DIYbio:Notebook/Open Thermal Cycler - Revision history

Carl Renneberg: /* Cost of current products */

2009-12-02T04:12:07Z

Cost of current products

←Older revision		Revision as of 04:12, 2 December 2009
Line 35:		Line 35:

	==Cost of current products==		==Cost of current products==
-	# Licensing -- [http://www.patentstorm.us/patents/6814934/fulltext.html florescent analysis is patented] although one key patent, [http://www.patentstorm.us/patents/6814934/fulltext.html US6814934], is under [http://www.genomeweb.com/pcrsample-prep/uspto-rejects-life-techs-thermal-cycler-patent-initial-ruling challenge] in a case before the ~~USPTO~~.	+	# Licensing -- [http://www.patentstorm.us/patents/6814934/fulltext.html florescent analysis is patented] although one key patent, [http://www.patentstorm.us/patents/6814934/fulltext.html US6814934], is under [http://www.genomeweb.com/pcrsample-prep/uspto-rejects-life-techs-thermal-cycler-patent-initial-ruling challenge] in a case before the [http://www.uspto.gov US Patent and Trademark Office]. The European equivalent to US6814934, [http://ep.espacenet.com/publicationDetails/biblio?adjacent=true&KC=A1&date=19981021&NR=0872562A1&DB=ep.espacenet.com&locale=en_GB&CC=EP&FT=D EP872562], has been confirmed by the [http://ep.espacenet.com/ European Patent Office] after [http://www.genomeweb.com/pcrsample-prep/uspto-rejects-life-techs-thermal-cycler-patent-initial-ruling-epo-upholds-europe challenges were dropped.]
		+
	#Hardware -- developed in house		#Hardware -- developed in house
	#Software -- developed in house		#Software -- developed in house

Carl Renneberg: /* Cost of current products */

2009-12-02T03:45:55Z

Cost of current products

←Older revision		Revision as of 03:45, 2 December 2009
Line 35:		Line 35:

	==Cost of current products==		==Cost of current products==
-	# Licensing -- [http://www.patentstorm.us/patents/6814934/fulltext.html florescent analysis is patented] although ~~this~~ is under [http://www.genomeweb.com/pcrsample-prep/uspto-rejects-life-techs-thermal-cycler-patent-initial-ruling challenge] in a case before the USPTO.	+	# Licensing -- [http://www.patentstorm.us/patents/6814934/fulltext.html florescent analysis is patented] although one key patent, [http://www.patentstorm.us/patents/6814934/fulltext.html US6814934], is under [http://www.genomeweb.com/pcrsample-prep/uspto-rejects-life-techs-thermal-cycler-patent-initial-ruling challenge] in a case before the USPTO.
	#Hardware -- developed in house		#Hardware -- developed in house
	#Software -- developed in house		#Software -- developed in house

Carl Renneberg: /* Cost of current products */

2009-12-02T03:40:32Z

Cost of current products

←Older revision		Revision as of 03:40, 2 December 2009
Line 35:		Line 35:

	==Cost of current products==		==Cost of current products==
-	# Licensing -- [http://www.patentstorm.us/patents/6814934/fulltext.html florescent analysis is patented] although this is under [http://www.genomeweb.com/pcrsample-prep/uspto-rejects-life-techs-thermal-cycler-patent-initial-ruling~~-epo-upholds-europe~~ challenge] in a case before the USPTO.	+	# Licensing -- [http://www.patentstorm.us/patents/6814934/fulltext.html florescent analysis is patented] although this is under [http://www.genomeweb.com/pcrsample-prep/uspto-rejects-life-techs-thermal-cycler-patent-initial-ruling challenge] in a case before the USPTO.
	#Hardware -- developed in house		#Hardware -- developed in house
	#Software -- developed in house		#Software -- developed in house

Carl Renneberg: /* Cost of current products */

2009-12-02T03:24:34Z

Cost of current products

←Older revision		Revision as of 03:24, 2 December 2009
Line 35:		Line 35:

	==Cost of current products==		==Cost of current products==
-	# Licensing -- [http://www.patentstorm.us/patents/6814934/fulltext.html florescent analysis is patented]	+	# Licensing -- [http://www.patentstorm.us/patents/6814934/fulltext.html florescent analysis is patented] although this is under [http://www.genomeweb.com/pcrsample-prep/uspto-rejects-life-techs-thermal-cycler-patent-initial-ruling-epo-upholds-europe challenge] in a case before the USPTO.
	#Hardware -- developed in house		#Hardware -- developed in house
	#Software -- developed in house		#Software -- developed in house

Tito: /* Comparisons to standard thermal cycling */

2009-05-11T02:19:55Z

Comparisons to standard thermal cycling

←Older revision		Revision as of 02:19, 11 May 2009
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	#(weak) Analysis of DNA		#(weak) Analysis of DNA

-	~~For most experimental processes~~, thermal cycling ~~of a sample~~ is not the final step. Gel electrophoresis, transformation into cells, and/or sequencing of the sample follow.	+	Since thermal cycling itself is not an analytical tool, thermal cycling is not the final step in most processes. Gel electrophoresis, transformation into cells, and/or sequencing of the sample follow.

	There are 2 general areas of quantitative thermal cycler use:		There are 2 general areas of quantitative thermal cycler use:
	#(strong) Synthesis/copying of DNA		#(strong) Synthesis/copying of DNA
-	#(strong) Quantitative and qualitative analysis of DNA	+	#(strong) Quantitative and qualitative analysis of DNA/RNA sample

-	qPCR on the other hand can be an all-in-one tool for analysis. In addition to ~~all~~ the functionality of a standard thermal cycler, a quantitative thermal cycler includes an imaging device to record the florescence of ~~the DNA~~ samples. Florescent markers added to each sample indicate the presence of a particular sequence (replacing gel electrophoresis and short sequencing) or measure gene expression (supplementing other analysis of modified cells)	+	qPCR on the other hand can be an all-in-one tool for analysis, both qualitatively and quantitatively. In addition to the functionality of a standard thermal cycler, a quantitative thermal cycler includes an imaging device to record the florescence of samples. Florescent markers added to each sample indicate the presence of a particular sequence (replacing gel electrophoresis and short sequencing) or measure gene expression (supplementing other analysis of modified cells)

	==Cost of current products==		==Cost of current products==

Tito: /* Project Description/Abstract */

2009-05-09T23:10:45Z

Project Description/Abstract

←Older revision		Revision as of 23:10, 9 May 2009
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	\|colspan="2"\|		\|colspan="2"\|
	==Project Description/Abstract==		==Project Description/Abstract==
-	~~Open Thermal Cycler~~	+
		+	:Draft:
		+	==Why quantitative thermal cycling?==
		+	How do you go from a spit sample to - or from a crosswalk swab to a map of the bacterial populations of the world? Can $1,000 get you there? quantitative thermal cycling can be an all-in-one device capable
		+
		+	==Comparisons to standard thermal cycling==
		+	The following is the outline of quantitative thermal cycling, its advantages over traditional thermal cycling,
		+
		+	There are 2 general areas of thermal cycler use:
		+	#(strong) Synthesis/copying of DNA
		+	#(weak) Analysis of DNA
		+
		+	For most experimental processes, thermal cycling of a sample is not the final step. Gel electrophoresis, transformation into cells, and/or sequencing of the sample follow.
		+
		+	There are 2 general areas of quantitative thermal cycler use:
		+	#(strong) Synthesis/copying of DNA
		+	#(strong) Quantitative and qualitative analysis of DNA
		+
		+	qPCR on the other hand can be an all-in-one tool for analysis. In addition to all the functionality of a standard thermal cycler, a quantitative thermal cycler includes an imaging device to record the florescence of the DNA samples. Florescent markers added to each sample indicate the presence of a particular sequence (replacing gel electrophoresis and short sequencing) or measure gene expression (supplementing other analysis of modified cells)
		+
		+	==Cost of current products==
		+	# Licensing -- [http://www.patentstorm.us/patents/6814934/fulltext.html florescent analysis is patented]
		+	#Hardware -- developed in house
		+	#Software -- developed in house

	==Details==		==Details==

Bill Flanagan: /* Details */

2009-04-24T22:41:24Z

Details

←Older revision		Revision as of 22:41, 24 April 2009
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	[[DIYbio:Notebook/Open_Thermal_Cycler/Specifications \| Specifications]] - what hardware, software, GUI-ideas we might use to build it (i.e. Arduino, or a checkbox interface for entering temperatures)		[[DIYbio:Notebook/Open_Thermal_Cycler/Specifications \| Specifications]] - what hardware, software, GUI-ideas we might use to build it (i.e. Arduino, or a checkbox interface for entering temperatures)
		+
		+	[[DIYbio:Notebook/Open_Thermal_Cycler/Conference Calls/2009/4/26 \| 4/26/2009 Conference Call]] - Details for connecting to the conference call.

	\|-		\|-

Tito: /* Details */

2009-04-24T07:05:17Z

Details

←Older revision		Revision as of 07:05, 24 April 2009
Line 17:		Line 17:

	==Details==		==Details==
-	[[DIYbio:Notebook/Open_Thermal_Cycler/Use_Cases \| Use Cases - experiments involving a thermal cycler]]	+	[[DIYbio:Notebook/Open_Thermal_Cycler/Use_Cases \| Use Cases ]]- experiments involving a thermal cycler

	[[DIYbio:Notebook/Open_Thermal_Cycler/Requirements \| Requirements]]- what a user might want to do with an Open Thermal Cycler (i.e. get more DNA)		[[DIYbio:Notebook/Open_Thermal_Cycler/Requirements \| Requirements]]- what a user might want to do with an Open Thermal Cycler (i.e. get more DNA)

Tito: /* Details */

2009-04-24T07:04:59Z

Details

←Older revision		Revision as of 07:04, 24 April 2009
Line 17:		Line 17:

	==Details==		==Details==
-	[[DIYbio:Notebook/Open_Thermal_Cycler/Use_Cases \| Use Cases]]	+	[[DIYbio:Notebook/Open_Thermal_Cycler/Use_Cases \| Use Cases - experiments involving a thermal cycler]]

	[[DIYbio:Notebook/Open_Thermal_Cycler/Requirements \| Requirements]]- what a user might want to do with an Open Thermal Cycler (i.e. get more DNA)		[[DIYbio:Notebook/Open_Thermal_Cycler/Requirements \| Requirements]]- what a user might want to do with an Open Thermal Cycler (i.e. get more DNA)

Tito at 07:03, 24 April 2009

2009-04-24T07:03:31Z

←Older revision		Revision as of 07:03, 24 April 2009
Line 17:		Line 17:

	==Details==		==Details==
		+	[[DIYbio:Notebook/Open_Thermal_Cycler/Use_Cases \| Use Cases]]
		+
	[[DIYbio:Notebook/Open_Thermal_Cycler/Requirements \| Requirements]]- what a user might want to do with an Open Thermal Cycler (i.e. get more DNA)		[[DIYbio:Notebook/Open_Thermal_Cycler/Requirements \| Requirements]]- what a user might want to do with an Open Thermal Cycler (i.e. get more DNA)