Wang <wangn(at)hawaii.edu>

Purpose

For fluorescing cyanine type DNA dyes, using off the shelf light source and excitation/emission filters. DIY units should be significantly cheaper depending on selection of parts, labor, and design.

Commercial Units

  • E-Gel® Safe Imager™ Real-Time Transilluminator USD $545.00
  • Safe Imager™ blue light transilluminator USD $1135.00
  • Dark Reader transilluminators
    • 14 x 21cm USD $695.00
    • 30 x 46cm USD $1750.00
    • 10 x 25cm USD $1250.00

Filter Wavelength Testing

Spectraphotometer readings of various Tiffen glass, colored acrylic pre and post light filters. Image under Creative Commons BY-SA 3.0 License

Empirical testing of filter for cyanine type DNA dyes. Performance of each filter can clearly be seen between #1-#2 and #3 orange emission filter. Image under Creative Commons BY-SA 3.0 License

However colored acrylic #2 has visible unwanted fluorescence along the edge. Image under Creative Commons BY-SA 3.0 License

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